Differential organic DNA extraction of semen sample contaminated with blood for the identification of a serial sexual offender: A case report

Abstract

In many cases of sexual assault, traces of semen are left behind on the victim’s body, clothes and the area in which the assault has taken place. The positive identification of semen is instrumental in supporting such cases. There are several methods of forensic examination of semen reported in literature, but the presence of blood complicates the identification of semen stains. This paper presents one such case study where the presence of blood makes DNA profiling more challenging as the PCR amplification becomes complicated, and the absolute differential isolation is the only way to get the clear profile using identifiler kits.

Keywords

Semen stains forensic examination DNA profiling differential isolation PCR

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References

Green

Clinical forensic medicine: sexual assault and semen persistence. In: Siegel

(ed). Encyclopedia of forensic sciences, Oxford: Elsevier, 2000, pp. 397–403.

Schrader

Schielke

Ellerbroek

et al.

PCR inhibitors – occurrence, properties and removal. J Appl Microbiol 2012; 113: 1014–1026.

Ross

Nelson

Holden

. DNA isolation from small tissue samples using salt and spermine. Nucleic Acids Res 1991; 19: 6053.

Kumar

Gaur

Singh

et al.

Studies of different clue materials for semen detection and DNA fingerprinting in sexual assault cases. Int J Multidiscip Res Dev 2015; 2: 286–288.

Alaeddini

. Forensic implications of PCR inhibition – a review. Forensic Sci Int Genet 2012; 6: 297–305.

Trombley Hall

McKay Zovanyi

Christensen

et al.

Evaluation of inhibitor-resistant real-time PCR methods for diagnostics in clinical and environmental samples. PLoS ONE 2013; 8: e73845.

Smith

Osborn

. Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology. FEMS Microbiol Ecol 2009; 67: 6–20.

Rothe

Roewer

Nagy

. Individual specific extraction of DNA from male mixtures – first evaluation studies. Forensic Sci Int Genet 2011; 5: 117–121.

Differential organic DNA extraction, https://dfs.dc.gov/site (accessed 20 November 2017).

10.

Yoshida

Sekiguchi

Mizuno

et al.

The modified method of two-step differential extraction of sperm and vaginal epithelial cell DNA from vaginal fluid mixed with semen. Forensic Sci Int 1995; 72: 25–33.

11.

Moore

Awusabo-Asare

Madise

et al.

Coerced first sex among adolescent girls in Sub-Saharan Africa: prevalence and context. Afr J Reprod Health 2007; 11: 62.

12.

Lalor

McElvaney

. Child sexual abuse, links to later sexual exploitation/high-risk sexual behavior, and prevention/treatment programs. Trauma Violence Abuse 2010; 11: 159–177.

13.

Ezeh

Abamara

Ndukaihe

et al.

Sexual abuse of children in Awka, Anambra State Nigeria. Res Human Soc Sci 2013; 3: 58–62.