Restricted accessResearch articleFirst published online 2025-8
In situ assembly of Ag@M-TiO 2 nanoparticles grafted on cotton fabrics with durable antibacterial and hemostatic properties for promoting bacteria-infected wound healing
Uncontrolled wound bleeding and bacterial infection are the most common problems in wound healing/skin regeneration, and failure to provide effective care will obviously increase the risk of other diseases and reduce the quality of wound healing. The ideal strategy is to prepare a simple dressing with rapid hemostasis and durable antimicrobial activity as an alternative to conventional hemostatic gauze to facilitate satisfactory wound healing. In this study, Ag-doped TiO2 nanoparticles modified with unsaturated carbon–carbon double bonds (Ag@M-TiO2) were synthesized and grafted onto the surface of cotton fabrics by chemical finishing. Compared with the common cotton fabrics, the Ag@M-TiO2 modified cotton fabrics (C-Ag@TiO2) had a higher blood affinity and were able to absorb spilled blood and achieve rapid hemostasis by aggregating erythrocytes, stimulating blood platelets and activating hemostasis-related pathways. The mouse liver internal hemorrhage experiment confirmed that the wound could achieve rapid hemostasis within 30 s. In addition, the C-Ag@TiO2 dressings exhibited broad spectrum antimicrobial properties (>99%) and could promote wound healing by preventing bacterial infection (the wound closure ratio was almost 100% after 14 days). The study demonstrated the important role of the multifunctional C-Ag@TiO2 dressings in healing infected wound and controlling internal bleeding. It provides a potential strategy for wound healing and skin regeneration to replace traditional cotton gauze.
ZengQQiXShiG, et al.
Wound dressing: from nanomaterials to diagnostic dressings and healing evaluations. ACS Nano2022;
16: 1708–1733. DOI: 10.1021/acsnano.1c08411.
2.
LuXLiXYuJ, et al.
Nanofibrous hemostatic materials: Structural design, fabrication methods, and hemostatic mechanisms. Acta Biomater2022;
154: 49–62. DOI: 10.1016/j.actbio.2022.10.028.
3.
HongYZhouFHuaY, et al.
A strongly adhesive hemostatic hydrogel for the repair of arterial and heart bleeds. Nat Commun2019;
10: 2060. DOI: 10.1038/s41467-019-10004-7.
4.
KimKRyuJHKohMY, et al.
Coagulopathy-independent, bioinspired hemostatic materials: A full research story from preclinical models to a human clinical trial. Sci Adv2021;
7: eabc9992. DOI: 10.1126/sciadv.abc9992.
5.
WangYZhaoYQiaoL, et al.
Cellulose fibers-reinforced self-expanding porous composite with multiple hemostatic efficacy and shape adaptability for uncontrollable massive hemorrhage treatment. Bioact Mater2021;
6: 2089–2104. DOI: 10.1016/j.bioactmat.2020.12.014.
6.
LiangYHeJGuoB.Functional hydrogels as wound dressing to enhance wound healing. ACS Nano2021;
15: 12687–12722. DOI: 10.1021/acsnano.1c04206.
7.
ZhaoPFengYZhouY, et al.
Gold@Halloysite nanotubes-chitin composite hydrogel with antibacterial and hemostatic activity for wound healing. Bioact Mater2023;
20: 355–367. DOI: 10.1016/j.bioactmat.2022.05.035.
8.
YuanHChenLHongFF.A biodegradable antibacterial nanocomposite based on oxidized bacterial nanocellulose for rapid hemostasis and wound healing. ACS Appl Mater Inter2019;
12: 3382–3392. DOI: 10.1021/acsami.9b17732.
9.
ShabanovaEMDrozdovASFakhardoAF, et al.
Thrombin@Fe3O4 nanoparticles for use as a hemostatic agent in internal bleeding. Sci Rep UK2018;
8: 233. DOI: 10.1038/s41598-017-18665-4.
10.
KuddushiMShahAAAyranciC, et al.
Recent advances in novel materials and techniques for developing transparent wound dressings. J Mater Chem B2023;
11: 6201–6224. DOI: 10.1039/d3tb00639e.
11.
LiSGuBLiX, et al.
MXene‐enhanced chitin composite sponges with antibacterial and hemostatic activity for wound healing. Adv Healthc Mater2022;
11: 2102367. DOI: 10.1002/adhm.202102367.
12.
GuoBDongRLiangY, et al.
Haemostatic materials for wound healing applications. Nat Rev Chem2021;
5: 773–791. DOI: 10.1038/s41570-021-00323-z.
13.
WangLChenMCaiR, et al.
Surface engineered long-lasting antibacterial Janus cotton fabrics with excellent moisture/thermal management properties. Chem Eng J2023;
475. DOI: 10.1016/j.cej.2023.146386.
14.
QianJDongQChunK, et al.
Highly stable, antiviral, antibacterial cotton textiles via molecular engineering. Nat Nanotechnol2022;
18: 168–176. DOI: 10.1038/s41565-022-01278-y.
15.
FengYHeYLinX, et al.
Assembly of clay nanotubes on cotton fibers mediated by biopolymer for robust and high‐performance hemostatic dressing. Adv Healthc Mater2022;
12. DOI: 10.1002/adhm.202202265.
16.
TavakoliSKlarAS.Advanced hydrogels as wound dressings. Biomolecules2020;
10: 1169. DOI: 10.3390/biom10081169.
17.
ZhangYSKhademhosseiniA.Advances in engineering hydrogels. Science2017;
356. DOI: 10.1126/science.aaf3627.
18.
CaoYCongHYuB, et al.
A review on the synthesis and development of alginate hydrogels for wound therapy. J Mater Chem B2023;
11: 2801–2829. DOI: 10.1039/d2tb02808e.
19.
FanXYangFNieC, et al.
Biocatalytic nanomaterials: a new pathway for bacterial disinfection. Adv Mater2021;
33: 2100637. DOI: 10.1002/adma.202100637.
20.
XiGLiuWChenM, et al.
Polysaccharide-based lotus seedpod surface-like porous microsphere with precise and controllable micromorphology for ultrarapid hemostasis. ACS Appl Mater Inter2019;
11: 46558–46571. DOI: 10.1021/acsami.9b17543.
21.
JiangJLiXLiH, et al.
Recent progress in nanozymes for the treatment of diabetic wounds. J Mater Chem B2023;
11: 6746–6761. DOI: 10.1039/d3tb00803g.
22.
LiSChenAChenY, et al.
Lotus leaf inspired antiadhesive and antibacterial gauze for enhanced infected dermal wound regeneration. Chem Eng J2020;
402: 126202. DOI: 10.1016/j.cej.2020.126202.
23.
XiangJZhuRLangS, et al.
Mussel-inspired immobilization of zwitterionic silver nanoparticles toward antibacterial cotton gauze for promoting wound healing. Chem Eng J2021;
409: 128291. DOI: 10.1016/j.cej.2020.128291.
24.
QiXHuangYYouS, et al.
Engineering robust Ag‐decorated polydopamine nano‐photothermal platforms to combat bacterial infection and prompt wound healing. Adv Sci2022;
9: 2106015. DOI: 10.1002/advs.202106015.
25.
JiaQSongQLiP, et al.
Rejuvenated photodynamic therapy for bacterial infections. Adv Healthc Mater2019;
8: 1900608. DOI: 10.1002/adhm.201900608.
26.
MalekiAHeJBochaniS, et al.
Multifunctional photoactive hydrogels for wound healing acceleration. ACS Nano2021;
15: 18895–18930. DOI: 10.1021/acsnano.1c08334.
27.
GnanasekarSKasiGHeX, et al.
Recent advances in engineered polymeric materials for efficient photodynamic inactivation of bacterial pathogens. Bioact Mater2023;
21: 157–174. DOI: 10.1016/j.bioactmat.2022.08.011.
28.
ImaniSMLadouceurLMarshallT, et al.
Antimicrobial nanomaterials and coatings: current mechanisms and future perspectives to control the spread of viruses including SARS-CoV-2. ACS Nano2020;
14: 12341–12369. DOI: 10.1021/acsnano.0c05937.
29.
WangJZhangCYangY, et al.
Poly (vinyl alcohol) (PVA) hydrogel incorporated with Ag/TiO2 for rapid sterilization by photoinspired radical oxygen species and promotion of wound healing. Appl Surf Sci2019;
494: 708–720. DOI: 10.1016/j.apsusc.2019.07.224.
30.
HouXMaHLiuF, et al.
Synthesis of Ag ion-implanted TiO2 thin films for antibacterial application and photocatalytic performance. J Hazard Mater2015;
299: 59–66. DOI: 10.1016/j.jhazmat.2015.05.014.
31.
Esteban FlorezFLHiersRDLarsonP, et al.
Antibacterial dental adhesive resins containing nitrogen-doped titanium dioxide nanoparticles. Mat Sci Eng C-Mater2018;
93: 931–943. DOI: 10.1016/j.msec.2018.08.060.
32.
YangGYinHLiuW, et al.
Synergistic Ag/TiO2-N photocatalytic system and its enhanced antibacterial activity towards Acinetobacter baumannii. Appl Catal B-Environ2018;
224: 175–182. DOI: 10.1016/j.apcatb.2017.10.052.
33.
LiuMWangXCuiJ, et al.
Electrospun flexible magnesium-doped silica bioactive glass nanofiber membranes with anti-inflammatory and pro-angiogenic effects for infected wounds. J Mater Chem B2023;
11: 359–376. DOI: 10.1039/d2tb02002e.
34.
QiuWWangQLiM, et al.
Peptidoglycan-inspired peptide-modified injectable hydrogels with enhanced elimination capability of bacterial biofilm for chronic wound healing. Compos Part B-Eng2021;
227: 109402. DOI: 10.1016/j.compositesb.2021.109402.
35.
XuanXZhouYChenA, et al.
Silver crosslinked injectable bFGF-eluting supramolecular hydrogels speed up infected wound healing. J Mater Chem B2020;
8: 1359–1370. DOI: 10.1039/c9tb02331c.
36.
ZhuJHanHLiF, et al.
Self-assembly of amino acid-based random copolymers for antibacterial application and infection treatment as nanocarriers. J Colloid Interface Sci2019;
540: 634–646. DOI: 10.1016/j.jcis.2018.12.091.
37.
ZhuJHanHLiF, et al.
Peptide-functionalized amino acid-derived pseudoprotein-based hydrogel with hemorrhage control and antibacterial activity for wound healing. Chem Mater2019;
31: 4436–4450. DOI: 10.1021/acs.chemmater.9b00850.
38.
HanHZhuJWuD, et al.
Inherent guanidine nanogels with durable antibacterial and bacterially antiadhesive properties. Adv Funct Mater2019;
29: 1806594. DOI: 10.1002/adfm.201806594.
39.
LiWDongKRenJ, et al.
A β‐lactamase‐imprinted responsive hydrogel for the treatment of antibiotic‐resistant bacteria. Angew Chem Int Ed2016;
55: 8049–8053. DOI: 10.1002/anie.201600205.
40.
ZhangHChenCZhangH, et al.
Janus medical sponge dressings with anisotropic wettability for wound healing. Appl Mater Today2021;
23: 101068. DOI: 10.1016/j.apmt.2021.101068.
41.
ZhuJQiuWYaoC, et al.
Water-stable zirconium-based metal-organic frameworks armed polyvinyl alcohol nanofibrous membrane with enhanced antibacterial therapy for wound healing. J Colloid Interface Sci2021;
603: 243–251. 2021/06/30. DOI: 10.1016/j.jcis.2021.06.084.
42.
LiuYHouCJiaoT, et al.
Self-assembled AgNP-containing nanocomposites constructed by electrospinning as efficient dye photocatalyst materials for wastewater treatment. Nanomaterials2018;
8: 35. DOI: 10.3390/nano8010035.
43.
ChoiYKooMSBokareAD, et al.
Sequential process combination of photocatalytic oxidation and dark reduction for the removal of organic pollutants and Cr(VI) using Ag/TiO2. Environ Sci Technol2017;
51: 3973–3981. DOI: 10.1021/acs.est.6b06303.
44.
LiNPranantyoDKangE, et al.
In situ self-assembled polyoxotitanate cages on flexible cellulosic substrates: multifunctional coating for hydrophobic, antibacterial, and UV-blocking applications. Adv Funct Mater2018;
28: 1800345. DOI: 10.1002/adfm.201800345.
45.
ChenSYuanLLiQ, et al.
Durable antibacterial and nonfouling cotton textiles with enhanced comfort via zwitterionic sulfopropylbetaine coating. Small2016;
12: 3516–3521. DOI: 10.1002/smll.201600587.
46.
SmithMKMiricaKA.Self-organized frameworks on textiles (SOFT): conductive fabrics for simultaneous sensing, capture, and filtration of gases. J Am Chem Soc2017;
139: 16759–16767. DOI: 10.1021/jacs.7b08840.
47.
NgoVGBressyCLerouxC, et al.
Synthesis of hybrid TiO2 nanoparticles with well-defined poly(methyl methacrylate) and poly(tert-butyldimethylsilyl methacrylate) via the RAFT process. Polymer2009;
50: 3095–3102. DOI: 10.1016/j.polymer.2009.04.077.
48.
BachlerGvon GoetzNHungerbuhlerK.Using physiologically based pharmacokinetic (PBPK) modeling for dietary risk assessment of titanium dioxide (TiO2) nanoparticles. Nanotoxicology2015;
9: 373–380. DOI: 10.3109/17435390.2014.940404.
49.
ZhaoWDingQZhouB, et al.
Nitric oxide‐actuated titanium dioxide janus nanoparticles for enhanced multimodal disruption of infectious biofilms. Adv Funct Mater2024. DOI: 10.1002/adfm.202407626.
50.
FuCFanYLiuG, et al.
One-step fabrication of an injectable antibacterial collagen hydrogel with in situ synthesized silver nanoparticles for accelerated diabetic wound healing. Chem Eng J2024;
480. DOI: 10.1016/j.cej.2023.148288.
51.
ZhaoJLiWLiL, et al.
Biomimetic Ag-modified core-shell nanofibrous membrane with enhanced solar absorption and water replenishment for efficient clean water production and antibacterial activity. Sep Purif Technol2025; 354. DOI: 10.1016/j.seppur.2024.129084.
52.
SweetDGCarnielliVGreisenG, et al.
European consensus guidelines on the management of respiratory distress syndrome-2016 update. Neonatology2017;
111: 107–125. DOI: 10.1159/000448985.
53.
MaoCXiangYLiuX, et al.
Photo-inspired antibacterial activity and wound healing acceleration by hydrogel embedded with Ag/Ag@AgCl/ZnO nanostructures. ACS Nano2017;
11: 9010–9021. DOI: 10.1021/acsnano.7b03513.
54.
WangZLyuTXieQ, et al.
Shape-adapted self-gelation hydrogel powder for high-performance hemostasis and wound healing. Appl Mater Today2023;
35. DOI: 10.1016/j.apmt.2023.101948.
55.
HsuBBConwayWTschabrunnCM, et al.
Clotting mimicry from robust hemostatic bandages based on self-assembling peptides. ACS Nano2015;
9: 9394–9406. DOI: 10.1021/acsnano.5b02374.
56.
LanGLiQLuF, et al.
Improvement of platelet aggregation and rapid induction of hemostasis in chitosan dressing using silver nanoparticles. Cellulose2019;
27: 385–400. DOI: 10.1007/s10570-019-02795-1.
57.
QuJZhaoXLiangY, et al.
Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints skin wound healing. Biomaterials2018;
183: 185–199. DOI: 10.1016/j.biomaterials.2018.08.044.
