Aberrant expression of TGF-a is associated with human malignant oral epithelium. Experiments were initiated to determine the cellular sources of transforming growth factor-alpha (TGF-a) in human oral cancer. Ten freshly resected human oral cancers and four specimens of normal human oral epithelium were studied by in situ hybridization and immunohistochemistry. Tissues were probed with 35S-labeled sense and antisense riboprobes to (i) human TGF-a (hTGF-a), (ii) human epidermal growth factor receptor (EGFR) to determine the distribution of TGF-a responsive cells, and (iii) histone H3 to examine TGF-a and/or EGFR's possible contribution to altered proliferation in transformed epithelium. Results of our experiments showed that TGF-a mRNA could be detected in normal and transformed human oral epithelium. More surprising, we have identified the major source of TGF-a mRNA to be the infiltrating eosinophils. A monoclonal antibody to the mature human TGF-a peptide stained similar areas in normal and malignant specimens. Eosinophils associated with tumors exhibited positive cytoplasmic immunostaining for TGF-a protein. Labeling of EGFR mRNA in human oral epithelium demonstrated uniform labeling of basal layers in normal, hyperplastic, and mildly dysplastic epithelium. In severely dysplastic epithelium and carcinomas (particularly moderate to poorly differentiated types), cellular levels of EGFR mRNA were significantly higher. The profile of altered cellular levels of EGFR mRNA correlated well with the profile of altered proliferation as indicated by H3 mRNA labeling. We hypothesize that the overproduction of EGFR mRNA in tumor epithelium-together with the localized delivery of high amounts of TGF-a by eosinophils at tumor-developing sites-is responsible for the increased proliferation of the tumor epithelium.
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