Sage Journals: Discover world-class research

Abstract

Small samples of hydroxyapatite (HA) beads were coated with 10 μL of either saliva, serum, human crevicular fluid, or a preparation of lysosomal enzymes from human polymorphonuclear leucocytes (PMN), before being added to suspensions of Treponema denticola. The beads were then observed with the scanning electron microscope.

Abundant T. denticola were found to adhere to HA or HA coated with saliva, serum, or crevicular fluid. Coating the beads with lysosomal enzymes consistently prevented the adhesion of T. denticola. Adhesion of treponemes to hard surfaces, modulated by enzymes from PMNs, could represent a new virulence factor for these bacteria.

Get full access to this article

View all access options for this article.

References

Baehni, P. (1986): Interactions between Plaque Microorganisms and Human Oral Epithelial Cells. In: Borderland between Caries and Periodontal Disease. 3rd European Symposium, T. Lehner and G. Cimasoni , Eds., Geneva: Médécine et Hygiene, pp. 143-153.

Carrassi, A. (1986): The of Subgingival Plaque in Rapidly Progressive Periodontitis. In: Borderland between Caries and Periodontal Disease- 3rd European Symposium, T. Lehner and G. Cimasoni , Eds., Geneva: Médicine et Hygiene, pp. 377-382.

Cimasoni, G. (1983): Crevicular Fluid Updated. In: Monographs in Oral Science, H. Myers , Ed., Basel: Karger.

Cimasoni, G. ; Song, M. ; and McBride, B.C. (1987): Effect of Crevicular Fluid and Lysosomal Enzymes on the Adherence of and Bacteroides to Hydroxyapatite, Infect Immun 55:1484-1489.

Dawson, R.N. ,C.; Elliott, D.C. ; Elliott, W.H. ; and Jones, K.N. (1969): Data for Biochemical Research, Oxford: Clarendon Press, pp. 625-626.

Fitzgerald, T.J. (1981): Pathogenesis and Immunology of Treponema pallidum , Ann Rev Microbiol 35:29-54.

Frank, R.M. (1980): Bacterial in the Apical Pocket Wall of Advanced Human Periodontitis, J Periodont Res 15:563-573.

Laughon, B.E. ; Syed, S.A. ; and Loesche, W.J. (1982): API ZYM System for Identification of Bacteroides spp., Capnocytophaga spp., and Spirochetes of Oral Origin, Infect Immun 15:97- 102.

Listgarten, M. and Hellden, L. (1978): Relative Distribution of Bacteria at Clinically Healthy and Periodontally Diseased Sites in Humans, J Clin Periodontol 5:115-132.

10.

Loesche, W.J. and Laughon, B.C. (1982): Rote of Spirochetes in Periodontal Diseases, In: Host-Parasite Interactions in Periodontal Diseases, R.J. Genco and S.E. Mergenhagen , Eds-, Washington, D.C.: American Society for Microbiology, pp. 62-75.

11.

McBride, B.C. ; Morris, E.J. ; and Ganeshkumar, N. (1985): Relationships of Streptococcal Cell Surface Proteins to Hydrophobicity and Adherence. In: Molecular Basis of Oral Microbial Adhesion, S.E. and B. Rosan , Eds., Washington, D.C.: American Society for Microbiology, pp. 85-93.

12.

Olsen, J. (1984): Attachment of Treponema denticola to Cultured Human Epithelial Cells, Scand J Dent Res 92-55-63.

13.

Reijntjens, F.M. ; Mikx, F.H. ; Wolters-Lutgeriiorst, J.M. -; and Maltha, J.C. (1986): Adherence of Oral Treponemes and Their Effect on Morphological Damage and Detachment of Epithelial Cells in vitro, Infect Immun 51:642-647.

14.

Skoog, W.A. and Beck, W.S. (1956): Studies on the Fibrinogen, Dextran and Phytohemagglutinin Methods of Isolating Leukocytes, Blood 11:436-454.

15.

Sueda, T. ; Bang, J. ; and Gimasoni, G. (1969): Collection of Gingival Fluid for Quantitative Analysis, J Dent Res 48:159.

16.

Virca, G. ; Metz, G. ; and Schneibli, H.P. (1984): Similarities Between Human and Rat Leucocyte Elastase and Cathepsin G, Eur J Biochem 144:1-9.

Adherence of Treponema denticola to Modified Hydroxyapatite

Abstract

Get full access to this article

References