Microscopic and Crystallographic Examinations of the Teeth of the X-linked Hypophosphatemic Mouse

Abstract

Teeth from the C57BLl6J-Hyp mouse (Hyp mouse) were examined histologically, radiographically, and crystallographically. Microscopic examinations of the ground sections, the decalcified and H-E-stained sections, and the contact microradiograms of molars and incisors from the Hyp mouse showed several abnormalities-such as large pulp chamber, wide predentin, thin dentin at the pulp floor, and multiple occurrences of interglobular dentin. Powder and microbeam x-ray diffraction analyses showed that the crystallinity of hydroxyapatite in incisor globular dentin from the Hyp mouse was higher than that in incisor dentin from the normal mouse. On the other hand, the findings of transmission electron microscopy demonstrated that the sizes of hydroxyapatite crystals of globular dentin in the Hyp incisor were larger than those in normal incisor dentin. These results demonstrated that the findings in Hyp mouse teeth were in accord with those of human XLH teeth.

Get full access to this article

View all access options for this article.

References

Abe, K. ; Ooshima, T. ; Tong, S.M.L. ; Yasufuku, Y. ; and Sobue, S. (1988): Structural Deformities of Deciduous Teeth in Patients with Hypophosphatemic Vitamin D-Resistant Rickets, Oral Surg Oral Med Oral Pathol 65:191-198.

Abe, K. ; Ooshima, T. ; Sobue, S. ; and Moriwaki, Y. (1989): The Crystallinity of Deciduous Teeth in Hypophosphataemic Vitamin D-Resistant Rickets, Arch Oral Biol 34:365-372.

Archard, H.O. and Witkop, C.J. (1966): Hereditary Hypophosphatemia (Vitamin D-Resistant Rickets) Presenting Primary Dental Manifestations, Oral Surg Oral Med Oral Pathol 22:184-193.

Eicher, E.M. ; Southard, J.L. ; Scriver, C.R. ; and Glorieux, F.H. (1976): Hypophosphatemia: Mouse Model for Human Familial Hypophosphatemic (Vitamin D-Resistant) Rickets, Proc Natl Acad Sci USA 73:4667-4671.

Iorio, R.J. ; Bell, W.A. ; Meyer, M.H. ; and Meyer, R.A., Jr. (1979): Histologic Evidence of Calcification Abnormalities in Teeth and Alveolar Bone of Mice with X-Linked Dominant Hypophosphatemia (VDRR), Ann Dent 38:38-44.

Klug, H.P. and Alexander, L.E. (1966): Crystallite-Size Determination from Line Broadening . In: X-ray Diffraction Procedures, New York: John Wiley & Sons, Chap. 9, pp. 491-538.

Meyer, R.A., Jr. ; Jowsey, J. ; and Meyer, M.H. (1979): Osteomalacia and Altered Magnesium Metabolism in the X-Linked Hypophosphatemic Mouse, Calcif Tissue Int 27:19-26.

Nikiforuk, G. and Fraser, D. (1979): Etiology of Enamel Hypoplasia and Interglobular Dentin: The Roles of Hypocalcemia and Hypophosphatemia, Metab Bone Dis Rel Res 2:17-23.

Rasmussen, H. and Anast, C. (1983): Familial Hypophosphatemic (Vitamin D-Resistant) Rickets and Vitamin D-Dependent Rickets. In: The Metabolic Basis of Inherited Disease, J.B. Stanbury , J.B. Wyngaarden , and D.S. Fredrickson , Eds., New York: McGraw-Hill, pp. 1743-1773.

10.

Yasufuku, Y. ; Kohno, N. ; Tsutsumi, N. ; Ooshima, T. ; Sobue, S. ; Murakami, Y. ; and Ikari, H. (1983): Dental Management of Familial Hypophosphatemic Vitamin D-Resistant Rickets: Report of Case, J Dent Child 50:300-304.