Localization of Estrogen-receptor-related Antigen in Human Odontoblasts

Abstract

Estrogen receptors have been demonstrated in many osteogenic cell lines. Recently, we showed that estrogen deficiency induced by ovariectomy caused enhanced dentin formation in adult rats, suggesting that estrogen receptors may be present in dental tissues. Nothing is known about estrogen receptors in human teeth. We used immunohistochemical staining and immuno-blotting to demonstrate the presence of estrogen receptors in human pulp and/or the pulpo-dentinal border. Unerupted human wisdom teeth were surgically removed, frozen in liquid nitrogen, and prepared for immunological studies. Western blot analysis with monoclonal antibodies specific for human estrogen-receptor-related antigens demonstrated an approximately 29-kDa clear double band in the material scraped from the predentin-odontoblast border and in the fluid that emerged into the pulpal chamber, evidently from the odontoblasts. A weaker double band was also present in pulpal tissue samples. By immunohistochemical staining, estrogen-receptor-related antigens were visualized in the predentinal-odontoblast region and in the pulpal blood vessels. Our results suggest the presence of estrogen receptors in human teeth, and thus the previously reported enhancement of the dentin formation in rats after ovariectomy may be mediated via these receptors.

Keywords

estrogen receptor pulp odontoblast Western blot

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References

Ben-Hur H. , Mor G. , Blickstein I. , Likham I. , Kohen F. , Dgani R. , et al. (1993). Localization of estrogen receptors in long bones and vertebrae of human fetuses. Calcif Tissue Int 53:91-96.

Brandi ML , Crescioli C. , Tanini A. , Frediani U. , Agnusdei D. , Gennari C. (1993). Bone endothelial cells as estrogen targets. Calcif Tissue Int 53:312-317.

Coffer AI , Lewis KM , Brockas AJ , King RJ ( 1985a). Monoclonal antibodies against a component related to soluble estrogen receptor. Cancer Res 45:3686-3693.

Coffer AI , Spiller GH , Lewis KM , King RJ ( 1985b). Immuno-radiometric studies with monoclonal antibody against a component related to human estrogen receptor. Cancer Res 45:3694-3698.

Eriksen EF , Colvard DS , Berg NJ , Graham ML , Mann KG , Spelsberg TC (1988). Evidence of estrogen receptors in normal human osteoblast-like cells. Science 241:84-86.

Ernst M. , Heath JK , Rodan GA (1989). Estradiol effects on proliferation, messenger ribonucleic acid for collagen and insulin-like growth-factor I, and parathyroid hormone-stimulated adenylate cyclase activity in osteoblastic cells from calvariae and long bones. Endocrinology 125:825-833.

Hietala E-L. , Larmas M. (1992). The effect of ovariectomy on dentin formation and caries in adult rats. Acta Odontol Scand 50:337-343.

Ikegami A. , Inoue S. , Hosoi T. , Mizuno Y. , Nakamura T. , Ouchi Y. , et al. (1993). Immunohistochemical detection and Northern blot analysis of estrogen receptor in osteoblastic cells. J Bone Miner Res 8:1103-1109.

Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 :680-685.

10.

Miller SC , Bowman BM , Miller MA , Bagi CM ( 1991). Calcium absorption and osseous organ-, tissue-, and envelope-specific changes following ovariectomy in rats. Bone 12 :439-446.

11.

Ohashi T. , Kusuhara S. , Ishida K. (1991). Estrogen target cells during the early stages of medullary bone osteogenesis: Immunohistochemical detection of estrogen receptors in osteogenic cells of estrogen-treated male Japanese quail. Calcif Tissue Int 49:124-127.

12.

Pansler JM , Radosevich JA , Higbee R. , Langman CB (1990). Osteoclasts isolated from membranous bone in children exhibit nuclear estrogen and progesterone receptors. J Bone Miner Res 5:797-802.

13.

Svanberg M. , Hietala E-L. , Knuuttila M. (1994). The effect of dietary xylitol on dentin formation in ovariectomized rats. Acta Odontol Scand 52:82-85.

14.

Turner RT , Vandersteenhoven JJ , Bell NH ( 1987). The effects of ovariectomy and 17-estradiol on cortical bone histomorphometry in growing rats. J Bone Miner Res 2:115-122.

15.

Turner RT , Bell NH , Gay CV (1993). Evidence that estrogen binding sites are present in bone cells and mediate medullary bone formation in Japanese quail. Poult Sci 72:728-740.