Hen-egg-yolk antibody (IgY) was prepared against Streptococcus mutans MT8148 serotype c that was cultivated in medium containing sucrose, and it was used in passive caries-immunity studies. Specific pathogen-free rats infected with S. mutans MT8148 (c) and fed with a cariogenic diet containing more than 2% immune yolk powder developed significantly lower caries scores than did the ones infected with the same strain and fed with a diet containing only control yolk powder obtained from non-immunized hens. Similar results were obtained in an experiment with rats infected with S. mutans JC-2 (c) strain. Rats provided a diet supplemented with 0.5% immune water-soluble protein fraction containing S. mutans-specific IgY and challenged with S. mutans MT8148 exhibited significantly fewer caries lesions, compared with control rats on the normal diet.
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References
1.
Bartz, C.R.; Conklin, R.H.; Tunstall, C.B.; and Steele, J.H. (1980): Prevention of Murine Rotavirus Infection with Chicken Egg Yolk Immunoglobulins, J Infect Dis142:439-441.
2.
Bratthall, D. and Kohler, B. (1976): Streptococcus mutans Serotypes: Some Aspects of their Identification, Distribution, Antigenic Shifts, and Relationship to Caries, J Dent Res55 :C15-C21.
3.
Cost, K.M.; West, C.S.; Brinson, D.; and Polk, H.C., Jr. (1985): Measurement of Human Antibody Activity against Escherichia coli and Pseudomonas aeruginosa using Formalin Treated Whole Organisms in an ELISA Technique, J Immunoassay6:23-43.
4.
Hatta, H.; Kim, M.; and Yamamoto, T. (1990): A Novel Isolation Method for Hen Egg Yolk Antibody, "IgY", Agric Biol Chem54:2531-2535.
5.
Hatta, H.; Sim, J.S.; and Nakai, S. (1988): Separation of Phospholipids from Egg Yolk and Recovery of Water-soluble Proteins, J Food Sci53:425-431.
6.
Heller, E.D. (1975): The Immune Response of Hens to Multiple Escherichia coli Injections and Transfer of Immunoglobulins to the Egg and Hatched Chick , Res Vet Sci18:117-120.
7.
Hughes, M.; MacHardy, S.M.; Sheppard, A.J.; and Woods, N.C. (1980): Evidence for an Immunological Relationship between Streptococcus mutans and Human Cardiac Tissue, Infect Immun27:576-588.
8.
Keyes, P.H. (1958): Dental Caries in the Molar Teeth of Rats: II. A Method for Diagnosing and Scoring Several Types of Lesions Simultaneously , J Dent Res37:1088-1099.
9.
Keyes, P.H. and Jordan, H.V. (1964): Periodontal Lesions in the Syrian Hamster-III: Findings Related to an Infectious and Transmissible Component, Arch Oral Biol9:377-400.
10.
Lehner, T.; Caldwell, J.; and Smith, R. (1985): Local Passive Immunization by Monoclonal Antibodies against Streptococcal Antigen I/II in the Prevention of Dental Caries, Infect Immun50:796-799.
11.
Lehner, T.; Challacombe, S.J.; Russell, M.W.; Scully, C.M.; and Hawkes, J.E. (1978): Passive Immunization with Serum and Immunoglobulins against Dental Caries in Rhesus Monkeys, Lanceti:693-694.
12.
Lehner, T.; Russell, M.W.; Caldwell, J.; and Smith, R. (1981): Immunization with Purified Protein Antigens from Streptococcus mutans against Dental Caries in Rhesus Monkeys, Infect Immun34:407-415.
13.
Loesche, W.J.; Rowan, J.; Straffon, L.H.; and Loos, P.J. (1975): Association of Streptococcus mutans with Human Dental Decay, Infect Immun11:1252-1260.
14.
Loesche, W.J. and Straffon, L.H. (1979): Longitudinal Investigation of the Role of Streptococcus mutans in Human Fissure Decay, Infect Immun26:498-507.
15.
Ma, J.K.C.; Smith, R.; and Lehner, T. (1987): Use of Monoclonal Antibodies in Local Passive Immunization to Prevent Colonization of Human Teeth by Streptococcus mutans , Infect Immun55:1274-1278.
16.
Markwell, M.A.K.; Haas, S.M.; Bieber, L.L.; and Tol-Bert, N.E. (1978): A Modification of the Lowry Procedure to Simplify Protein Determination in Membrane and Lipoprotein Samples, Anal Biochem87:206-210.
17.
McGhee, J.R.; Michalek, S.M.; Navia, J.M.; and Nar-Kates, A.J. (1976): Effective Immunity to Dental Caries: Study of Active and Passive Immunity to Streptococcus mutans in Malnourished Rats, J Dent Res55:C206-C214.
18.
Michalek, S.M.; Gregory, R.L.; Harmon, C.C.; Katz, J.; Richardson, G.J.; Hilton, T.; Filler, S.J.; and McGhee, J.R. (1987): Protection of Gnotobiotic Rats against Dental Caries by Passive Immunization with Bovine Milk Antibodies to Streptococcus mutans, Infect Immun55:2341-2347.
19.
Michalek, S.M. and McGhee, J.R. (1977): Virulence of Streptococcus mutans: An Antibiotic-suppressed Rat Model for Studies of Pathogenesis, J Dent Res56:205-211.
20.
Michalek, S.M.; McGhee, J.R.; Arnold, R.R.; and Mes-Tecky, J. (1978): Effective Immunity to Dental Caries: Selective Induction of Secretory Immunity by Oral Administration of Streptococcus mutans in Rodents, Adv Exp Med Biol107 :261-269.
21.
Michalek, S.M.; Morisaki, I.; Gregory, R.L.; Kiyono, H.; Hamada, S.; and McGhee, J.R. (1983): Oral Adjuvants Enhance IgA Responses to Streptococcus mutans, Mol Immunol20:1009-1018.
22.
Patterson, R.; Youngner, J.S.; Weigle, W.O.; and Dixon, F.J. (1962): Antibody Production and Transfer to Egg Yolk in Chickens, J Immunol89:272-278.
23.
Shimizu, M.; Fitzsimmons, R.C.; and Nakai, S. (1988): Anti-E. coli Immunoglobulin Y Isolated from Egg Yolk of Immunized Chickens as a Potential Food Ingredient, J Food Sci53 :1360-1366.
24.
Smith, D.J.; Taubman, M.A.; and Ebersole, J.L. (1978): Effects of Local Immunization with Glucosyltransferase Fractions from Streptococcus mutans on Dental Caries in Hamsters Caused by Homologous and Heterologous Serotypes of Streptococcus mutans, Infect Immun21:843-851.
25.
Smith, D.J.; Taubman, M.A.; and Ebersole, J.L. (1979): Effect of Oral Administration of Glucosyltransferase Antigens on Experimental Dental Caries, Infect Immun26:82-89.
26.
Stinson, M.W. and Bergey, E.J. (1982): Isolation of a Heart-and Kidney-binding Protein from Group A Streptococci, Infect Immun35:335-342.
27.
Van de Run, I.; Bleiweis, A.S.; and Zabriskie, J.B. (1976): Antigens in Streptococcus mutans Cross-reactive with Human Heart Muscle, J Dent Res55:C59-C64.
