In this study, the modulation of adherence of hydrogen peroxide (H2O2)-producing and non-H2O2-producing strains of oral streptococci by the host leukocyte enzyme myeloperoxidase (MPO) was examined. It was found that exposure to MPO decreased adherence of many strains of oral streptococci to saliva-coated hydroxyapatite beads in the presence of exogenous H2O 2 and chloride. The MPO-H2O2-Cl- system increased the adherence of one strain. In the absence of exogenous H 2O2, the MPO-H2O2-Cl- system decreased the adherence of H2O2producing strains only. Glucose increased streptococcal H2O2 production and also increased the anti-adhesive activity of MPO in the absence of exogenous H2O2. We conclude that: (1) host leukocytes can modulate the adherence of oral streptococci via MPO; (2) endogenous production of H 2O2 by the oral streptococci can provide sufficient substrate H2O2 to drive this system; and (3) MPO will exert differential modulatory effects on the adherence of oral streptococci, based in part upon the level of endogenous H2O2 production and in part upon the particular characteristics of the adhesins of the bacteria.
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References
1.
Applebaum, B.; Golub, E.; Holt, S.C.; and Rosan, B. (1979): In vitro Studies of Dental Plaque Formation: Adsorption of Oral Streptococci to Hydroxyapatite, Infect Immun25:717-728.
2.
Bradford, M.M. (1976): A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Anal Biochem7:248-254.
3.
Carlsson, J.; Iwami, Y.; and Yamada, T. (1983): Hydrogen Peroxide Excretion by Oral Streptococci and Effect of Lactoperoxidase-Thiocyanate-Hydrogen Peroxide, Infect Immun40:70-80.
4.
Chance, B. and Maehly, A.C. (1955): Catalases and Peroxidases, Methods Enzymol2:764-775.
5.
Charon, J.A.; Mergenhagen, S.E.; and Gallin, J.I. (1985): Gingivitis and Oral Ulceration in Patients with Neutrophil Dysfunction, J Oral Pathol14:150-155.
6.
Cimasoni, G.; Song, M.; and McBride, B.C. (1987): Effect of Crevicular Fluid and Lysosomal Enzymes on the Adherence of Streptococci and Bacteroides to Hydroxyapatite, Infect Immun55:1484-1489.
7.
Clark, R.A. (1983): Extracellular Effects of the Myeloperoxidase-Hydrogen Peroxide-Halide System. In: Advances in Inflammation Research, Vol. 5, G. Weissman, Ed., New York: Raven Press, pp. 107-146.
8.
Clark, R.A.; Leidal, K.G.; and Taichman, N.S. (1986): Oxidative Inactivation of Actinobacillus actinomycetemcomitans Leukotoxin by the Neutrophil Myeloperoxidase System, Infect Immun53:252-256.
9.
Clark, W.B.; Baumann, L.L.; and Gibbons, R.J. (1978): Comparative Estimates of Bacterial Affinities and Adsorption Sites on Hydroxyapatite Surfaces, Infect Immun19:846-853.
10.
Cowan, M.M.; Taylor, K.G.; and Doyle, R.J. (1986): Kinetic Analysis of Streptococcus sanguis Adhesion to Artificial Pellicle, J Dent Res65:1278-1283.
11.
Cowan, M.M.; Taylor, K.G.; and Doyle, R.J. (1987): Role of Sialic Acid in the Kinetics of Streptococcus sanguis Adhesion to Artificial Pellicle, Infect Immun55:1552-1557.
12.
de Camargo, P.M.; Miyasaki, K.T.; and Wolinsky, L.E. (1988): Host Modulation of Adherence of Oral Bacteria: Effect of Human Neutrophil Myeloperoxidase on the Attachment of Actinomyces viscosus to Saliva-coated Hydroxyapatite, J Periodont Res (accepted for publication).
13.
Doyle, R.J.; Nesbitt, W.E.; and Taylor, K.G. (1982): On the Mechanism of Adherence of Streptococcus sanguis to Hydroxylapatite, FEMS Microbiol Lett15:1-5.
14.
Elder, L.B. and Fives-Taylor, P. (1986): Characterization of Monoclonal Antibodies Specific for Adhesion: Isolation of an Adhesin of Streptococcus sanguis FW213, Infect Immun53:421-427.
15.
Gibbons, R.J. and Van Houte, J. (1975): Bacterial Adherence in Oral Microbial Ecology , Ann Rev Microbiol29:19-44.
16.
Gibbons, R.J.; Etherden, I.; and Moreno, E.C. (1983): Association of Neuraminidase-sensitive Receptors and Putative Hydrophobic Interactions with High-affinity Binding Sites for Streptococcus sanguis C5 in Salivary Pellicles, Infect Immun42:1006-1012.
17.
Greenspan, J.S. and Shillitoe, E.J. (1984): Microbial Pathogenicity in Oral Soft Tissue Diseases , J Dent Res63:431-434.
18.
Kilian, M.; Roland, K.; and Mestecky, J. (1981): Interference of Secretory Immunoglobulin A with Sorption of Oral Bacteria to Hydroxyapatite, Infect Immun31:942-951.
19.
Liljemark, W.F. and Bloomquist, C.G. (1981): Isolation of a Protein-containing Cell Surface Component from Streptococcus sanguis which Affects Its Adherence to Saliva-coated Hydroxyapatite, Infect Immun34:428-434.
20.
Loesche, W.J. (1975): Bacterial Succession in Dental Plaque. Role in Dental Disease, Microbiology44:132-136.
21.
Loesche, W.J.; Gusberti, F.; Mettraux, G.; Higgins, T.; and Syed, S. (1983): Relationship Between Oxygen Tension and Subgingival Microflora in Untreated Human Periodontal Pockets, Infect Immun42:659-667.
22.
Miyasaki, K.T.; Wilson, M.E.; Cohen, E.; Jones, P.C.; and Genco, R.J. (1986a): Evidence for and Partial Characterization of Three Major and Three Minor Chromatographic Forms of Human Neutrophil Myeloperoxidase , Arch Biochem Biophys246:751-764.
23.
Miyasaki, K.T.; Wilson, M.E.; and Genco, R.J. (1986b): Killing of Actinobacillus actinomycetemcomitans by the Human Neutrophil Myeloperoxidase-Hydrogen Peroxide-Chloride System , Infect Immun53:161-165.
24.
Moore, L.V.H.; Moore, W.E.C.; Cato, E.P.; Smibert, R.M.; Burmeister, J.A.; Best, A.M.; and Ranney, R.R. (1987): Bacteriology of Human Gingivitis, J Dent Res66:989-995.
25.
Murray, P.A.; Materese, V.; Hoover, C.I.; and Wink-Ler, J.R. (1987): The Identification of Oral Microbial Lectins by Cell Affinity Chromatography, FEMS Microbiol Lett40:123-127.
26.
Newbrun, E. (1983): Microflora. In: Cariology, 2nd ed., Baltimore : Williams and Wilkins, pp. 50-85.
27.
Pick, E. and Keisara, Y. (1980): A Simple Colorimetric Method for the Measurement of Hydrogen Peroxide Produced by Cells in Culture, J Immunol Methods38:161-170.
28.
Rest, R.F. and Spitznagel, J.K. (1978): Myeloperoxidase-Cl-H2O2 Bactericidal System: Effect of Bacterial Membrane Structure and Growth Conditions , Infect Immun19:1110-1112.
29.
Ronstrom, A.; Edwardsson, S.; and Attstrom, R. (1977): Streptococcus sanguis and Streptococcus salivarius in Early Plaque Formation on Plastic Films, J Periodont Res12:331-339.
30.
Saito, I.; Komiyama, K.; Moro, I.; Akachi, K.; Shiomi, N.; Ito, K.; Murai, S.; and Umemura, S. (1987): Ultrastructural and Immunocytochemical Characterization of Polymorphonuclear Leukocytes from Gingival Crevice in Man, J Periodontol58:493-497.
31.
Staat, R.H.; Langley, S.D.; and Doyle, R.J. (1980): Streptococcus mutans Adherence: Presumptive Evidence for Protein-mediated Attachment Followed by Glucan-dependent Cellular Accumulation , Infect Immun27:675-681.
32.
Tellefson, L.M. and Germaine, G.R. (1986): Adherence of Streptococcus sanguis Hydroxyapatite Coated with Lysozyme and Lysozyme-Supplemented with Saliva, Infect Immun51:750-759.
33.
Tenovuo, J.O. (1985): The Peroxidase System in Human Secretions. In: The Lactoperoxidase System, Chemistry and Biological Significance , K.M. Pruitt and J.O. Tenovuo, Eds., New York: Marcel Dekker, Inc., pp. 101-122.
34.
Thomas, E.L. and Fishman, M. (1986): Oxidation of Chloride and Thiocyanate by Isolated Leukocytes, J Biol Chem261 :9694-9702.
35.
Van Dyke, T.E.; Levine, M.J.; and Genco, R.J. (1982): Periodontal Diseases and Neutrophil Abnormalities . In: Host-Parasite Interactions in Periodontal Diseases, R.J. Genco and S.E. Mergenhagen , Eds., Washington, D.C.: American Society for Microbiology, pp. 235-245.
36.
Wilton, J.M.A. (1982): Polymorphonuclear Leukocytes of the Human Gingival Crevice: Clinical and Experimental Studies of Cellular Function in Humans and Animals. In: Host-Parasite Interactions in Periodontal Diseases, R.J. Genco and S.E. Mergenhagen, Eds., Washington D.C .: American Society for Microbiology, pp. 246-260.
37.
Wolinsky, L.E.; Seto, B.; and Cerveny, R. (1985): Effect of Saliva Substitutes Upon the Binding of Selected Oral Bacteria to Hydroxyapatite, Caries Res19:507-511.
38.
Zambon, J.J. (1985): Actinobacillus actinomycetemcomitans in Human Periodontal Disease, J Clin Periodontol12:1-20.
39.
Zgliczynski, J.M. (1980): Characteristics of Myeloperoxidase from Neutrophils and Other Peroxidases from Other Cell Types. In: The Reticuloendothelial System: A Comprehensive Treatise, Vol. 2, Biochemistry and Metabolism, A. J. Sbarra and R. R. Strauss, Eds., New York: Plenum Press, pp. 255-278.
