The abundant hydrophobic, proline-glutamine, and histidine-rich (over 90%) amelogenins constitute the major class of proteins in forming extracellular enamel matrix. These are thought to play a major role in the structural organization and mineralization of developing enamel. The present report describes the successful sequencing of the major human amelogenin protein, by use of both Edman degradation and cDNA sequencing. When Edman degradation was used, over 75% of the primary structure of the protein was determined. This sequence was supplemented with cDNA sequencing studies, which revealed the predicted sequence of this protein. Together, they provide the complete sequence of an important human enamel protein. The information complements recent studies on bovine and human amelogenin genes. A comparison between the present results and the protein sequences predicted from the corresponding human amelogenin genomic coding regions and that of cDNA sequences of other species is described.
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References
1.
Catalano-Sherman J., Burstein Y., Peretz M., Deutsch D. (1990). Sequencing of human amelogenin (abstract). J Dent Res69:1033.
2.
Deutsch D. (1989). Structure and function of enamel gene products . Anat Rec224:189-210.
3.
Deutsch D., AlayoffA. (1987). Changes in amino acid composition and protein distribution during development of human deciduous enamel. Growth51:342-354.
4.
Deutsch D., Shapira L., Alayoff A., Leviel D., Yoeli Z., Arad A. ( 1984). Protein and mineral changes during prenatal and postnatal development and mineralization of human deciduous enamel. In: Fearnhead RW, Suga S , editors. Tooth Enamel IV. Amsterdam : Elsevier Science Publishers, 234-239.
5.
Fincham AG, Ha Y., Pavlova Z., Slavkin HC, Snead ML (1989). Human amelogenins: sequences of "TRAP" molecules . Calcif Tissue Int45:243-250.
6.
Fincham AG, Belcourt AB, Termine JD, Butler WT, Cothran C. (1983). Amelogenins: sequence homologies in enamel-matrix protein from three mammalian species. Biochem J211:149-154.
7.
Fincham AG, Ha Y., Lau E., Pavlova Z., Slavkin HC, Snead ML (1990). Isolation and partial characterization of a human amelogenin from a single fetal dentition using HPLC techniques. Calcif Tissue Int47:105-111.
8.
Gibson C., Golub E., Herold R., Risser M., Ding W., Shimokawa H., et al. (1991). Structure and expression of the bovine amelogenin gene. Biochemistry30:1075-1079.
9.
Glisin V., Crkvenjakov R., Byus C. ( 1974). Ribonucleic acid isolated by cesium chloride centrifugation . Biochemistry13:2633.
10.
Gross E. (1967). The cyanogen bromide reaction. Meth Enzymol11:238-255.
11.
Lagerstrom M., Dahl N., Nakahori Y., Nakagome Y., Backman B., Landergren U., et al. (1991). A deletion in the amelogenin gene (AmG) causes X-linked amelogenesis imperfecta (AIH1). Genomics10:971-975.
12.
Lau EC, Mohandas TD, Shapiro LJ, Slavkin HC, Snead ML (1989). Human and mouse amelogenin loci are on the sex chromosomes. Genomics4:162-168.
13.
Matsudaira P. (1987). Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem262:10035-10038.
14.
Nakahori Y., Takenaka 0, Nakagome Y. (1991). The human X-Y homologous region encodes "amelogenin" . Genomics9:264-269. Ornstein C. (1964). Disc electrophoresis I. Background and theory. Ann NY Acad Sci121:321-348.
15.
Robinson C., Lowe NR, Weatherell JA (1977). Changes in the amino acid composition of developing rat incisor enamel. Calcif Tissue Res23:19-31.
16.
Salido EC, Yen PH, Koprivnikar K., Yu L., Shapiro L. (1992). The human enamel protein gene amelogenin is expressed from both the X and the Y chromosomes. Am J Hum Genet50:303-316.
17.
Shimokawa H., Ogata Y., Sasaki S., Sobel ME, McQuillan CI, Termine JD, et al. (1987). Molecular cloning of bovine amelogenin cDNA. Adv Dent Res1:293-297.
18.
Shimokawa H., Sasaki S. (1978). Study on the biosynthesis of bovine enamel in vitro. J Dent Res57:133-138.
19.
Shimokawa H., Sobel M., Sasaki M., Termine J., Young M. (1986). Heterogeneity of amelogenin mRNA in the bovine tooth. J Biol Chem262:4042-4047.
20.
Shimokawa H., Tamura M., Ibaraki K., Ogata Y., Sasaki S. (1989). Human amelogenin gene. In: Fearnhead RW, editor. Tooth enamel V. Yokohama: Florence Publishers, 301-305.
21.
Slavkin HC, Bessem C., Bringas P., Zeichner-David M., Nanci A., Snead ML (1988). Sequential expression and differential function of multiple enamel proteins during fetal neonatal and early postnatal stage of mouse molar organogenesis. Differentiation37:26-39.
22.
Smyth DG (1967). The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. Meth Enzymol11:214-231.
23.
Snead M., Lau E., Fincham A., Zeichner-David M., Slavkin H. (1989). Knowledge based molecular dissection of amelogenesis: the chromosomal location of the amelogenin genes. In: Fearnhead RW, editor. Tooth enamel V. Yokohama: Florence Publishers, 261-265.
24.
Snead ML, Lau E., Zeichner-David M., Woo LS, Slavkin HC (1985). DNA sequence for cloned cDNA for murine amelogenin reveal the amino acid sequence for enamel specific protein. Res Commun129:3812-3818.
25.
Takagi T., Suzuki M., Baba T., Minegishi K., Sasaki S. (1984). Complete amino acid sequence of amelogenin in developing bovine enamel. Biochem Biophys Res Commun121:592-597.
26.
Termine JD, Belcourt AB, Christner PJ (1980). Properties of dissociatively extracted fetal tooth matrix proteins. I. Principal molecular species in developing bovine enamel . J Biol Chem225:9760-9768.
27.
Weber K., Osborn M. (1969). The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem24:4406-4412.
28.
Winship RP (1989). An improved method for directly sequencing PCR amplified material using DMSO. Acids Res17:1266.
29.
Yamakoshi Y., Tanabe T., Fukae M., Shimizu M. (1989). Amino acid sequence of porcine 25 kDa amelogenin . In: Fearnhead RW, editor. Tooth enamel V. Yokohama: Florence Publishers, 314-318.
