The purpose of this study was to examine the change in plaque area over nine days in vivo on four materials with different surface free-energies (s.f.e.). Twelve healthy dental students participated in a crossover, split-mouth, double-blind study. Supragingival plaque formation was recorded over a nine-day period, on four different materials: fluorethylenepropylene (Teflon) (FEP), parafilm (PAR), cellulose acetate (CA), and enamel (E) with s.fe. of 20, 26, 57, and 88 erg/cm2, respectively. Strips made from the first three materials were stuck to the buccal surface of an upper incisor. The remaining incisor was carefully polished and served as an enamel surface. The increase in plaque was evaluated after three, six, and nine days. A planimetrical analysis was used so that the plaque area could be expressed as a percentage of the total buccal tooth surface. This procedure was repeated on each subject, so that at the end, each pair of central or lateral incisors received the four tested materials.
The results indicated that the adherence of micro-organisms on pellicle-coated substrata was influenced by the material's s.f.e.; there was an association between the s.f.e. of the substrata and the supragingival plaque extension in vivo. High surface free-energy substrata in the oral cavity attracted more micro-organisms than did low energetic materials. Additionally, the bacterial adhesion seemed very weak on surfaces with a low s.f.e.
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References
1.
Absolom, D.R.; Lamberti, E.V.; Policova, Z.; Zingg, W.; Van Oss, C.J.; and Neumann, A.W. (1983): Surface Thermodynamics of Bacterial Adhesion, Appl Env Microbiol46:90-97.
2.
Baier, R.E. (1984): Adhesion in the Biologic Environment, Biomater Med Dev Artif Organs12:133-159.
3.
Busscher, H.J.; Kip, G.A.M.; Van Silfhout, A.; and Arends, J. (1986b): Spreading Pressures of Water and n-Propanol on Polymer Surfaces, J Colloid Interf Sci114:307-313.
4.
Busscher, H.J.; Uyen, H.M.; Weerkamp, A.H.; Postma, W.J.; and Arends, J. (1986a): Reversibility of Adhesion of Oral Streptococci to Solids, FEMS Microbiol Lett35:303-306.
5.
Busscher, H.J.; Van Pelt, A.W.J.; De Jong, H.P.; and Arends, J. (1983): Effect of Spreading Pressure on Surface Energy Determination by Means of Contact Angles, J Colloid Interf Sci95:23-27.
6.
Busscher, H.J.; Weerkamp, A.H.; Van Der Mei, H.C.; Van Pelt, A.W.J.; DE Jong, H.P.; and Arends, J. (1984): Measurements of the Surface Free Energy of Bacterial Cell Surfaces and its Relevance for Adhesion, Appl Env Microbiol48:980-983.
7.
Busscher, H.J. and Weerkamp, A.H. (1987): Specific and Non-specific Interactions in Bacterial Adhesion to Solid Substrata, FEMS Microbiol Reviews46:165-173.
8.
Cimasoni, G. (1974): The Crevicular Fluid, Basel : Karger, pp. 1-122.
9.
Crisp, D.J.; Walker, G.; Young, G.A.; and Yule, A.B. (1985): Adhesion and Substrate Choice in Mussels and Barnacles , J Colloid Interf Sci104:40-50.
10.
De Jong, H.P.; De Boer, P.; Busscher, H.J.; Van Pelt, A.W.J.; and Arends, J. (1984): Surface Free Energy Changes of Human Enamel during Pellicle Formation - An in vivo Study, Caries Res18:408-415.
11.
De Jong, H.P.; Van Pelt, A.W.J.; and Arends, J. (1982): Contact Angle Measurements on Human Enamel - An in vitro Study of Influence of Pelliclc and Storage Period, J Dent Res6:11-13.
12.
Dexter, S.C. (1979): Influence of Substratum Critical Surface Tension on Bacterial Adhesion - in situ Studies, J Colloid Interf Sci70:346-354.
13.
Dexter, S.C. and Lucas, K.E. (1985): The Study of Biofilm Formation Under Water by Photoacoustic Spectroscopy, J Colloid Interf Sci104:15-27.
14.
Doyle, R.J.; Nesbitt, W.E.; and Taylor, K.G. (1982): On the Mechanism of Adherence of Streptococcus sanguis to Hydroxyapatite, FEMS Microbiol Lett15:1-5.
15.
Fletcher, R.L. and Baier, R.E. (1984): Influence of Surface Energy on the Development of the Green Alga Entcromorpha, Mar Biol Lett5:251-254.
16.
Glantz, P.O. (1969): On Wettability and Adhesiveness, Odont Revy20 (Suppl 17):5-124.
17.
Grinnell, F.; Milam, M.; and Srere, P.A. (1973): Attachment of Normal and Transformed Hamster Kidney Cells to Substrata Varying in Chemical Composition, Biochem Med J: 87-90.
18.
Hlady, V.; Reinecke, D.R.; and Andrade, J.D. (1986) : Fluorescence of Adsorbed Protein Layers 1. Quantitation of Total Internal Reflection Fluorescence, J Colloid Interf Sci111:555-569.
19.
Milliken, G.A. and Johnson, D.E. (1984): Analysis of Messy Data, Belmont : Lifetime Learning Publications, p. 156.
20.
Morganstein, S.I. and Ribbons, J.W. (1969): The Inhibitory Effect of Disclosing Solutions on Plaque Growth and Culture, J Dent Res48:1100 (Abst. 12).
21.
Mühlemann, H.R. and Son, S. (1971): Gingival Sulcus Bleeding - A Leading Symptom in Initial Gingivitis, Helv Odont Acta15:107-113.
22.
Newman, M.G. (1985): Current Concepts of the Pathogcnesis of Periodontal Disease, J Periodontol56:734-739.
23.
Olsson, J.; Glantz, P.O.; and Krasse, B. (1976): Surface Potential and Adherence of Oral Streptococci to Solid Surfaces, Scand J Dent Res84 :240-242.
24.
Pratt-Terpstra, I.H.; Weerkamp, A.H.; and Busscher, H.J. (1987): Adhesion of Oral Streptococci from a Flowing Suspension to Uncoated and Albumin-coated Surfaces, J Gen Microbiol133:3199-3206.
25.
Quirynen, M. (1986): Anatomical and Inflammatory Factors Influence Bacterial Plaque Growth and Retention in Man. Thesis, Catholic University Leuven, Belgium.
26.
Quirynen, M.; Marechal, M.; Busscher, H.J.; Weerkamp, A.H.; Darius, P.L.; and van Steenberghe, D. (1989): The Role of the Surface Free Energy and the Surface Roughness in the Early in vivo Formation of Bacterial Plaque on Polymeric Substrata, J Clin Periodontol (submitted).
27.
Quirynen, M.; van Steenberghe, D.; and Vuylsteke, M. (1985): The Possibility of Measuring Plaque Growth in vivo Within 24 Hours, J Periodont Res20:321-328.
28.
Rosenberg, M. and Kjelleberg, S. (1986): Hydrophobic Interactions: Role in Bacterial Adhesions , Microbiol Ecol9:353-393.
29.
Schakenraad, J.M.; Busscher, H.J.; Wildevuur, C.R.H.; and Arends, J. (1986): The Influence of Substratum Free Energy on Growth and Spreading of Human Fibroblasts in the Presence and Absence of Serum Proteins , J Biomat Res20:773-784.
30.
Siochi, E.J.; Eiss, N.S.; Gilliam, D.R.; and Wightman, J.P. (1987): A Fundamental Study of the Sticking of Insect Residues to Aircraft Wings, J Colloid Interf Sci115:346-356.
31.
Syed, S.A. and Loesche, W.J. (1978): Bacteriology of Human Experimental Gingivitis - Effect of Plaque Age, Infect Immun21:821-829.
32.
Uyen, H.M.; Busscher, H.J.; Weerkamp, A.H.; and Arends, J. (1985): Surface Free Energies of Oral Streptococci and their Adhesion to Solids, FEMS Microbiol Leu30:103-106.
33.
Van Dijk, L.J.; Goldsweer, R.; and Busscher, H.J. (1988): Interfacial Free Energy as a Driving Force for Pellicle Formation in the Oral Cavity: An in vivo Study in Beagle Dogs, Biofouling1:19-25.
34.
Van Dijk, L.J.; Herkstroter, F.; Busscher, H.; Weerkamp, A.; Jansen, H.; and Arends, J. (1987): Surface-free Energy and Bacterial Adhesion - An in vivo Study in Beagle Dogs, J Clin Periodontol14 :300-304.
35.
Van Pelt, A.W.J.; De Jong, H.P.; Busscher, H.J.; and Arends, J. (1983): Dispersion and Polar Surface Free Energies of Human Enamel - An in vitro Study, J Biomed Mat Res17:637-643.
36.
Van Pelt, A.W.J.; Van Der Mei, H.C.; Busscher, H.J.; Arends, J.; and Weerkamp, A.H. (1984): Surface Free Energies of Oral Strcptococci, FEMS Microbiol Lett25:279-282.
37.
Van Pelt, A.W.J.; Weerkamp, A.H.; Uyen, M.H.W.; Busscher, H.J.; DE Jong, H.P.; and Arends, J. (1985): Adhesion of Streptococcus sanguis CH3 to Polymers with Different Surface Free Energies, Appl Environ Microbiol49:1270-1275.
38.
Weerkamp, A.H.; Quirynen, M.; Marechal, M.; Van Der Mei, H.C.; van Steenberghe, D.; and Busscher, H.J. (1989): The Role of Surface Free Energy in the Early in vivo Formation of Dental Plaque on Human Enamel and Polymeric Substrata, Microbial Ecology in Health and Disease (accepted).
39.
Weerkamp, A.H.; Van Der Mei, H.C.; and Busscher, H.J. (1985): The Surface Free Energy of Oral Streptococci after Coating with Saliva and its Relation to Adhesion in the Mouth, J Dent Res64:1204-1210.
40.
Weerkamp, A.H.; Van Der Mei, H.C.; Engelen, D.P.E.; and De Windt, C.E.A. (1984): Adhesion Receptors (Adhcsins) of Oral Streptococci. In: Proceedings, Bacterial Adhesion and Preventive Dentistry, J.M. ten Catc, S.A. Leach, and J. Arends , Eds., Oxford: IRL Press Ltd , pp. 85-97.
