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Abstract

The explicit form of the kinetic equation for the enzymatic hydrolysis of cellulose fibers with SF enzyme is derived. This equation is effective for estimating and controlling hydrolysis reactions of cellulose with cellulase. With this equation, it is possible to calculate the maximum digestibility and the half maximum digestion constant. Under the same experimental conditions, the maximum digestibility of fibers treated with SF cellu lase decreases in the order viscose rayon > cotton > flax, whereas the concentration of cellulase needed to digest the same fraction of hydrolyzed cellulose decreases in the order flax > cotton > viscose rayon. The enzymatic hydrolysis rate of fibers is greatly affected by cellulase concentration. The results show that the rate is proportional to cellulase concentration at a lower level, while at too high a concentration, the rate increases very little with increased concentration. Thus, it is not rational to select too high a cellulase concentration for the process.

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Kinetics of the Cellulase Catalyzed Hydrolysis of Cellulose Fibers

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