Cumulative Correlations of Lysozyme,Lactoferrin,Peroxidase,S-IgA,Amylase,and Total Protein Concentrations with Adherence of Oral Viridans Streptococci to Microplates Coated with Human Saliva
Restricted accessResearch articleFirst published online March, 1999
Cumulative Correlations of Lysozyme,Lactoferrin,Peroxidase,S-IgA,Amylase,and Total Protein Concentrations with Adherence of Oral Viridans Streptococci to Microplates Coated with Human Saliva
Redundancy refers to the observation that many salivary proteins exhibit similar properties in vitro. It is possible that bacterial adherence to salivary pellicle occurs as a cumulative effect of multiple proteins. This study determined the joint and individual contributions of salivary amylase, S-IgA, lysozyme, salivary peroxidase, lactoferrin, and total protein concentrations to adherence by oral viridans streptococci in microplates coated with whole saliva from 123 persons. Strains used were: Streptococcus gordonii Blackburn, 10558, Streptococcus mitis 10712, 903, Streptococcus oralis 10557, 9811, and Streptococcus sanguis 10556, 13379. Rabbit antibody against 13379 was used for the detection of adherence. This antibody cross-reacted with all strains. Absorbance was standardized against saliva pooled from five donors. All saliva samples had been previously assayed for amylase, lactoferrin, lysozyme, secretory IgA, peroxidase, and total protein. Adherence scores for all strains except 13379 were significantly and positively correlated. Salivas binding high or low levels of one strain tended to bind others correspondingly. Multiple regression indicated significant contributions to 10558 adherence from total protein and lactoferrin (positive), and peroxidase and lysozyme (negative). Similar results were obtained for Blackburn and 903. Significant individual correlations were seen for 9811 and total protein (positive), 10557 and peroxidase (negative), and 13379 and lactoferrin (negative). Salivas with high adherence scores contained significantly more protein and lactoferrin, and significantly less peroxidase, than salivas with low adherence scores. These findings support the hypothesis that multiple proteins contribute to the adherence of streptococcal strains in vivo.
Al-Hashimi I., Levine MJ (1989). Characterization of in vivo salivary-derived enamel pellicle. Arch Oral Biol34:289-295.
2.
Amano A., Sharma A., Lee JY, Sojar HT, Raj PA, Genco RJ (1996). Structural domains of Porphyromonas gingivalis recombinant fimbrillin that mediate binding to salivary proline-rich protein and statherin. Infect Immun64:1631-1637.
3.
Brady LJ, Piacentini DA, Crowley PJ, Oyston PC, Bleiweis AS (1992). Differentiation of salivary agglutinin-mediated adherence and aggregation of mutans streptococci by use of monoclonal antibodies against the major surface adhesin P1. Infect Immun60:1008-1017.
4.
Brooks W., Demuth DR, Gil S., Lamont RJ (1997). Identification of a Streptococcus gordonii SspB domain that mediates adhesion to Porphyromonas gingivalis. Infect Immun65:3753-3758.
5.
Cantarero LA, Butler JE, Osborne JW (1980). The adsorptive characteristics of proteins for polystyrene and their significance in solid-phase immunoassays. Anal Biochem105:375-382.
6.
Cvitkovitch DG, Hamilton IR (1994). Biochemical change exhibited by oral streptococci resulting from laboratory subculturing. Oral Microbiol Immunol9:209-217.
7.
Demuth DR, Duan Y., Brooks W., Holmes AR, McNab R., Jenkinson HF (1996). Tandem genes encode cell-surface polypeptides SspA and SspB which mediate adhesion of the oral bacterium Streptococcus gordonii to human and bacterial receptors. Molec Microbiol20:403-413.
8.
Frandsen EV, Pedrazzoli V., Kilian M. (1991). Ecology of viridans streptococci in the oral cavity and pharynx. Oral Microbiol Immunol6:129-133.
9.
Germaine GR, Tellefson LM (1986). Potential role of lysozyme in bactericidal activity of in vitro-acquired salivary pellicle against Streptococcus faecium 9790 . Infect Immun54:846-854.
10.
Gibbons RJ, Etherden I. (1985). Albumin as a blocking agent in studies of streptococcal adsorption to experimental salivary pellicles. Infect Immun50:592-594.
11.
Gibbons RJ, Hay DI, Cisar JO, Clark WB (1988). Adsorbed salivary proline-rich protein 1 and statherin: receptors for type 1 fimbriae of Actinomyces viscosus T14V-J1 on apatitic surfaces. Infect Immun56:2990-2993.
12.
Gibbons RJ, Hay DI, Schlesinger DH (1991). Delineation of a segment of adsorbed salivary acidic proline-rich proteins which promotes adhesion of Streptococcus gordonii to apatitic surfaces. Infect Immun59:2948-2954.
13.
Gong K., Herzberg MC (1997). Streptococcus sanguis expresses a 150-kilodalton two-domain adhesin: characterization of several independent adhesin epitopes . Infect Immun65:3815-3821.
14.
Gong K., Wen D.-Y, Ouyang T., Rao AT, Herzberg MC (1995). Platelet receptors for the Streptococcus sanguis adhesin and aggregation-associated antigens are distinguished by antiidiotypical monoclonal antibodies. Infect Immun63 :3628-3633.
Lassiter MO, Newsome AL, Sams LD, Arnold RR ( 1987). Characterization of lactoferrin interaction with Streptococcus mutans. J Dent Res66:480-485.
17.
Levine MJ (1993). Development of artificial salivas. Crit Rev Oral Biol Med4:279-286.
18.
Ligtenberg AJ, Walgreen-Weterings E., Veerman EC, de Graaff J., Nieuw Amerongen AV (1993). Adherence of Streptococcus gordonii HG 222 in the presence of saliva. Antonie van Leeuwenhoek64:39-45.
19.
Ligtenberg AJM, Walgreen-Weterings E., Veerman EC, de Soet JJ, de Graaff J., Nieuw Amerongen AV (1992). Influence of saliva on aggregation and adherence of Streptococcus gordonii HG 222. Infect Immun60:3878-3884.
20.
Liljemark WF, Bloomquist CG, Ofstehage JC (1979). Aggregation and adherence of Streptococcus sanguis: role of human salivary immunoglobulin A. Infect Immun26:1104-1110.
21.
Lowry OH, Rosebrough N., Farr A., Randall R. (1951). Protein measurement with the folin phenol reagent . J Biol Chem193:265-275.
22.
Ma JK, Kelly CG, Munro G., Whiley RA, Lehner T. (1991). Conservation of the gene encoding streptococcal antigen I/II in oral streptococci. Infect Immun59:2686-2694.
23.
Mansson-Rahemtulla B., Baldone DC, Pruitt KM, Rahemtulla F. (1986). Specific assays for peroxidases in human saliva . Arch Oral Biol31:661-668.
24.
McNab R., Holmes AR, Clarke JM, Tannock GW, Jenkinson HF (1996). Cell surface polypeptide CshA mediates binding of Streptococcus gordonii to other oral bacteria and to immobilized fibronectin . Infect Immun64:4204-4210.
25.
Moisset A., Schatz N., Lepoivre Y., Amadio S., Wachsmann D., Scholler M., et al. (1994). Conservation of salivary glycoprotein-interacting and human immunoglobulin G-cross-reactive domains of antigen I/II in oral streptococci. Infect Immun62:184-193.
26.
Motley MA, Arnold RR (1987). Cofactor requirements for expression of lactoferrin bactericidal activity on enteric bacteria. Adv Exp Med Biol216:591-599.
27.
Murray PA, Prakobphol A., Lee T., Hoover CI, Fisher SJ (1992). Adherence of oral streptococci to salivary glycoproteins . Infect Immun60:31-38.
28.
Pollock JJ, Lotardo S., Gavai R., Grossbard BL (1987). Lysozyme protease-inorganic monovalent anion lysis of oral bacteria strains in buffers and stimulated whole saliva. J Dent Res66:467-474.
29.
Roger V., Tenovuo J., Lenander-Lumikari M., Soderling E., Vilja P. (1994). Lysozyme and lactoperoxidase inhibit the adherence of Streptococcus mutans NCTC 10449 (serotype c) to saliva-treated hydroxyapatite in vitro. Caries Res28 :421-428.
30.
Rosan B., Appelbaum B., Golub E., Malamud D., Mandel ID (1982). Enhanced saliva-mediated bacterial aggregation and decreased bacterial adhesion in caries-resistant versus caries-susceptible individuals. Infect Immun38:1056-1059.
31.
Rudney JD (1995). Does variability in salivary protein concentrations influence oral microbial ecology and oral health? Crit Rev Oral Biol Med6:343-367.
32.
Rudney JD, Krig MA, Neuvar EK, Soberay AH, Iverson L. (1991). Antimicrobial proteins in human unstimulated whole saliva in relation to each other, and to measures of health status, dental plaque accumulation and composition. Arch Oral Biol36:497-506.
33.
Rudney JD, Ji Z., Larson CJ (1995a). The prediction of saliva swallowing frequency in humans from estimates of salivary flow rate and the volume of saliva swallowed . Arch Oral Biol40:507-512.
34.
Rudney JD, Ji Z., Larson CJ, Liljemark WF, Hickey KL (1995b). Saliva protein binding to layers of oral streptococci in vitro and in vivo. J Dent Res74:1280-1288.
35.
Rudney JD, Ji Z., Larson CJ (1996). Saliva protein binding to streptococcal layers placed at different oral sites in 48 persons. J Dent Res75:1789-1797.
36.
Rundegren JL, Arnold RR (1987). Bacteria-agglutinating characteristics of secretory IgA and a salivary agglutinin. Adv Exp Med Biol216:1005-1013.
37.
Sampson JS, O'Connor SP, Stinson AR, Tharpe JA, Russell H. (1994). Cloning and nucleotide sequence analysis of psaA, the Streptococcus pneumoniae gene encoding a 37-kilodalton protein homologous to previously reported Streptococcus sp. adhesins. Infect Immun62:319-324.
38.
Scannapieco FA (1994). Saliva-bacterium interactions in oral microbial ecology. Crit Rev Oral Biol Med5:203-248.
39.
Scannapieco FA, Bergey EJ, Reddy MS, Levine MJ (1989). Characterization of salivary alpha-amylase binding to Streptococcus sanguis. Infect Immun57:2853-2863.
40.
Scannapieco FA, Torres GI, Levine MJ (1995). Salivary amylase promotes adhesion of oral streptococci to hydroxyapatite. J Dent Res74:1360-1366.
41.
Schilling KM, Carson RG, Bosko CA, Golikeri G., Bruinooge A., Hoyberg K., et al. (1994). A microassay for bacterial adherence to hydroxyapatite. Colloid Surf B3:31-38.
Soukka T., Roger V., Soderling E., Tenovuo J. (1994). Binding of Streptococcus mutans, serotype c, to saliva-coated hydroxyapatite in the presence and absence of human lactoferrin . Microbial Ecol Health Dis7:139-144.
44.
Tellefson LM, Germaine G. (1986). Adherence of Streptococcus sanguis to hydroxyapatite coated with lysozyme and lysozyme-supplemented saliva. Infect Immun51:750-759.
45.
Tenovuo J., Moldoveanu Z., Mestecky J., Pruitt KM, Rahemtulla BM (1982). Interaction of specific and innate factors of immunity: IgA enhances the antimicrobial effect of the lactoperoxidase system against Streptococcus mutans. J Immunol128:726-731.
46.
Veerman EC, Ligtenberg AJ, Schenkels LC, Walgreen-Weterings E., Nieuw Amerongen AV (1995). Binding of human high-molecular-weight salivary mucins (Mgl) to Hemophilus parainfluenzae. J Dent Res74:351-357.
47.
Wolf R., Taylor L. (1964). A comparative study of saliva protein analysis . Arch Oral Biol9:135-140.
