Dentin matrix was assayed for its potential to elicit chondrogenesis of mesenchymal cells in vitro. The substratum was prepared by demineralization of human tooth root dentin, while embryonic thigh muscle was used as a source of mesenchymal cells. Formation of chondrocytes from mesenchymal cells occurred in the presence of dentin matrix, and in the same sequence as previously shown with substrata of demineralized bone.
Get full access to this article
View all access options for this article.
References
1.
Bang, G.; Nordenram, A.; and Anneroth, G. (1972): Allogenic Demineralized Dentin Implants in Jaw Defects of Java Monkeys, Int J Oral Surg1:126-136.
2.
Bang, G. and Urist, M.R. (1967): Bone Induction in Excavation Chambers in Matrix of Decalcified Dentin, Arch Surg94 :781-789.
3.
Everett, F.G. and Bruckner, M.S. (1970): Cartilage in the Periodontal Ligament Space, J Periodontol41:165-169.
4.
Glimcher, M.J.; Kossiva, D.; and Brickley-Parsons, D. (1984): Phosphoproteins of Chicken Bone Matrix: Proof of Synthesis in Bone Tissue, J Biol Chem259:290-293.
5.
Huggins, C.B. and Urist, M.R. (1970): Dentin Matrix Transformation : Rapid Induction of Alkaline Phosphatase and Cartilage, Science167:896-897.
6.
Inoue, T.; Deporter, D.A.; and Melcher, A.H. (1986): Induction of Chondrogenesis in Muscle, Skin, Bone Marrow and Periodontal Ligament by Demineralized Dentin and Bone Matrix in vivo and in vitro, J Dent Res65:12-22.
7.
Johnson, D.G. and deC. Nogueira-Araujo, G.M. (1981): A Simple Method of Reducing the Fading of Immunofluorescence During Microscopy, J Immunol Meth43:349-356.
8.
Laskey, R.A. and Mills, A.D. (1975): Quantitative Film Detection of 3H and 14C in Polyacrylamide Gels by Fluorography, Eur J Biochem56:325-341.
9.
Lee, S.L.; Veis, A.; and Glonek, I. (1977): Dentin Phosphoprotein : An Extracellular Calcium-binding Protein, Biochem16:2971-2979.
10.
Libin, B.M.; Ward, H.L.; and Fishman, L. (1975): Decalcified, Lyophilized Bone Allografts for Use in Human Periodontal Defects, J Periodontol46:51-56.
11.
Lindhe, A.; Brown, M.; and Butler, W.I. (1981): Non-collagenous Proteins of Rat Dentin. Evidence That Phosphoprotein is not Covalently Bound to Collagen, Biochim Biophys Acta667 :341-350.
12.
Nathanson, M.A. (1983): Proteoglycan Synthesis by Skeletal Muscle Undergoing Bone Matrix-directed Transformation into Cartilage in vitro, J Biol Chem158:10325-10334.
13.
Nathanson, M.A. and Hay, E.D. (1980a): Analysis of Cartilage Differentiation from Skeletal Muscle Grown on Bone Matrix. I. Ultrastructural Aspects, Dev Biol78:301-331a.
14.
Nathanson, M.A. and Hay, E.D. (1980b): Analysis of Cartilage Differentiation from Skeletal Muscle Grown on Bone Matrix. II. Chondroitin Sulfate Synthesis and Reaction to Exogenous Glycosaminoglycans, Dev Biol78:332-351b.
15.
Nathanson, M.A.; Hilfer, S.R.; and Searls, R.L. (1978): Formation of Cartilage by Non-chondrogenic Cell Types, Dev Biol64:99-117.
16.
Nogami, H. and Urist, M.R. (1974): Substrata Prepared from Bone Matrix for Chondrogenesis in Tissue Culture, J Cell Biol62:510-519.
17.
Prince, C.W.; Oosawa, T.; Butler, W.T.; Tomama, M.; Bhown, A.S.; Bhown, M.; and Schrohenloher, R.E. (1987): Isolation, Characterization and Biosynthesis of a Phosphorylated Glycoprotein from Rat Bone, J Biol Chem262 :2900-2907.
18.
Reddi, A.H. (1981): Cell Biology and Biochemistry of Endochondral Bone Development, Coll Rel Res1:209-229.
19.
Reddi, A.H. and Huggins, C.B. (1972): Biochemical Sequences in the Transformation of Normal Fibroblasts in Adolescent Rats, Proc Natl Acad Sci USA69:1601-1605.
20.
Sampath, I.K.; Nathanson, M.A.; and Reddi, A.H. (1984): In vitro Transformation of Mesenchymal Cells Derived from Embryonic Muscle into Cartilage in Response to Extracellular Matrix Components of Bone, Proc Natl Acad Sci USA81:3419-3423.
21.
Sanders, J.J.; Sepe, W.W.; Bowers, G.M.; Koch, R.W.; Williams, J.E.; Lekas, J.S.; Mellonig, J.I.; Pelleu, G.B.; and Gambill, V. (1982): Clinical Evaluation of Freeze-dried Bone Allografts in Periodontal Osseous Defects. III. Composite Freeze-dried Bone Allografts With and Without Autogenous Bone Grafts, J Periodontol54:1-8.
22.
Sauk, J. (1985): Basement Membrane Confinement of Epithelial Tumor Islands in Benign and Malignant Ameloblastomas, J Oral Pathol14:307-314.
23.
Seyedin, S.M.; Thomas, T.C.; Thompson, A.Y.; Rosen, D.M.; and Piez, L.A. (1985): Purification and Characterization of Two Cartilage-inducing Factors from Bovine Demineralized Bone, Proc Natl Acad Sci USA82:2267-2271.
24.
Syftestad, G.T.; Triffitt, J.T.; Urist, M.R.; and Caplan, A.I. (1984): An Osteo-inductive Bone Matrix Extract Stimulates the in vitro Conversion of Mesenchyme into Chondrocytes, Calcif Tissue Int36:625-627.
25.
Timpl, R.; Martin, G.R.; Bruckner, P.; Wick, G.; and Wei-Demann, H. (1978): Nature of the Collagenous Protein in Tumor Basement Membrane, Eur J Biochem84:43-52.
26.
Urist, M.R. (1965): Bone: Formation by Autoinduction, Science150:893-899.
27.
Urist, M.R. (1971): Bone Histogenesis and Morphogenesis in Implants of Demineralized Enamel and Dentin, J Oral Surg29 :88-102.
28.
Urist, M.R.; Hou, Y.K.; Brownell, A.G.; Hah, W.M.; Buyske, J.; Lietze, A.; Tempst, P.; Hunkapiller, M.; and Delange, R.J. (1984): Purification of Bovine Bone Morphogenetic Protein by Hydroxyapatite Chromatography, Proc Natl Acad Sci USA81:371-375.
29.
Yoemans, J.D. and Urist, M.R. (1967): Bone Induction by Decalcified Dentin Implanted into Oral and Muscle Tissues, Arch Oral Biol12:999-1008.
