Changes in Protein Secretion by Rat Submandibular Glands in Response to Isoproterenol,a-Methylnoradrenaline,and Clonidine during Post-natal Development
Restricted accessResearch articleFirst published online January, 1990
Changes in Protein Secretion by Rat Submandibular Glands in Response to Isoproterenol,a-Methylnoradrenaline,and Clonidine during Post-natal Development
We studied developmental changes in salivary volumes and proteins secreted by the submandibular glands of male rats at weekly intervals from two to ten weeks of age in response to the β1-, α1-, and α2- adrenoceptor agonists, isoproterenol (IPR), a-methylnoradrenaline (a-mNA), and clonidine (Clonid). The types of proteins in saliva samples were determined and compared by isoelectric-focusing electrophoresis with the Phast system in both the gradient pH -3.5-to-5 and pH-3.5-to-9 gels by means of silver staining. Salivary volume and protein concentration in saliva samples elicited by IPR and a-mNA were positively related to the weight of the submandibular glands up to six or seven weeks of age, whereas in saliva elicited by Clonid, no relation was found. The isoelectric-focusing electrophoretic patterns of proteins secreted by the glands in response to three stimuli were different from each other during post-natal development. Within one stimulation, differences were also observed at two and three weeks of age for Clonid, and from seven weeks of age for the three stimuli, respectively. The a-type proteins, but not the β-type proteins, were very similar to those in extracts from glands of rats at seven weeks of age. Almost all of the a-type proteins, but not the β-type proteins, reacted with antibodies to two proteases. We conclude that functional maturation precedes morphological maturation in the submandibular glands of rats.
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References
1.
Abe, K. (1987): Autonomic Effects on Protein Secretion by Rat Submandibular Salivary Glands, Comp Biochem Physiol88C :241-248.
2.
Abe, K. and Dawes, C. (1978): The Effects of Electrical and Pharmacological Stimulation on the Types of Proteins Secreted by Rat Parotid and Submandibular Glands, Arch Oral Biol23:367-372.
3.
Abe, K. and Dawes, C. (1980): The Secretion of Protein and of Some Electrolytes in Response to a- and (3-adrenergic Agonists by Rat Parotid and Submandibular Salivary Glands Enlarged by Chronic Treatment with Isoproterenol, J Dent Res59:1081-1089.
4.
Abe, K. and Dawes, C. (1982): Dopamine-induced Secretion of Protein and of Some Electrolytes by Rat Submandibular and Parotid Glands, Arch Oral Biol27:635-643.
5.
Abe, K. and Dawes, C. (1984): The Effects of α-methylnoradrenaline on Protein and Electrolyte Secretion by Rat Submandibular and Parotid Glands, Comp Biochem Physiol78C:383-389.
6.
Abe, K. and Dawes, C. (1985): The Effects of Oxymetazoline on Secretion of Protein and Some Electrolytes by Rat Submandibular and Parotid Glands, Comp Biochem Physiol80C:347-353.
7.
Abe, K.; Hidaka, S.; Ishibashi, K.; Yanabu, M.; Kamogashira, K.; Itoh, T.; and Matsumoto, M. (1987): Developmental Changes in the Volumes, Protein, and Some Electrolyte Concentrations of Male and Female Rat Submandibular Saliva Secreted in Response to Methoxamine and Pilocarphie, J Dent Res66:745-750.
8.
Abe, K.; Yoneda, K.; Fujita, R.; Yokota, Y.; and Dawes, C. (1980): The Effects of Epinephrine, Norepinephrine, and Phenylephrine on the Types of Proteins Secreted by Rat Salivary Glands, J Dent Res59:1627-1634.
9.
Abe, S. (1985): Sex Differences of the α1-Adrenoceptor Functions with Aging in Rat Submandibular Glands, Jpn J Oral Biol27 :909-932 (in Japanese).
10.
Barka, T. and Van der Noen, H. (1974): Adenylate Cyclase Activity in Rat Submandibular Gland During Postnatal Development, Life Sci14:267-280.
11.
Baum, B.J.; Kuyatt, B.L.; and Humphreys, S. (1983): Protein Production and Processing in Young Adult and Aged Rat Submandibular Gland Cells in vitro, Mech Aging Dev23 :123-136.
12.
Bunge, R.; Johnson, M.; and Ross, C.D. (1978): Nature and Nurture in Development of the Autonomic Neurons, Science199:1409-1416.
13.
Bylund, D.B.; Martinez, J.R.; Camden, J.; and Jones, S.B. (1982): Autonomic Receptors in the Developing Submandibular Glands of Neonatal Rats, Arch Oral Biol27:945-950.
14.
Champlain, J.; DE Malmfors, T.; Olson, L.; and Sachs, C. (1970): Ontogenesis of Peripheral Adrenergic Neurons in the Rat: Pre- and Postnatal Observations, Acta Physiol Scand80 :276-288.
15.
Cutler, L.S. and Chaudhry, A.P. (1974): Cytodifferentiation of the Acinar Cells of the Rat Submandibular Gland, Dev Biol41:31-41.
16.
Cutler, L.S. and Chaudhry, A.P. (1975): Cytodifferentiation of Striated Duct Cells and Secretory Cells of the Convoluted Granular Tubules of the Rat Submandibular Gland, Am J Anat143 :201-217.
17.
Cutler, L.S.; Christian, C.P.; and Bottaro, B. (1981): Development of Stimulus-Secretion Coupling in Salivary Glands, Mech Cell Biol23:531-545.
18.
Denny, P.C. and Cope, P. (1977): Age-specific Differentiations During the Development of the Rat Submandibular Gland, Differentiation8:105-111.
19.
Fukuda, M. (1967): Histochemical Studies on the Rat Submaxillary Gland During Post-natal Development, Histochemistry8:342-354.
20.
Grand, R.J.; Chong, D.A.; and Ryan, S.J. (1975): Postnatal Development of Adenylate Cyclase in Rat Salivary Glands: Patterns of Hormonal Sensitivity, Am J Physiol228:608-612.
21.
Gresik, E.W. (1980): Postnatal Developmental Changes in Submandibular Glands of Rats and Mice, J Histochem Cytochem28:860-870.
22.
Heukeshoven, J. and Dernick, R. (1985): Simplified Method for Silver Staining of Proteins in Polyacrylamide Gels and the Mechanism of Silver Staining, Electrophoresis6:103-112.
23.
Ikeno, K.; Kuzuya, H.; and Ishiguro, I. (1984): Developmental Changes of Trypsin-like Protease Activity in the Submandibular Glands of Male and Female Young Growing Rats , Arch Oral Biol29:669-674.
24.
Inanaga, T.; Habu, T.; Tanaka, E.; Taniguchi, T.; Nish-Iura, T.; Ishibashi, K.; Naruse, S.; and Abe, K. (1988): Age Changes in Secretory Function of Male and Female Rat Parotid Glands in Response to Methoxamine and Pilocarpine, J Dent Res67:565-573.
25.
Ito, H.; Baum, B.J.; and Roth, G.S. (1981): β-adrenergic Regulation of Rat Parotid Gland Exocrine Protein Secretion During Aging, Mech Aging Dev15:177-188.
26.
Jacoby, F. and Leeson, C.R. (1959): The Postnatal Development of the Rat Submaxillary Gland, J Anat93:201-216.
27.
Kamogashira, K.; Yanabu, M.; Ichikawa, K.; Itoh, T.; Matsumoto, M.; Ishibashi, K.; and Abe, K. (1988): The Effects of Upper Incisor Separation on the Submandibular and Sublingual Glands of Rats, J Dent Res67:602-610.
28.
Kim, S.K.; Han, S.S.; and Nasjleti, C.E. (1970): The Fine Structure of Secretory Granules in Submandibular Glands of the Rat During Early Postnatal Development, Anat Rec168:463-476.
29.
Laemmli, U.K. (1970): Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4, Nature227:680-685.
30.
Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; and Ran-Dall, R.J. (1951): Protein Measurement with the Folin Phenol Reagent , J Biol Chem193:265-275.
31.
Martinez, J.R. and Camden, J. (1983): Volume and Composition of Pilocarpine- and Isoproterenol-stimulated Submandibular Saliva of Early Postnatal Rats, J Dent Res62:543-547.
32.
Matthews, R.W. (1974): The Effects of Autonomic Stimulation Upon the Rat Submandibular Gland, Arch Oral Biol19:989-994.
33.
Menaker, L. and Miller, S.A. (1973): Changes in Nucleic Acid and Protein Content During Development of the Rat Submandibular Gland, Arch Oral Biol18:1325-1336.
34.
Muenzer, J.; Bildstein, C.; Gleason, M.; and Carlson, D.M. (1979a): Purification of Proline-rich Proteins from Parotid Glands of Isoproterenol-treated Rats, J Biol Chem254:5623-5628.
35.
Muenzer, J.; Bildstein, C.; Gleason, M.; and Carlson, D.M. (1979b): Properties of Proline-rich Proteins from Parotid Glands of Isoproterenol-treated Rats, J Biol Chem254:5629-5634.
36.
Ørstavik, T.B.; Nustad, K.; and Brandtzaeg, P. (1977): A Biochemical and Immunohistochemical Study of Kallikrein in Normal and Isoproterenol-stimulated Rat Salivary Glands During Postnatal Development, Arch Oral Biol22:495-502.
37.
Sreebny, L.M.; Meyer, J.; Bachem, E.; and Weinmann, J.P. (1955): Postnatal Changes in Proteolytic Activity and in the Morphology of the Submaxillary Gland in Male and Female Albino Rats , Growth19:57-74.
38.
Srinivasan, R. and Chang, W.W.L. (1975): The Development of the Granular Convoluted Duct in the Rat Submandibular Gland, Anat Rec182:29-40.
39.
Towbin, H.; Staehelin, T.; and Gordon, J. (1979): Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications, Proc Natl Acad Sci USA76:4350-4354.
40.
Yamashina, S. and Barka, T. (1974): Peroxidase Activity in the Developing Rat Submandibular Gland, Lab Invest31:82-89.
41.
Yoshida, Y.; Sprecher, R.L.; Schneyer, C.A.; and Schneyer, L.H. (1967): Role of β-Receptors in Sympathetic Regulation of Electrolytes in Rat Submaxillary Saliva, Proc Soc Exp Biol Med126:912-916.
42.
Yu, J.H.; Mark, M.R.; and Redman, R.S. (1987): Effect of Clonidine on Secretion of Fluid and Ions by the Parotid and Submandibular Glands of the Rat, Arch Oral Biol32:27-33.
