The present study describes the detailed changes in carbonate distribution throughout the different stages of development in human and bovine enamel, from early formation to maturation. Twenty-two human maxillary and mandibular deciduous anterior teeth and 46 bovine teeth were studied. The major mineral ions, calcium and phosphorus, were also analyzed to facilitate comparison of carbonate content with progressing mineralization. The results showed that as enamel matured and mineral concentration increased, carbonate concentration decreased. The observed decrease in percent carbonate per weight enamel mineral may be due to dilution by an influx of relatively carbonate-free mineral, and could, in part, explain the observed increase in crystallinity of enamel mineral as the tissue matures.
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References
1.
Beshah, K.; Rey, C.; Glimcher, M.J.; Schimizu, M.; and Griffin, R.G. (1990): Solid State Carbon-13 and Proton NMR Studies of Carbonate Containing Calcium Phosphates and Enamel, J Solid State Chemistry84:71-81.
2.
Chen, P.S.; Toribara, T.Y.; and Warner, H. (1956): Microdetermination of Phosphorus, Anal Chem28:1756-1758.
3.
Coolidge, T.B. and Jacobs, M.H. (1957): Enamel Carbonate in Caries, J Dent Res36:765-768.
4.
Darling, A.I. (1961): The Selective Attack of Caries on Dental Enamel , Ann R Coll Surg29:354-369.
5.
Deakins, M. (1942): Changes in the Ash, Water, and Organic Content of Pig Enamel During Calcification, J Dent Res21:429-435.
6.
Deutsch, D. (1989): Structure and Function of Enamel Gene Products , Anat Rec224:189-210.
7.
Deutsch, D.; El-Attar, I.; Robinson, C.; and Weatherell, J. (1979): Rate and Timing of Enamel Development in the Deciduous Bovine Incisor, Arch Oral Biol24:407-413.
8.
Deutsch, D. and Gedalia, I. (1980): Chemically Distinct Stages in Developing Fetal Human Enamel, Arch Oral Biol25:635-639.
9.
Deutsch, D. and Pe'Er, E. (1982): Development of Enamel in Human Fetal Teeth, J Dent Res61(Sp Iss):1543-1551.
10.
Deutsch, D. and Shapira, L. (1987): Changes in Mineral Distribution and Concentration During Enamel Development in the Deciduous Human Maxillary and Mandibular Teeth, Growth51:334-341.
11.
Deutsch, D.; Shapira, L.; Alayoff, A.; Leviel, D.; Yoeli, Z.; and Arad, A. (1984): Protein and Mineral Changes During Prenatal and Postnatal Development and Mineralization of Human Deciduous Enamel. In: Tooth Enamel IV, R.W. Fearnhead and S. Suga, Eds., Amsterdam: Elsevier Publishing Co., pp. 234-239.
12.
Eastoe, J.E. and Camilleri, G.E. (1971): Pattern of Protein Distribution in Enamel During Maturation. In: Tooth Enamel II, R.W. Feamhead and M.V. Stack, Eds., Bristol: John Wright, pp. 119-123.
13.
Elliott, J.C.; Holcomb, D.W.; and Young, R.A. (1985): Infrared Determination of the Degree of Substitution of Hydroxyl by Carbonate Ions in Human Dental Enamel, Calcif Tissue Int37:372-375.
14.
Featherstone, J.D.B.; Pearson, S.,; and Legeros, R.Z. (1984): An Infrared Method for Quantification of Carbonate in Carbonated Apatites, Caries Res18:63-66.
15.
Hallsworth, A.S.; Robinson, C.; and Weatherell, J.A. (1972): Mineral and Magnesium Distribution Within the Approximal Carious Lesion, Caries Res6:156-168.
16.
Hallsworth, A.S.; Weatherell, J.A.; and Robinson, C. (1973): Loss of Carbonate During the First Stages of Enamel Caries, Caries Res7:343-348.
Legeros, R.Z. and Tung, M.S. (1983): Chemical Stability of Carbonate- and Fluoride-containing Apatites, Caries Res17:419-429.
23.
Mayer, I.; Featherstone, J.D.B.; Nagler, R.; Noejovich, M.; Deutsch, D.; and Gedalia, I. (1985): The Thermal Decomposition of Mgcontaining Carbonate Apatites, J Solid State Chem56:230-235.
24.
Nelson, D.G.A. and Featherstone, J.D.B. (1982): Preparation, Analysis, and Characterization of Carbonated Apatites, Calcif Tissue Int34:S69-S81.
25.
Nylen, M.; Eanes, E.D.; and Omnell, K.A. (1963): Crystal Growth in Rat Enamel, J Cell Biol18:109-123.
26.
Robinson, C.; Fuchs, P.; Deutsch, D.; and Weatherell, J.A. (1978): Four Chemically Distinct Stages in Developing Enamel from Bovine Incisor Teeth, Caries Res12:1-11.
Weatherell, J.A. and Robinson, C. (1968): Micro Determination of Carbonate in Dental Enamel , Analyst93:244-248.
29.
Weatherell, J.A. and Robinson, C. (1973): The Inorganic Composition of Teeth. In: Biological Mineralization, I. Zipkin, Ed., New York: John Wiley and Sons, Inc., pp. 43-74.
30.
Weatherell, J.A.; Robinson, C.; and Hallsworth, A.S. (1974): Variations in the Chemical Composition of Human Enamel, J Dent Res53 :180-192.
31.
Weatherell, J.A.; Robinson, C.; and Hiller, C.R. (1968): Distribution of Carbonate in Thin Sections of Dental Enamel, Caries Res2:1-9.
