Neutrophil myeloperoxidase (MPO) adsorbs to bacteria as a pre-requisite for killing by the MPO/hydrogen-peroxide/chloride system. Three chromatographically distinct isoforms of MPO (MPO I, MPO II, and MPO III) have been isolated from human neutrophils. The purpose of this study was to determine whether oral bacteria-including Actinobacillus actinomycetemcomitans, Capnocytophaga sputigena, Haemophilus aphrophilus, and Eikenella corrodens-differentially adsorb the three major isoforms of MPO from a mixture of MPO I, II, and III, and to assess the effect of pH and normal human serum (NHS) on MPO adsorption. MPO III adsorbed preferentially (i) at high bacterial concentrations, (ii) in the pH range of 6.0-8.0, and (iii) in the presence and absence of NHS. These results support the role of MPO III in the killing of oral bacteria.
Get full access to this article
View all access options for this article.
References
1.
Baker, P.J. and Wilson, M.E. (1988): Effect of Clindamycin on Neutrophil Killing of Gram-negative Periodontal Bacteria, Antimicrob Agents Chemother32:1521-1527.
2.
Chance, B. and Maehly, A.C. (1955): Catalases and Peroxidases, Methods Enzymol2:764-775.
3.
Clark, R.A. (1983): Extracellular Effects of the Myeloperoxidase - Hydrogen Peroxide - Halide System. In: Advances in Inflammation Research, Vol. 5, G. Weissman, Ed., New York: Raven Press, pp. 107-146.
4.
Clark, R.A. and Szot, S. (1982): Chemotactic Factor Inactivation by Stimulated Human Neutrophils Mediated by Myeloperoxidase-catalyzed Methionine Oxidation , J Immunol128:1507-1513.
5.
Diamond, R.D.; Clark, R.A.; and Haudenschild, C.C. (1980): Damage of Candida albicans Hyphae and Pseudohyphae by the Myeloperoxidase System and Oxidative Products of Neutrophil Metabolism in vitro, J Clin Invest66 :908-917.
6.
Hashinaka, K.; Nishio, C.; Hur, S.-J.; Sakiyama, F.; Tsunasawa, S.; and Yamada, M. (1988): Multiple Species of Myeloperoxidase Messenger RNAs Produced by Alternative Splicing and Differential Polyadenylation, Biochemistry27:5906-5914.
7.
Hasilik, A.; Pohlman, R.; Olsen, R.L.; and Von Figura, K. (1984): Myeloperoxidase is Synthesized as a Larger Phosphorylated Precursor, EMBO J3:2671-2676.
8.
Klebanoff, S.J. (1970): Myeloperoxidase: Contribution to the Microbicidal Activity of Intact Leukocytes, Science169:1095-1097.
9.
Matheson, N.R.; Wong, P.S.; and Travis, J. (1981): Interaction of Human α-1-Proteinase Inhibitor with Neutrophil Myeloperoxidase, Biochemistry20 :331-336.
10.
Miyasaki, K.T.; DeCamargo, P.M.; and Wolinsky, L.E. (1988): Differential Modulation of Adherence of Oral Streptococci by Human Neutrophil Myeloperoxidase, J Dent Res67:1300-1306.
11.
Miyasaki, K.T.; Wilson, M.E.; Brunetti, A.J.; and Genco, R.J. (1986a): Oxidative and Nonoxidative Killing of Actinobacillus actinomycetemcomitans by Human Neutrophils, Infect Immun53:154-160.
12.
Miyasaki, K.T.; Wilson, M.E.; and Genco, R.J. (1986b): Killing of Actinobacillus actinomycetemcomitans by the Human Neutrophil Myeloperoxidase-Hydrogen Peroxide-Chloride System , Infect Immun53:161-165.
13.
Miyasaki, K.T.; Wilson, M.E.; Cohen, E.; Jones, P.C.; and Genco, R.J. (1986c): Evidence for and Partial Characterization of Three Major and Three Minor Chromatographic Forms of Human Neutrophil Myeloperoxidase , Arch Biochem Biophys246:751-764.
14.
Miyasaki, K.T.; Zambon, J.J.; Jones, C.A.; and Wilson, M.E. (1987): Role of High Avidity Binding of Human Neutrophil Myeloperoxidase in the Killing of Actinobacillus actinomycetemcomitans, Infect Immun55:1029-1036.
15.
Ooi, W.; Levine, H.G.; Lamont, J.T.; and Clark, R.A. (1984): Inactivation of Clostridium difficile Cytotoxin by the Neutrophil Myeloperoxidase System, J Infect Dis149:215-219.
16.
Pember, S.O. and Kinkade, J.M., Jr. (1983): Differences in Myeloperoxidase Activity from Neutrophilic Polymorphonuclear Leukocytes of Differing Density: Relationship to Selective Exocytosis of Distinct Forms of the Enzyme, Blood61:1116-1124.
17.
Pember, S.O.; Shapira, R.; and Kinkade, J.M., Jr. (1983): Multiple Forms of Myeloperoxidase from Human Neutrophilic Granulocytes: Evidence for Differences in Compartmentalization , Enzymatic Activity, and Subunit Structure, Arch Biochem Biophys221:391-403.
18.
Selvaraj, R.J.; Zgliczynski, J.M.; Paul, B.B.; and Sbarra, A.J. (1978): Enhanced Killing of Myeloperoxidase-coated Bacteria in the Myeloperoxidase-H2O2-Cl- System, J Infect Dis137:481-485.
19.
Suzuki, K.; Yamada, M.; Akashi, K.; and Fujikura, T. (1986): Similarity of Kinetics of Three Types of Myeloperoxidase from Human Leukocytes and Four Types from HL-60 Cells, Arch Biochem Biophys245:167-173.
20.
Tsai, C.-C.; Hammond, B.F.; Baehni, P.; McArthur, W.P.; and Taich-Man, N.S. (1978): Interaction of Inflammatory Cells and Oral Microorganisms. VI. Exocytosis of PMN Lysosomes in Response to Gram-negative Plaque Bacteria , J Periodont Res13: 504-512.
21.
Van Dyke, T.E.; Wilson-Burrows, C.; Offenbacher, S.; and Henson, P. (1987): Association of an Abnormality of Neutrophil Chemotaxis in Human Periodontal Disease with a Cell Surface Protein, Infect Immun55 :2262-2267.
22.
Weiss, S.J.; Peppin, G.; Ortiz, X.; Ragsdale, C.; and Test, S.T. (1985): Oxidative Autoactivation of Latent Collagenase by Human Neutrophils, Science227:747-749.
23.
Wright, C.D.; Bowie, J.U.; Gray, G.R.; and Nelson, R.D. (1983): Candidacidal Activity of Myeloperoxidase: Mechanisms of Inhibitory Influence of Soluble Cell Wall Mannan, Infect Immun42:76-80.
24.
Wright, J.; Bastian, N.; Davis, T.A.; Zuo, C.; Yoshimoto, S.; Orme-Johnson, W.H.; and Tauber, A.I. (1990): Structural Characterization of the Isoenzymatic Forms of Human Myeloperoxidase: Evaluation of the Iron-containing Prosthetic Group, Blood75:238-241.
25.
Wright, J.; Yoshimoto, S.; Offner, G.D.; Blanchard, R.A.; Trox-Ler, R.; and Tauber, A.I. (1987): Structural Characterization of the Isoenzymatic Forms of Human Myeloperoxidase, Biochim Biophys Acta915:68-76.
26.
Yamada, M.; Mori, M.; and Sugimura, T. (1981): Purification and Characterization of Small Molecular Weight Myeloperoxidase from Human Promyelocytic Leukemia HL-60 Cells, Biochemistry20:766-771.
27.
Zgliczynski, J.M. (1980): Characteristics of Myeloperoxidase from Neutrophils and Other Peroxidases from Different Cell Types. In: The Reticuloendothelial System. A Comprehensive Treatise. 2. Biochemistry and Metabolism, A.J. Sbarra and R.R. Strauss, Eds., New York: Plenum Press, pp. 255-278.
