An optimized co-formulation minimized quaternary ammonium compounds adsorption onto raw cotton disposable disinfecting wipes and maintained efficacy against methicillin-resistant Staphylococcus aureus,vancomycin-resistant Enterococcus faecalis,and Pseudomonas aeruginosa

Abstract

The transmission of infectious agents can occur through secondary routes by contact with contaminated inanimate objects in clinical and food service settings. Effective disinfection of exposed surfaces can aid in reducing secondary transmission of infectious agents. Quaternary ammonium compounds (quats) are biocides widely used as active ingredients in disinfecting solutions and are effective against a wide range of microorganisms, including those with antibiotic resistance. Cellulosic fibers such as cotton are desirable as disposable substrates for the application of disinfecting solutions, since they have good absorbency and cleaning properties and are sustainable and biodegradable. However, cotton fibers deplete quats from solution through strong ionic interactions at the solid–liquid interface, thereby reducing the amount of quat deposited onto a surface for effective disinfection. In this study, we used response surface methodology to model the depletion of the quat, alkyldimethylbenzylammonium chloride (ADBAC), onto cotton wipe substrates in the presence of chemical compounds that interfered or competed with the ionic interactions between the quat and the cotton fiber surface. Preliminary efficacy testing successfully demonstrated that an optimized disinfecting ADBAC co-formulation applied with a raw cotton disposable wipe maintained efficacy against representative gram negative (Pseudomonas aeruginosa) and gram positive (Staphylococcus aureus) bacteria compared to a control disinfecting solution containing only ADBAC. Our optimized disinfecting ADBAC co-formulation remained efficacious against Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, and vancomycin-resistant Enterococcus faecalis, demonstrating for the first time that quat adsorption onto cotton substrates can be minimized and efficacy maintained with inexpensive added chemistries.

Keywords

disinfectant cotton quaternary ammonium compounds wipes

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References

Klevens

Edwards

Richards

Jr et al.

Estimating health care-associated infections and deaths in US hospitals, 2002. Publ Health Rep 2007; 122: 160–166.

Magill

Edwards

Bamberg

et al.

Multistate point-prevalence survey of health care–associated infections. New Engl J Med 2014; 370: 1198–1208.

Siegel

Rhinehart

Jackson

et al.

Management of multidrug-resistant organisms in health care settings, 2006. Am J Infect Contr 2007; 35: S165–S93.

Otter

Yezli

French

. The role played by contaminated surfaces in the transmission of nosocomial pathogens. Infect Contr Hosp Epidemiol 2011; 32: 687–699.

Wagenvoort

De Brauwer

Penders

et al.

Environmental survival of vancomycin-resistant Enterococcus faecium. J Hosp Infect 2011; 77: 282–283.

Wagenvoort

Sluijsmans

Penders

. Better environmental survival of outbreak vs. sporadic MRSA isolates. J Hosp Infect 2000; 45: 231–234.

Fijan

Pahor

Šostar Turk

. Survival of Enterococcus faecium, Staphylococcus aureus and Pseudomonas aeruginosa on cotton. Text Res J. 2017; 87: 1711–1721.

Rutala

Weber

. Disinfection and sterilization: an overview. Am J Infect Contr 2013; 41: S2–S5.

Fijan

Turk

SŠ

. Hospital textiles, are they a possible vehicle for healthcare-associated infections? Int J Environ Res Publ Health 2012; 9: 3330–3343.

10.

Ackerley

. The key role of hygiene and cleanliness in the domestic environment. Perspect Publ Health 2016; 136: 210–212.

11.

Sattar

Bradley

Kibbee

et al.

Disinfectant wipes are appropriate to control microbial bioburden from surfaces: use of a new ASTM standard test protocol to demonstrate efficacy. J Hosp Infect 2015; 91: 319–325.

12.

Weber

Rutala

Sickbert-Bennett

. Outbreaks associated with contaminated antiseptics and disinfectants. Antimicrob Agents Chemother 2007; 51: 4217–4224.

13.

Valenzuela

Benomar

Abriouel

et al.

Biocide and copper tolerance in enterococci from different sources. J Food Protect 2013; 76: 1806–1809.

14.

Fazlara

Ekhtelat

. The disinfectant effects of benzalkonium chloride on some important foodborne pathogens. Am Euras J Agric Environ Sci 2012; 12: 23–29.

15.

Nam

Slopek

Wolf

et al.

Comparison of biodegradation of low-weight hydroentangled raw cotton nonwoven fabric and that of commonly used disposable nonwoven fabrics in aerobic Captina silt loam soil. Text Res J 2016; 86: 155–166.

16.

Gold

Hitchins

. Cleaning assessment of disinfectant cleaning wipes on an external surface of a medical device contaminated with artificial blood or Streptococcus pneumoniae. Am J Infect Contr 2013; 41: 901–907.

17.

Boyce

Sullivan

Booker

et al.

Quaternary ammonium disinfectant issues encountered in an environmental services department. Infect Contr Hosp Epidemiol 2016; 37: 340–342.

18.

Sawhney

Allen

Reynolds

et al.

Effect of water pressure on absorbency of hydroentangled greige cotton non-woven fabrics. Text Res J 2012; 82: 21–26.

19.

Slopek

Condon

Sawhney

et al.

Adsorption of alkyl-dimethyl-benzyl-ammonium chloride on differently pretreated non-woven cotton substrates. Text Res J 2011; 81: 1617–1624.

20.

Hinchliffe

Condon

Slopek

et al.

The adsorption of alkyl-dimethyl-benzyl-ammonium chloride onto cotton nonwoven hydroentangled substrates at the solid–liquid interface is minimized by additive chemistries. Text Res J 2017; 87: 70–80.

21.

Engelbrecht

Ambrose

Sifuentes

et al.

Decreased activity of commercially available disinfectants containing quaternary ammonium compounds when exposed to cotton towels. Am J Infect Contr 2013; 41: 908–911.

22.

Sanford

. Disinfectants that don't. Ann Intern Med 1970; 72: 282–283.

23.

Tiwari

Ray

Jost

et al.

Forty years of disinfectant failure: outbreak of postinjection Mycobacterium abscessus infection caused by contamination of benzalkonium chloride. Clin Infect Dis 2003; 36: 954–962.

24.

Nam

Slopek

Condon

et al.

Effect of polyester blends in hydroentangled raw and bleached cotton nonwoven fabrics on the adsorption of alkyl-dimethyl-benzyl-ammonium chloride. Text Res J 2015; 85: 1221–1233.

25.

Slopek

Condon

Sawhney

APS

et al.

Effect of cotton pectin content and bioscouring on alkyl-dimethyl-benzyl-ammonium chloride adsorption. Text Res J 2012; 82: 1743–1750.

26.

Ubukata

Nonoguchi

Matsuhashi

et al.

Expression and inducibility in Staphylococcus aureus of the mecA gene, which encodes a methicillin-resistant S. aureus-specific penicillin-binding protein. J Bacteriol 1989; 171: 2882–2885.

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