Hamsters and rats exposed to single doses of CS were retained for up to 32 months, together with appropriate controls. Survival was unaffected and in no case was the incidence of histological changes significantly higher in the test animals than corresponding control animals. The overall incidence of neoplastic disease was similar in test and control animals. A high incidence of pituitary acidophil adenomas was detected in the female rats: these tumours were present both in control and test rats and it was concluded that they were probably the result of ad libitum feeding.
It was concluded that single doses of CS of 28 800 mg min m−3 administered during 1 hour or 18 000 mg min m−3 administered over 2 hours produced no long-term adverse effects in the two species studied.
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References
1.
BallantyneB. (1977) Riot control agents In: Medical Annual. Bristol, John Wright, p. 7.
2.
BallantyneB.CallawayS. (1972) Inhalation toxicology and pathology of animals exposed to o-chlorobenzylidene malononitrileMed. Sci. Law12, 43.
3.
BallantyneB.SwanstonD. W. (1978) The comparative acute mammalian toxicity of 1-chloroacetophenone (CN) and 2-chlorobenzylidene malononitrile (CS)Arch. Toxicol.40, 75.
4.
BeswickF. W.HollandP.KempK. H. (1972) Acute effects of exposure to orthochlorobenzylidene malononitrile (CS) and the development of toleranceBrit. J. Industr. Med.27, 298.
5.
CarletonH. M.DruryR. A. B. (1957) Histological Technique. 3rd Ed.London, Oxford University Press, p. 186.
6.
CullingC. F. A. (1957) Handbook of Histopaihological Technique. London, Butterworth and Co Ltd., p. 296.
7.
DaenikenA.FriedrichU.LutzW. K.SchlatterC. (1980) Tests for mutagenicity and in vivo DNA binding of o-chlorobenzylidene malononitrile (CS), a riot control agentToxicol. Lett. Special Issue. 1, 247.
8.
DawsonA. B.FriedgoodH. B. (1938) Differentiation of two classes of acidophils in the anterior pituitary of the female rabbit and catStain Technol. 13, 17.
9.
DruryR. A. B.WallingtonE. A. (1967) Carleton's Histological Technique. 4th Ed.London, Oxford University Press, p. 43.
10.
FeronV. J.GriceH. C.GriesemerR.PetoR. (1980) Basic requirements for long-term assays for carcinogenicity In: Long-term and short-term screening assays for carcinogens: a critical appraisal. Lyons, International Agency for Research on Cancer.
11.
FurthJ.NakaneP.PasteelsJ. L. (1976) Tumours of the pituitary gland In: Pathology of Tumours of Laboratory Animals. Vol I, The Rat. Part II. Lyons, International Agency for Research on Cancer, p. 201.
12.
GreavesP.RabemampianinaY. (1982) Choice of rat strain: A comparison of the general pathology and tumour incidence in 2 year old Sprague-Dawley and Long-Evans ratsArch. Toxicol.Suppl 5, 298.
13.
Himsworth report (1971) Report on an enquiry into the medical and toxicological aspects of CS (orthochlorobenzylidene malononitrile) Part II. Command No. 4775. London, Edinburgh, Belfast, HMSO.
14.
HoarR. M. (1976) Toxicology and Teratology In: (Ed.) WagnerJ. E.ManningP. J.The Biology of the Guinea-Pig. New York, Academic Press, p. 269.
15.
MarrsT. C.ColgraveH. F.CrossN. L.GazzardM. F.BrownR. F. R. (1983) A repeated dose study of the toxicity in inhaled 2-chlorobenzylidene malononitrile (CS) aerosol in three species of laboratory animalArch. Toxicol.52, 183–98.
16.
ParkS.GiammonaS. T. (1972) Toxic effects of tear gas on an infant following prolonged exposureAmer. J. Dis. Childh.123, 245.
17.
RossM.BrasG.RagbeerM. (1970) Influence of protein and caloric intake upon spontaneous tumor incidence of the anterior pituitary gland of the ratJ. Nutr.100, 177.
18.
SiegelS. (1956) Son-parametric statistics for the behavioral sciences. New York, McGraw-Hill Inc. p. 109.
19.
UpshallD. G. (1973) Effects of o-chlorobenzylidene malononitrile (CS) and the stress of aerosol inhalation upon rat and embryonic developmentToxicol. Appl. Pharmacol.24, 45.
20.
WHO (1970) Health aspects of chemical and biological weapons. Report of a WHO group of consultants. Geneva. WHO.
