Cross-sectional Analysis of Serum Antibody to Oral Streptococcal Antigens in Children

Abstract

Antibodies to S. salivarius, S. sanguis, and S. mutans cells and to glucosyltransferases (GTF) prepared from these micro-organisms were measured in the sera of 133 infants and children by enzyme-linked immunosorbent assay (ELISA). IgG antibody activity to each cell type and GTF was present at birth (presumably derived from maternal transfer) and declined significantly thereafter. IgG antibody levels to S. salivarius and S. sanguis were next detected in young children (2 to < 3 yr group). However, an increase in IgG antibody to S. mutans cells was not seen until children were older (4 to < 8 yr group), possibly reflecting the later colonization of this organism. In contrast, IgG antibody to GTF of all three streptococcal species remained at low levels throughout the first four years of life. IgG antibody to S. mutans GTF was then the first to appear (4 to < 8 yr group). Serum IgA antibodies to all GTFs were not detected until after this time. Fifteen sera were used to develop IgG immunoblots with the GTF antigens. Some positive sera (7/12) demonstrated reaction(s) with GTF from each of the three streptococcal species. Individual sera showed IgG antibody bands to GTF from several serotypes of the mutans streptococci. No immunoblot reaction was observed with GTF and sera (3) from the four-to-seven-year and younger age groups. These results indicate the presence of serum antibody to bacteria and bacterial products associated with plaque formation very early in life and during and after the pre-adolescent years. The potential exists for this serum antibody to modulate bacterial colonization or accumulation in the oral cavity.

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References

Carlsson, J. ; Grahnen, H. ; and Jonsson, G. (1975): Lactobacilli and Streptococci in the Mouth of Children, Caries Res 9:333-339.

Carlsson, J. ; Newbrun, E. ; and Krasse, B. (1969): Purification and Properties of Dextransucrase from Streptococcus sanguis, Arch Oral Biol 14:469-478.

Challacombe, S. and Lehner, T. (1976): Serum and Salivary Antibodies to Cariogenic Bacteria in Man, J Dent Res 55 :C139-C148.

Ebersole, J.L. ; Taubman, M.A. ,; Frey, D.E. ; and Smith, D.J. (1980): An ELISA for Measuring Serum Antibodies to Actinobacillus actinomycetemcomitans, J Periodont Res 15:621-632. Ebersole, J.L. ; Taubman, M.A. ; and Smith, D.J. (1985): Gingival Crevicular Fluid Antibody to Oral Microorganisms. II. Distribution and Specificity of Local Antibody Responses, J Periodont Res 20:349-356.

Fairbanks, G. ; Stack, T.L. ; and Wallach, D.F.H. (1971): Electrophoretic Analysis of the Major Polypeptides of the Human Erythrocyte Membrane, Biochemistry 10:2606-2609.

Gahnberg, L. ; Smith, D.J. ; Taubman, M.A. ; and Ebersole, J.L. (1985): Salivary IgA Antibody to Glucosyltransferase of Oral Microbial Origin in Children, Arch Oral Biol 30:551-556.

Huang, S. ; Lee, H.C. ; and Mayer, R.M. (1979): The Purification and Properties of Dextransucrase from Streptococcus sanguis ATCC 10558, Carbohydr Res 74:287-300.

Krasse, B. ; Gahnberg, L. ; and Emilson, C.G. (1986): Effect of Antibodies on Colonization of Gingival Tooth Surfaces by Streptococcus mutans. In: The Borderland between Caries and Periodontal Disease III, T. Lehner and G. Cimasoni , Eds., Geneva : Editions Médécine et Hygiene.

Laemmli, U.K. (1970): Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4, Nature 227:680-682.

10.

Lehner, T. ; Caldwell, J. ; and Smith, R. (1985): Local Passive Immunization by Monoclonal Antibodies against Streptococcal Antigen I/II in the Prevention of Dental Caries, Infect Immun 50:796-799.

11.

Lehner, T. ; Challacombe, S.J. ; and Caldwell, J. (1975): Immunologic Basis for Vaccination against Dental Caries in Rhesus Monkeys, Nature 254:517-520.

12.

Lehner, T. ; Murray, J.J. ; Winter, G.G. ; and Caldwell, J. (1978): Antibodies to Streptococcus mutans and Immunoglobulin Levels in Children with Dental Caries, Arch Oral Biol 23 :1061-1067.

13.

Lehner, T. ; Russell, M.W. ; and Caldwell, J. (1980): Immunization with a Purified Protein from Streptococcus mutans against Dental Caries in Rhesus Monkey, Lancet 8176:996-997.

14.

Ma, J.K.-C. ; Smith, R. ; and Lehner, T. (1987): Use of Monoclonal Antibodies in Local Passive Immunization to Prevent Colonization of Human Teeth by Streptococcus mutans , Infect Immun 55:1274-1278.

15.

Mancini, G. ; Carbonera, A.O. ; and Heremans, J.P. (1965): Immunochemical Quantitation of Antigens by Single Radial Immunodiffusion, Immunochemistry 2:335-354.

16.

McCabe, M.M. (1985): Purification and Characterization of a Primer-independent Glucosyltransferase from Streptococcus mutans 6715-13 Mutant 27, Infect Immun 50:771-777.

17.

McCabe, M.M. ; Koga, T. ; Inoue, M. ; Freedman, M.L. ; and Hamelik, R.M. (1983): Glucosyltransferase Isozymes from Streptococcus mutans. In: Glucosyltransferases, Sucrose, Glucans and Dental Caries, R.J. Doyle and J. Ciardi , Eds. (a special supplement to Chemical Senses), Washington, D.C.: Information Retrieval, Inc., pp. 70-82.

18.

Michalek, S.M. ; Morisaki, I. ; Gregory, R.L. ; Kiyono, H. ; Hamada, S. ; and McGhee, J.R. (1983): Oral Adjuvants Enhance IgA Responses to Streptococcus mutans, Molec Immunol 20:1009-1018.

19.

Pucci, M.J. ; Tew, J.G. ; and Macrina, F. (1986): Human Serum Antibody Response Against Streptococcus mutans Antigens, Infect Immun 51:600-606.

20.

Russell, R.R.B. (1979): Glycosyltransferases of Streptococcus mutans Strain Ingbritt, Microbios 23:135-146.

21.

Russell, R.R.B. ; Abdulla, E. ; Gilpin, M.L. ; and Smith, K. (1986); Characteristics of Streptococcus mutans Surface Antigens. In: Molecular Microbiology and Immunobiology of Streptococcus mutans, S. Hamada , S.M. Michalek , H. Kiyono , L. Menaker , and J.R. McGhee , Eds., Amsterdam: Elsevier Science Publishers, pp. 61-70.

22.

Smith, D.J. ; Gahnberg, L. ; Taubman, M.A. ; and Ebersole, J.L. (1986): Salivary Antibody Responses to Oral and Parenteral Vaccines in Children, J Clin Immunol 6:43-49.

23.

Smith, D.J. ; Taubman, M.A. ; and Ebersole, J.L. (1979): Preparation of Glucosyltransferase from Streptococcus mutans by Elution from Water-insoluble Polysaccharide with a Dissociating Solvent, Infect Immun 23:446-452.

24.

Smith, D.J. ; Taubman, M.A. ; and Ebersole, J.L. (1983): Antigenic Relatedness of Glucosyltransferases from Streptococcus mutans and Streptococcus sanguis. In: Glucosyltransferases, Sucrose, Glucans and Dental Caries, R.J. Doyle and J.E. Ciardi , Eds., Sp. Supp., Chemical Senses, Washington, DC: Information Retrieval, pp. 223-230.

25.

Smith, D.J. ; Taubman, M.A. ; and Ebersole, J.L. (1985): Salivary IgA Antibody to Glucosyltransferase in Man, Clin Exp Immunol 61:416-424.

26.

Socransky, S. ; Smith, C. ; and Manganello, A. (1973): Defined Media for the Cultivation of Oral Gram-positive Rods, J Dent Res 52:88.

27.

Stiehm, E.R. and Fudenberg, H.H. (1966): Serum Levels of Immune Globulins in Health and Disease: A Survey, Pediatrics 37:715.

28.

Taubman, M.A. and Smith, D.J. (1977): Effects of Local Immunization with Glucosyltransferase Fractions from Streptococcus mutans on Dental Caries in Rats and Hamsters , J Immunol 118:710-720.

29.

Taubman, M.A. ; Smith, D.J. ; Ebersole, J.L. ; and Hill-Man, J.D. (1982): Immunological Interference with Accumulation of Cariogenic Microorganisms on Tooth Surfaces. In: Recent Advances in Mucosal Immunity, W. Strober , L. Hanson , and K. Sell , Eds., New York: Raven Press , pp. 371-382.

30.

Virella, G. ; Fudenberg, H.H. ; Kyong, K.U. ; Pandey, J.P. ; and Galbraith, R.M. (1978): A Practical Method for Quantitation of Antibody Responses to Tetanus and Diphtheria Toxoids in Normal Young Children, Z Immun Immunobiol 118 :710-720.

31.

Wittenberger, C.L. ; Beaman, A.J. ; and Lee, L.N. (1978): Tween 80 Effect on Glucosyltransferase Synthesis by Streptococcus salivarius, J Bact 133:231-239.