Identification of Spermatozoa through Fluorescent Microscopy

Abstract

Ten fluorochromes and one ordinary double stain (for contrast) have been used to stain spermatozoa, both fresh and old, out of which Coriphosphin O yields the best result. When counterstained with eosin, it exhibits a very distinctive, characteristic F-body which can open a new avenue in the cytological differentiation of sex. For medico-legal examination of semen it can also clear confusion in identifying intact, degenerated or disintegrated spermatozoa from similar microparticles which may not be possible with ordinary stains.