A relatively simple method for differentiating ante-mortem from post-mortem injuries is described. It involves the demonstration of an increase in free histamine at sites of injury by fluorescent microscopy on the basis that release of free histamine is a very early vital reaction to injury.
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References
1.
BirkeG.DunerH.LiljedahlS. O.PernowB.TrowellL. (1958) Histamines, catecholamines and adrenocortical steroids in burns. Acta Chir. Scand.114, 87.
2.
ChakravartyN. (1978) Handbook of Experimental Pharmacology—Histamine II and Anti-histamine, Vol. XVIII/2. Ed. RocheM.SilvaBerlin, Springer-Verlag, p. 98.
3.
FattehA. (1966) Distinction between ante-mortem and post-mortem wounds. J. Forensic Sci. 11, 17.
4.
FazekasI. G.Viragos-KisE. (1965) Der Gehalt Der Erhängongfurche An Freim Histamin Als Vitale Reaktion. Dtsch. Z. Ges Gerichtl. Med.56, 250–260.
5.
FazekasG. I.KosaF.Viragos-KisE.BaschA. (1973) Free and total histamine content of skin in experimental burn injuries. Z. Rechtsmedizm.72, 203.
6.
HakansonR.JuhlinL.OwmanC.SpoirangB. (1970) J. Histochem. and Cytochem. 18, 93–98.
7.
JuhlinL.ShelleyW. B. (1966) Detection of histamine by a new fluorescent o-phthalaldehyde stain. J. Histochem. Cytochem.14, 525–528.
8.
RaekallioJ.MakinenP. L. (1966) Histamine content as a vital reaction—experimental studies. Zacchia. 41, 273–284.
9.
RaekallioJ.MakinenP. L. (1970) Histamine content as a vital reaction—autopsy studies. Zacchia. 45, 403–414.
10.
RaekallioJ. (1973) Estimation of age of injuries by histochemical and biochemical methods. Z. Rechtsmedizm73, 83–102.
11.
ShoreP. A.BurhalterA.CohnV. H.Jr (1959) A method for fluorimetric assay of histamine in tissues. J. Pharmacol. Exp. Ther.127, 182–196.