This study explores the potential of Acalypha indica and Tectona grandis leaf extracts in wound-dressing applications, emphasizing the development of natural, bio-based treatment options. Using ultrasonic-assisted extraction (UAE) with ethanol, the extracts were coated onto bamboo woven fiber fabric at varying concentrations (50%, 75%, and 100%). Comprehensive characterization of bioactive compounds was conducted through phytochemical analysis, thin layer chromatography (TLC), antioxidant assays (FRAP), gas chromatography mass spectrometry (GC-MS), and Fourier-transform infrared (FTIR) spectroscopy. The coated fabric exhibited effective antibacterial properties against Bacillus subtilis and Escherichia coli. In addition, tests on fragrance, color intensity, and olfaction showed that the treated fabric maintained a pleasant herbal aroma lasting up to 20 washes. To further investigate the role of bamboo fabric as a substrate, an in vivo wound healing assay was performed on Wistar rats over 21 days. The 75% and 100% extract concentrations demonstrated over 90% wound closure, with the fastest healing time being 17 days. These results highlight that bio-extract-treated bamboo fabric not only promotes wound healing but also offers textile-related advantages, positioning it as a promising candidate for functional healthcare textiles.
YengNKShaariRNordinML, et al.
Investigation of wound healing effect of Acalypha indica extract in Sprague Dawley rats. Biomed Pharmacol J2019;
12: 1857–1865.
2.
RodriguesMKosaricNBonhamCA, et al.
Wound healing: a cellular perspective. Physiol Rev2019;
99: 665–706.
3.
Monaco JL, Lawrence WT. Acute wound healing an overview. Clin Plast Surg 2003; 30(1): 1–12. PMID: 12636211.
4.
Sheokand B, Vats M, Kumar A, et al. Natural polymers used in the dressing materials for wound healing: Past, present and future. Journal of Polymer Science 2023; 61(14): 1389–1414. https://doi.org/10.1002/pol.20220734
5.
BernhoftA.A Brief Review on Bioactive Compounds in Plants.
Oslo:
The Norwegian Academy of Science and Letters, 2010.
6.
ZaynabMFatimaMAbbasS, et al.
Role of secondary metabolites in plant defense against pathogens. Microbial Pathogen2018;
124: 198–202.
7.
ZahidinNSSaidinSZulkifliRM, et al.
A review of Acalypha indica L. (Euphorbiaceae) as traditional medicinal plant and its therapeutic potential. J Ethnopharmacol2017;
207: 146–173.
8.
Nidavani, Ramesh & AM, Mahalakshmi. Teak (Tectona grandis Linn.): A renowned timber plant with potential medicinal values. International Journal of Pharmacy and Pharmaceutical Sciences 2014; 6: 48–54.
9.
RathodA.Analysis of physical characteristics of bamboo fabrics. Int J Res Eng Technol2014;
3: 21–25.
10.
PrakashCSukumarNRameshP, et al.
Development and characterization of wound dressing material coated with natural extracts of curcumin, aloe vera and chitosan solution enhanced with rhEGF (REGEN-DTM). J Nat Fibers2020;
18: 2019–2032.
11.
SorescuA-ANutaAIonR-M, et al. Qualitative analysis of phytochemicals from sea buckthorn and gooseberry. Epub ahead of print November 7, 2018. DOI: 10.5772/intechopen.77365.
12.
Dobjanschi L, Zdrinca M, Muresan, M, et al. The thin layer chromatography analysis of saponins belonging to Solidago species. Fasc Prot Mediu 2013; 21: 56–60.
13.
SyarifahALRetnowatiRSoebiantoroS.Characterization of secondary metabolites profile of flavonoid from salam leaves (Eugenia polyantha) using TLC and UV spectrophotometry. Pharm Sci Res2019;
6. DOI: 10.7454/psr.v6i3.4219.
14.
KristantiHWasT.Detection of alkaloid, flavonoid, and terpenoid compounds in bread (Artocarpus communis Forst.) leaves and pulps. KnE Life Sci2015;
2: 129.
15.
MariswamyYGnarajWEJohnsonM.Chromatographic finger print analysis of steroids in Aerva lanata L by HPTLC technique. Asian Pacific J Trop Biomed2011;
1: 428–433.
16.
SakthiguruNManimohanMJaganathanG, et al.
Biologically active chitosan/ZnO/Acalypha indica leaf extract biocomposite: an investigation of antibacterial, cell proliferation and cell migration aptitude for wound healing application. Sustain Chem Pharm2021;
19: 100357.
17.
GuchuBMMachochoAKMwihiaSK, et al.
In vitro antioxidant activities of methanolic extracts of Caesalpinia volkensii Harms.,Vernonia lasiopus O. Hoffm., and Acacia hockii De Wild. Evidence-based Complement Altern Med2020;
2020: 1–10.
18.
BinsuwaidanRElekhnawyEElseadyWS, et al.
Antibacterial activity and wound healing potential of Cycas thouarsii R.Br n-butanol fraction in diabetic rats supported with phytochemical profiling. Biomed Pharmacother2022;
155: 113763.
19.
TangAWangYLeeC, et al.
Computer color matching and levelness of PEG-based reverse micellar decamethyl cyclopentasiloxane (D5) solvent-assisted reactive dyeing on cotton fiber. Appl Sci2017;
7: 682.
20.
DasSDasASelvanRT, et al.
The antibacterial and aroma finishing of cotton fabrics by Mentha pipertia extract. J Text Inst2020;
112: 1181–1190.
21.
LodhiSSinghaiA.Preliminary pharmacological evaluation of Martynia annua Linn leaves for wound healing. Asian Pacific J Trop Biomed2011;
1: 421–427.
22.
YadavRNSAgarwalaM.Phytochemical analysis of some medicinal plants. J Phytol2011;
3: 10–14.
23.
KhatoonAIslamTHossainM.
Biological activities of secondary metabolites from medicinal plants: A review on antioxidant, anti-inflammatory, and antimicrobial properties. Nat Prod Res2020;
34(18): 2604–2612.
24.
ChenH, et al.
Chromatographic analysis and identification of bioactive compounds from natural sources. J Chromatograp B2021;
1176: 122714.
25.
BhaleA, et al.
Nanocomposites for food packaging: a review on materials and techniques. Phytomedicine2019;
55: 159–166.
26.
YadavS, et al.
Phytochemical screening and antimicrobial activity of medicinal plants: a review. Asian J Res Chem2020;
13(2): 87–93.
27.
SinghVKaurKKaurS, et al.
Trimethoxyflavones from Ocimum basilicum L. leaves improve long term memory in mice by modulating multiple pathways. J Ethnopharmacol2022;
295: 115438. DOI: 10.1016/j.jep.2022.115438
28.
ChowdhuryS, et al.
Fabrication of composite materials from bio-based fibers for enhanced properties. Compos Sci Technol2020;
192: 108118.
29.
ZhaoX, et al.
Food and medicinal properties of plant extracts: advances and challenges. Food Chem2021;
345: 128665.
30.
ChandNMohammedF.Tribology of natural fiber polymer composites. Epub ahead of print January 1, 2008. DOI: 10.1533/9781845695057.
31.
WangQZhangYLiangW, et al.
Effect of silane treatment on mechanical properties and thermal behavior of bamboo fibers reinforced polypropylene composites. J Eng Fibers Fabrics2020;
15: 155892502095819.
32.
VaidyaVIngoldKUPrattDA.Garlic: source of the ultimate antioxidants—sulfenic acids. Angew Chem2008;
48: 157–160.
33.
ChandrasekaranMKannathasanKVenkatesaluV.Antimicrobial activity of fatty acid methyl esters of some members of Chenopodiaceae. Z Naturforsch C2008;
63: 331–336.
34.
Pavia DL, Lampman GM, & Kriz GS. (2009). Introduction to spectroscopy. Thomson Brooks/Cole.
35.
SaniN, et al.
Antimicrobial properties of Azadirachta indica extracts: an overview. Pharmacognosy Rev2020;
14(28): 123–129.
36.
BasuA, et al.
Understanding the difference between Gram-positive and Gram-negative bacteria in the context of antimicrobial resistance. Infect Control Hosp Epidemiol2021;
42(3): 287–291.
37.
TeklaniPPereraB.The important biological activities and phytochemistry of Acalypha indica. Int J Res Pharm Sci2016;
6(1): 30–35.
38.
GoswamiDNirmalSPatilM, et al.
An overview of Tectona grandis: chemistry and pharmacological profile. Pharmacognosy Rev2009;
3: 170–174.
39.
ChekuriSLingfaLPanjalaS, et al.
Acalypha indica L. – an important medicinal plant: a brief review of its pharmacological properties and restorative potential. Eur J Med Plants2020. DOI: 10.9734/ejmp/2020/v31i1130294
40.
RamaiahGBAriAP. Evaluation of color strength (K/S) values of cotton fabrics dyed with reactive dye and treated with silver nanoparticles. AIP Conference Proceedings. Epub ahead of print January 1, 2019. DOI: 10.1063/1.5130321.
41.
TakleVVSavadRVKandalkarAM, et al.
Pharmacognostic and phytochemical investigations of aerial parts of Acalypha indica Linn. Pharmacognosy J2011;
3: 33–35.
42.
GaneshkumarMPonrasuTKrithikaR, et al.
Topical application of Acalypha indica accelerates rat cutaneous wound healing by up-regulating the expression of Type I and III collagen. J Ethnopharmacol2012;
142: 14–22.
