Restricted accessResearch articleFirst published online 2023-10
Carbon (δ 13 C) and nitrogen (δ 15 N) isotope ratios reveal geographic affinity and dietary status of Ajnala skeletal remains: A forensic anthropological study
Stable isotope analysis from bones and teeth has been widely used to estimate the likely geographic locations and dietary status of individuals whose osseous remains have been retrieved from some forensic or bio-archaeological contexts. The carbon and nitrogen stable isotope signatures can provide insights into geographic affinity and dietary habits. Ajnala skeletal remains represent a serious crime against humanity committed in past by colonial rulers and by amateur archaeologists of modern times. In present study, isotopic concentrations of carbon (δ13C) and nitrogen (δ15N) estimated from 21 mandibular molars have been used to estimate the local or non-local status of badly damaged skeletal remains retrieved from an abandoned well at Ajnala (India). The collagen samples having C/N ratio within range of 2.8–3.6 were considered as well-preserved and non-contaminated ones. The isotope concentrations of carbon and nitrogen varied from −18.7‰ to −22.9‰ and +7.6‰ to +11.7‰, with an average of −20.49 ± 1.2‰ and +9.31 ± 1.1‰, respectively. The analysis of the obtained isotope values reflected the consumption of C3/C4 mixed diet by majority of the individuals, and such type of dietary habits are mainly restricted to the reported Indo-Gangatic plain of India to which slain soldiers reportedly belonged to. These observations corroborated the previous observations about the geographic affinity and dietary status of Ajnala individuals. Though C and N isotopes are by and large not the confirmed/direct indicators of geographic origin, they can provide corroboratory information to support other observations narrowing down the dietary habits of individuals of certain specific geographical regions.
WestJBBowenGJCerlingTE,et al.Stable isotopes as one of nature’s ecological recorders. Trends Ecol Evol2006; 21: 408–414.
2.
Meier-AugensteinWKempHF. Stable isotope analysis: bones and teeth. In: JamiesonAMoenssensAA (eds) Wiley encyclopaedia of forensic science. 2nd ed. Portsmouth, UK: John Wiley’s and Sons, 2012, pp.1–15.
3.
ErikssonG. Stable isotope analysis of human and faunal remains from zvejnieki. In: LarssonLZagorskaI (eds) Back to the origin: new research in the mesolithic–neolithic zvejnieki cemetery and environment, Northern Latvia. Almqvist, Stockholm: Wiksell, 2006, pp.183–215.
4.
BartelinkEJMacKinnonATPrince-BuitenhuysJR,et al.Stable isotope forensics as an investigative tool in missing persons. In: MorewitzSJCollsCS (eds) Handbook of missing persons. Investigations. Switzerland: Springer International Publishing, 2016, pp.443–462.
5.
BartelinkEJBergGEChessonLA, et al.Applications of stable isotope forensics for geolocating unidentified human remains from past conflict situations and large-scale humanitarian efforts. In: LathamKEBartelinkEJFinneganM (eds) New perspectives in forensic human skeletal identification. Academic Press, San Diego (CA): Elsevier, 2018, pp.175–184.
6.
BartelinkEJChessonLA. Recent applications of isotope analysis to forensic anthropology. Forensic Sci Res2019; 4: 29–44.
7.
BirdMICrabtreeSAHaigJ,et al.A global carbon and nitrogen isotope perspective on modern and ancient human diet. Proc Natl Acad Sci U S A2021; 118: e2024642118.
8.
Chesson LA, Meier Augenstein W, Berg GE, et al. Basic principles of stable isotope analysis in humanitarian forensic science. In: Parra RC, Zapico SC and Doughlas DH (eds) Forensic science humanitarian action: interacting with the dead and living. 1st ed. Hoboken NJ: John Wiley and Sons, 2020, pp.285–310.https://doi.org/10.1002/9781119482062.CH20
9.
Katzenberg MA and Waters-Rist AL. Stable isotope analysis. In: Katzenberg MA and Grauer AL (eds) Biological anthropology of the human skeleton, 3rd ed. Wiley-Blackwell, 2018, pp. 467–504.https://doi.org/10.1002/9781119151647.cH14.
10.
BocherensHDruckerDG. ‘Stable isotopes in terrestrial teeth and bones. In: EliasS (ed) Encyclopaedia of quaternary science. 2nd ed., 1. Amsterdam: Elsevier, 2013, pp.304–314.
11.
PostDM. Using stable isotopes to estimate trophic position: models, methods and assumptions. Ecology2002; 83: 703–718.
12.
FryB. Stable isotope ecology. New York NY: Springer, 2008.
13.
BartelinkEJBerryRChessonLA. Stable isotopes and human provenancing. In: MallettXBlytheTBerryR (eds) Advances in forensic human identification. Boca Raton, FL: CRC Press, 2014, pp.165–192.
14.
GreenwaldABurnsGEerkensJW,et al.A multi-scalar reassessment of dietary isotopic variability in bioarchaeology: differentiating secular and inter-individual variation. J Archaeological Sci2022; 141: 105592.
15.
India Meteorological Department, Ministry of Earth Sciences, Govt. of India. https://mausam.imd.gov.in
16.
SehrawatJSPathakRKKaurJ. Human remains from Ajnala, India, 2014. Bioarchaeol Near East2016; 10: 82–90.
17.
CooperF. The crisis in the Punjab, from the 10th of May until the Fall of Delhi. Smith, Elder. 1858.
BatesCCarterM. Mutiny at the margins: new perspectives on the Indian uprising of 1857: documents of the Indian uprising. Vol. 7. New Delhi: Sage Publications, 2017.
20.
SehrawatJSRaiNMeier-AugensteinW. Identification of Ajnala skeletal remains using multiple forensic anthropological methods and techniques: a bioarchaeological report. J Archaeol Sci Rep2020; 32: 102434.
21.
EhleringerJRThompsonAHPodlesakDW, et al.A framework for the incorporation of isotopes and isoscapes in geospatial forensic investigations. In: WestJBBowenGJDawsonTE (eds) Isoscapes: understanding movement, pattern, and process on earth through isotope mapping. Dordrecht, Netherlands: Springer Netherlands, 2010, pp.357–387.
Meier-AugensteinWFraserI. Forensic isotope analysis leads to identification of a mutilated murder victim. Sci Justice2008; 48: 153–159.
24.
BirdMIHaigJUlmS,et al.A carbon and nitrogen isotope perspective on ancient human diet in the British Isles. J Archaeol Sci2022; 137: 105516.
25.
Meier-AugensteinW. Stable isotope forensics: methods and forensic applications of stable isotope analysis. 2nd ed.Hoboken, NJ: Wiley, 2018.
26.
SealyJ. Body tissue chemistry and palaeodiet. In: BrothwellDPollardA (eds) Handbook of archaeological science. Chichester, UK: John Wiley & Sons Ltd, 2005, pp.269–279.
27.
HedgesREMReynardLM. Nitrogen isotopes and the trophic level of humans in archaeology. J Archaeol Sci2007; 34: 1240–1251.
28.
BirdMICrabtreeSAHaigJ,et al.A global carbon and nitrogen isotope perspective on modern and ancient human diet. Proc Natl Acad Sci U S A2021; 118: e2024642118.
29.
BoudinMBoeckxPVandenabeeleP,et al.Improved radiocarbon dating for contaminated archaeological bone collagen, silk, wool and hair samples via cross-flow nanofiltrated amino acids. Rapid Commun Mass Spectrom2013; 27: 2039–2050.
30.
AmbroseSH. Preparation and characterization of bone and tooth collagen for isotopic analysis. J Archaeol Sci1990; 17: 431–451.
31.
CoplenTB. Guidelines and recommended terms for expression of stable-isotope-ratio and gas-ratio measurement results. Rapid Commun Mass Spectrom2011; 25: 2538–2560.
32.
PalincaşN. Radiocarbon dating in archaeology: interdisciplinary aspects and consequences (an overview). In: AIP Conference Proceedings, 2017, 1852:60006. AIP Publishing LLC.
33.
SchoellerDAMinagawaMSlaterR,et al.Stable isotopes of carbon, nitrogen and hydrogen in the contemporary North American human food web. Ecol Food Nutr1986; 18: 159–170.
34.
BurtNGreenwaldAM. Isotopic reconstruction of ancient human diet and health: implications for evolutionary medicine. Palaeopathol Evol Med: Integr Approach2022; 79–94. https://doi.org/10.1093/oso/9780198849711.003.005
35.
SchmittJSchneiderRElsigJ, et al. ‘Carbon isotope constraints on the deglacial CO2 rise from ice cores’. Science (New York, N.Y.)2012; 336: 711–714.
36.
ZuckermanMKArmelagosGJ. The origins of biocultural dimensions in bioarchaeology. In: AgarwalSCGlencrossBA (eds) Social bioarchaeology. Chichester, UK: Wiley-Blackwell, 2011, pp.15–43.
37.
FujithaHAdachiH. Paleohealth based on dental pathology and cribra orbitalia from the ancient Egyptian settlement of qaut. Anthropol Sci2017; 125: 35–42.
38.
NardotoGBSilvaSKendallC, et al.Geographical patterns of human diet derived from stable-isotope analysis of fingernails. Am J Phys Anthropol2006; 131: 137–146.
39.
HedgesREM. On bone collagen—apatite-carbonate isotopic relationships. Int J Osteoarchaeol2003; 13: 66–79.
40.
SealyJJohnsonMRichardsM,et al.Comparison of two methods of extracting bone collagen for stable carbon and nitrogen isotope analysis: comparing whole bone demineralization with gelatinization and ultrafiltration. J Archaeol Sci2014; 47: 64–69.
41.
MinagawaMWadaE. Stepwise enrichment of 15N along food chains: further evidence and the relation between Δ15N and animal age. Geochim Cosmochim Acta1984; 48: 1135–1140.
42.
CheungCJingZTangJ,et al.Diets, social roles, and geographical origins of sacrificial victims at the royal cemetery at Yinxu, Shang China: new evidence from stable carbon, nitrogen, and sulfur isotope analysis. J Anthropol Archaeol2017; 48: 28–45.
43.
BrittonKMcManus-FryECameronA, et al.Isotopes and new norms: investigating the emergence of early modern UK breastfeeding practices at St. Nicholas Kirk, Aberdeen. Int J Osteoarchaeol2018; 28: 510–522.
44.
O’LearyMH. Carbon isotopes in photosynthesis. Bioscience1988; 38: 328–336.
45.
FarquharGDEhleringerJRHubickKT. Carbon isotope discrimination and photosynthesis. Annu Rev Plant Physiol Plant Mol Biol1989; 40: 503–537.
46.
CerlingTEHarrisJMMacFaddenBJ,et al.Global vegetation change through the miocene/pliocene boundary. Nature1997; 389: 153–158.
47.
HartmanG. Are elevated Δ15N values in herbivores in hot and arid environments caused by diet or animal physiology?Funct Ecol2011; 25: 122–131.
48.
FiorentinoGCaracutaVCasielloG,et al.Studying ancient crop provenance: implications from Δ13C and Δ15N values of charred barley in a middle bronze age silo at ebla (NW Syria). Rapid Commun Mass Spectrom2012; 26: 327–335.
49.
BogaardAHeatonTHEPoultonP,et al.The impact of manuring on nitrogen isotope ratios in cereals: archaeological implications for reconstruction of diet and crop management practices. J Archaeol Sci2007; 34: 335–343.
50.
AguileraMArausJLVoltasJ, et al.Stable carbon and nitrogen isotopes and quality traits of fossil cereal grains provide clues on sustainability at the beginnings of mediterranean agriculture. Rapid Commun Mass Spectrom: Int J Devoted Rapid Dissemination Minute Res Mass Spectrom2008; 22: 1653–1663.
51.
SchoeningerMJMooreK. Bone stable isotope studies in archaeology. J World Prehistory1992; 6: 247–296.
52.
AlkassKSaitohHBuchholzBA,et al.Analysis of radiocarbon, stable isotopes and DNA in teeth to facilitate identification of unknown decedents. PLoS One2013; 8: e69597.
53.
ChenX-XYuS-YUnderhillAP,et al.Radiocarbon dating and stable carbon isotopic analyses of neolithic and bronze age staple crops in the lower yellow river area and their paleodietary implications. Geoarchaeology2018; 33: 307–313.
54.
SaudTGautamRMandalTK,et al.Emission estimates of organic and elemental carbon from household biomass fuel used over the Indo-Gangetic plain (IGP), India. Atmos Environ2012; 61: 212–220.
55.
SharmaSKMandalTKShenoyDM, et al.Variation of stable carbon and nitrogen isotopic composition of PM10 at urban sites of Indo Gangetic plain (IGP) of India. Bull Environ Contam Toxicol2015; 95: 661–669.
56.
SehrawatJSSinghM. Dental health status of skeletal remains excavated from an abandoned well at Ajnala, India: a bioarchaeological study. Anthropol Rev2018; 81: 307–324.
57.
SehrawatJSSinghM. Forensic odontological sex determination of Ajnala skeletal remains based on the statistical equations generated from the odontometrics of known teeth. Forensic Sci Int: Rep2019; 1: 100044.
58.
SehrawatJSSinghM. Application of trace elemental profile of known teeth for sex and age estimation of Ajnala skeletal remains: a forensic anthropological cross-validation study. Biol Trace Elem Res2020a; 193: 295–310.
59.
SehrawatJS. Dental age estimation of Ajnala skeletal remains: a forensic odontological study. Bull Int Assoc Paleodontol2020; 14: 40–52.
60.
SehrawatJSSinghM. Application of Kvaal’s radiological method for dental age estimation of Ajnala skeletal remains: a cross-validation study. Forensic Imaging2020b; 22: 200401.
61.
MakarewiczCASealyJ. Dietary reconstruction, mobility, and the analysis of ancient skeletal tissues: expanding the prospects of stable isotope research in archaeology. J Archaeol Sci2015; 56: 146–158.
62.
SehrawatJSSankhyanDDuttS,et al.‘Stable C and N isotope analysis of Human Dental Remains retrieved from an Ancient Well at Ajnala (Punjab, India): assessment of dietary status and geographic affiliations’. https://doi.org/10.21203/rs.3.rs-1053629/v1. 2021.
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