Abrasion Biopsy in Studies of Mineral Density of Experimental Enamel Lesions

Abstract

The profile of mineral density of experimental enamel lesions was determined by means of sampling with a new abrasion biopsy technique. Three types of lesions were produced on bovine enamel slabs: (1) pre-softened in lactic acid buffer for 16 hr; (2) pre-softened and exposed to a test for acid resistance for seven days; and (3) pre-softened, exposed to the seven-day Intra-oral Cariogenicity Test with extra-oral immersions in 1000 ppm F solutions for one min twice daily (ICT/F), plus the seven-day test for acid resistance. Lesions were assessed with measurements of surface microhardness, microradiography of thin sections, and abrasion biopsy. For abrasion biopsy of the experzmental lesions, 15 parallel layers of approximately 10 μm each were abraded simultaneously with reference slabs of sound enamel on strips of lapping film. The depth of abrasion for each sample was calculated from the phosphorus content of the reference sample. Exposure to the ICT/F formed an acid-resistant zone within the lesion which diminished the microhardness change, although it did not have an appreciable effect on the total lesion depth as assessed with microradiography; abrasion biopsy indicated the formation of a mineral-dense zone within the lesion. This recently-developed technique of abrasion biopsy of experimental lesions offers the opportunity to link the composition of the lesion to controlled experimental conditions which will improve our understanding of demineralizing and remineralizing reactions on a standard tooth substrate.

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References

Angmar, B. ; Carlstrom, D. ; and Glass, J.E. (1963): Studies on the Ultrastructure of Dental Enamel. IV. The Mineralization of Normal Human Enamel, J Ultrastruct Res 8:12-23.

Armstrong, W.D. and Brekhus, P.J. (1937): Chemical Constitution of Enamel and Dentin, J Biol Chem 120:677-687.

Borsboom, P.C.F. ; Mei, H.C.V.D. ; and Arends, J. (1985): Enamel Lesion Formation with and without 0.12 ppm F in Solution, Caries Res 19:396-402.

Brudevold, F. ; Gardner, D.E. ; and Smith, F.A. (1956): The Distribution of Fluoride in Human Enamel, J Dent Res 35 :420-429.

Brudevold, F. ; McCann, H.G. ; Nilsson, R. ; Richard-Son, B. ; and Coklica, V. (1967): The Chemistry of Caries Inhibition, Problems and Challenges of Topical Fluorides, J Dent Res 46:37-45.

Charlton, G. ; Blainey, B. ; and Schamschula, R.G. (1974): Associations between Dental Plaque and Fluoride in Human Surface Enamel, Arch Oral Biol 19:139-143.

Chen, P.S., Jr. ; Toribara, T.Y. ; and Warner, H. (1956): Microdetermination of Phosphorus , Anal Chem 28:1756-1758.

Corpron, R.E. ; More, F.C. ; Clark, J.W. ; Korytnicki, D. ; and Kowalski, C.J. (1986): In vitro Remineralization of Artificial Enamel Lesions by a Fluoride Dentifrice or Mouthrinse, Caries Res 20:48-55.

Crabb, H.S.M. (1966): Observations on the Histopathology of Carious Attack on Enamel and Related Developmental Faults. In: Advances in Fluoride Research: Dental Caries Preview, Vol. 4, Oxford : Pergamon Press, pp. 225-238.

10.

Driessens, F.C.M. ; Theuns, H.M. ; Heijligers, H.J.M. ; and Borggreven, J.M. (1986): Microradiography and Electron Microprobe Analysis of Some Natural White and Brown Spot Enamel Lesions with and without Laminations, Caries Res 20 :398-405.

11.

Feagin, F.F. ; Patel, P.R. ; Koulourides, T. ; and Pigman, W. (1971): Study of the Effect of Calcium, Phosphate, Fluoride and Hydrogen Ion Concentrations on the Remineralization of Partially Demineralized Human and Bovine Enamel Surfaces , Arch Oral Biol 16:535-548.

12.

Gerould, C.H. (1945): Electron Microscopic Study of the Mechanism of Fluorine Deposition in Teeth, J Dent Res 24:223-233.

13.

Gustafsson, G. (1957): The Histopathology of Caries of Human Dental Enamel: With Special Reference to the Division of the Carious Lesion into Zones, Acta Odontol Scand 15:13-55.

14.

Hallsworth, A.S. ; Robinson, C. ; and Weatherell, J.A. (1972): Mineral and Magnesium Distribution within the Approximal Carious Lesion of Dental Enamel, Caries Res 6:156-158.

15.

Joyston-Bechal, S. and Kidd, E.A.M. (1980): Histopathological Appearance of Artificially Produced Caries-like Lesions of Enamel Treated with APF during Lesion Formation in vitro, Caries Res 14:45-49.

16.

Kostlan, J. (1962): Translucent Zones in the Central Part of the Carious Lesion in Enamel, Br Dent J 113:244-248.

17.

Koulourides, T. (1968): Experimental Changes of Enamel Mineral Density . In: Art and Science of Dental Caries Research, R. S. Harris , Ed., New York: Academic Press, Inc., pp. 355-378.

18.

Koulourides, T. (1977): Effect of 1 ppm Fluoride on the Microradiographic Profile of Experimental Enamel Lesions, J Dent Res 56 (Sp Iss A): No. 269.

19.

Koulourides, T. (1983): Increasing Tooth Resistance to Caries through Remineralization. In: Third Annual Conference on Foods, Nutrition, and Dental Health, Vol. 2, J. J. Hefferren and H. M. Koehler , Eds., Chicago: American Dental Association, pp. 193-207.

20.

Koulourides, T. and Housch, T. (1983): Hardness Testing and Microradiography of Enamel in Relation to Intraoral De- and Remineralization. In: Demineralization and Remineralization of the Teeth, S. A. Leach and W. M. Edgar , Eds., Oxford: IRL Press, pp. 255-272.

21.

Koulourides, T. ; Keller, S.E. ; Manson-Hing, L. ; and Lilley, V. (1980): Enhancement of Fluoride Effectiveness by Experimental Cariogenic Priming of Human Enamel, Caries Res 14:32-39.

22.

Koulourides, T. ; Phantumvanit, P. ; Munksgaard, E.C. ; and Housch, T. (1974): An Intraoral Model for Studies of Fluoride Incorporation in Enamel, J Oral Pathol 3:185-196.

23.

Larsen, M.J. (1973): Dissolution of Enamel, Scand J Dent Res 81:518-522.

24.

Little, M.F. ; Posen, J. ; and Singer, L. (1962): Chemical and Physical Properties of Altered and Sound Enamel. 3. Fluoride and Sodium Content, J Dent Res 41:784-789.

25.

Manly, R.S. ; Hodge, H. ; and Ange, L.E. (1939): Density and Refractive Index Studies of Dental Hard Tissues. II. Density Distribution Curves, J Dent Res 18:203-211.

26.

Myers, H.M. ; Hamilton, J.G. ; and Becks, H. (1952): A Tracer Study of the Transfer of 18F to Teeth by Topical Application, J Dent Res 31:743-750.

27.

Patterson, C.M. ; Weatherell, J.A. ; and Robinson, C. (1984): Sampling of Porous Hard Tissues in vitro by Acid Etching, Caries Res 18:231-236.

28.

Pearce, E.I.F. (1983): A Microradiographic and Chemical Comparison of in vitro Systems for the Simulation of Incipient Caries in Abraded Bovine Enamel, J Dent Res 62:969-974.

29.

Silverstone, L.M. (1970): The Histopathology of Early Caries in the Enamel of Primary Teeth, J Dent Res 37:17-27.

30.

Silverstone, L.M. (1973): Structure of Carious Enamel Including the Early Lesion, Oral Sci Rev 3:100-160.

31.

Ten Cate, J.M. and Duijsters, P.P.E. (1983): Influence of Fluoride in Solution on Tooth Demineralization. II. Microradiographic Data, Caries Res 17:513-519.

32.

Ten Cate, J.M. and Rempt, H.E. (1986): Comparison of the in vivo Effect of a 0 and 1,500 ppm F MFP Toothpaste on Fluoride Uptake, Acid Resistance and Lesion Remineralization , Caries Res 20:193-201.

33.

Theuns, H.M. ; Driessens, F.C.M. ; and Van Dijk, J.W.E. (1986): Effect of Under- and Supersaturation with Respect to Some Apatites in Demineralizing Buffers on Artificial Lesion Formation in Human Tooth Enamel, Caries Res 20:315-323.

34.

Thurlow, S. and Bunzel, H.H. (1927): Tooth Decay Studies, Dental Cosmos 69:20-35.

35.

Weatherell, J.A. ; Weidman, S.M. ; and Hamm, S.M. (1966): Sampling of Enamel Particles by Means of Strong Acids for Density Measurements, Arch Oral Biol 11:107-111.

36.

Weatherell, J.A. ; Robinson, C. ; Strong, M. ; and Na-Kagaki, H. (1985): Micro-sampling by Abrasion, Caries Res 19:97-102.

37.

Winer, B.J. (1971): Statistical Principles in Experimental Design , 2nd ed., New York: McGraw-Hill, p. 201.