This paper describes a simple procedure for the simultaneous detection of FUC and PGM1 in semen, seminal stains, dental pulps and hair roots after separation by isoelectric focusing using Ampholine pH 5–7. The gel plate was first stained for FUC using an agarose overlay with a buffer of pH 7.5. After reading the FUC types, the FUC overlay was removed and replaced with an agarose overlay for PGM. The results show that the method is useful in medicolegal individualization of seminal stains and teeth when only small samples have to be examined.
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References
1.
BarkJ. E.HarrisM. J.FirthM. (1976) Typing of the common phosphoglucomutase variants using isoelectric focusing — a new interpretation of the phosphoglucomutase system. J. Forens. Sci. Soc.16, 115–20.
2.
BurgessR. M.SuttonJ. G.WhiteheadP. H. (1979) An improved means of enzyme typing of hair roots using isoelectric focusing. J. Forens. Sci.24, 392–6.
3.
KidoA.KomatsuN.OyaM. (1986) δ-L-Fucosidase phenotyping in human placentae, semen and seminal stains. For. Sci. Int.30, 37–43.
4.
KidoA.KomatsuN.OyaM. (1987) δ-L-Fucosidase phenotyping in human tissues, dental pulps and hair roots. For. Sci. Int.33, 53–9.
5.
KühnlP.SpielmannW. (1981) Neuere Entwicklung der Etektrofokussierungstechnik. Ärztl. Lab.27, 255–60.
6.
PetersenN.HeideK. (1974) Nachweis von genetischen Markmalen in der Zahnpulpa. Arch. Kriminol.153, 106–10.
7.
SpencerN.HopkinsonD.A.HarrisH. (1964) Phosphoglucomutase polymorphism in man. Nature204, 742–5.
8.
SuttonJ.G. (1979) Further alleles of phospho-ghioomutase in human semen detected by isoelectric focusing. J. Forens. Sci.24, 189–92.
9.
TurnerB. M.TurnerV. S.BeratisN. G.HirschhornK. (1975) Polymorphism of human α fucosidase. Am. J. Hum. Genet.27, 651–61.
10.
Tutsch-BauerE.OyaM.TrögerH.D. (1981) PGM1-Fokussierung an frischen menschlichen Körpergeweben und nach Lagerung. Referate der 9. Internationalen Tagung der Gesellschaft für forensische Blutgruppenkunde, 155–60.
