Effect of β2-Adrenoceptor Agonists on Saliva Proteins and Dental Caries in Asthmatic Children

Abstract

Twenty-four children, from 10 to 20 years old, with asthma treated with β₂-adrenoceptor agonists were matched with healthy controls of the same age, sex, and social background. Stimulated whole and parotid saliva was collected, and decayed and filled tooth surfaces as well as oral hygiene habits were recorded. The dietary and sugar intake was carefully checked by a four-day dietary record. The asthmatic children had a 26% lower (p<0.05) value for secretion rate of whole saliva. Seventy percent of the children with Streptococcus mutans counts >2×10⁵ colony-forming units/mL of whole saliva belonged to the asthmatic group (p<0.05). The concentrations of total protein and amylase in parotid saliva were significantly lower for the asthmatic children. The concentrations of potassium, salivary peroxidase, bacteria-aggregating glycoproteins, and secretory IgA were not affected, but the secretion rate of parotid saliva was 36% lower in the asthma group (p<0.05). Oral hygiene and dietary habits did not differ between the groups. The asthmatic children had higher DFS scores, but these were not significantly different from those of the healthy controls (p = 0.07). We suggest that subjects with asthma treated with β₂-receptor agonists should receive special prophylactic attention.

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References

Ainamo, J. and Bay, I. (1976): Problems and Proposals for Recording Gingivitis and Plaque, Int Dent J 25:229-235.

Blix, G. (1948): The Determination of Hexosamine According to Elson and Morgan, Acta Chem Scand 2:467-473.

Brandtzaeg, P. (1971): Human Secretory Immunoglobulins. VII. Concentrations of Parotid IgA and Other Secretory Proteins in Relation to the Rate of Flow and Duration of Secretory Stimulus, Arch Oral Biol 16:1295-1310.

Brasher, G.W. (1971): Salivary IgA in Children with Atopic Diseases , Ann Allergy 29:422-427.

Bylund, D.B. ; Martinez, J.R. ; and Pierce, D.L. (1982): Regulation of Autonomic Receptors in Rat Submandibular Gland, Molec Pharmacol 21:27-35.

Carlsoo, B. ; Danielsson, A. ; Henriksson, R. ; and Idahl, L.-A. (1981): Characterization of the Rat Parotid β-Adrenoceptor , Br J Pharmacol 72:271-276.

Conolly, M.E. and Greenacre, J.K. (1976): The Lymphocyte β-Adrenoceptor in Normal Subjects and Patients with Bronchial Asthma, J Clin Invest 58:1307-1316.

Crespi, H. ; Armando, I. ; Tumilasci, 0.; Levin, G. ; Mas-Simo, J. ; Barontini, M. ; and Perec, C. (1982): Catecholamines Levels and Parotid Secretion in Children with Chronic Atopic Dermatitis, J Invest Dermatol 78:493-497.

Ericson, T. ; Pruitt, K. ; and Wedel, H. (1975): The Reaction of Salivary Substances with Bacteria , J Oral Pathol 4:307-323.

10.

Ericsson, Y. (1959): Clinical Investigations on the Salivary Buffering Action, Acta Odontol Scand 17:131-165.

11.

Gibbons, R.J. and Van Houte, J. (1975): Bacterial Adherence in Oral Microbial Ecology , Ann Rev Microbiol 29:19-44.

12.

Gothefors, L. and Marklund, S. (1975): Lactoperoxidase Activity in Human Milk and Saliva of Newborn Infants, Infect Immun 11:1210-1215.

13.

Gozal, D. ; Szargel, R. ; Ben-Aryeh, H. ; and Colin, A. (1985): Salivary Composition in Asthmatic Children on Theophylline, Isr J Med Sci 21:460-461.

14.

Henriksson, R. (1981): β1- and β2-Adrenoceptor Agonists have Different Effects on Rat Parotid Acinar Cells, Am J Physiol 242:G481-G485.

15.

Hyyppa, T. (1980): Salivary Immunoglobulins in Children with Asthma , J Periodont Res 15 :227-231.

16.

Hyyppa, T. (1981) : Studies on Immunologic and Inflammatory Factors in Saliva in Patients with Asthma and in Patients with Periodontitis, J Clin Periodontol 8:500-507.

17.

Hyyppa, T. and Paunio, K. (1979): Oral Health and Salivary Factors in Children with Asthma, Proc Finn Dent Soc 75:7-10.

18.

Inoue, T. ; Shimono, M. ; and Yamamura, T. (1980): Unusual Lamellar Inclusions in Secretory Granules of the Rat Submandibular Gland Induced by Long-term Repeated Administration of Isoproterenol, J Electron Microsc 29:261-265.

19.

Johnson, D.A. (1983): Differences in Basic Proline-rich Proteins in Rat Parotid Saliva Following Chronic Isoproterenol Treatment or Maintenance on a Liquid Diet, Arch Oral Biol 28:549-554.

20.

Lenox, J.A. and Kopczyk, R.A. (1973): A Clinical System for Scoring a Patient's Oral Hygiene Performance, J Am Dent Assoc 86:849-852.

21.

Magnusson, I. ; Ericson, T. ; and Pruitt, K. (1976): Effect of Salivary Agglutinins on Bacterial Colonization of Tooth Surfaces, Caries Res 10:113-122.

22.

Mednieks, M.I. and Hand, A.R. (1984): Microheterogeneity of Rat Parotid Gland Proteins After Chronic Treatment with Isoproterenol, J Dent Res 63:87-93.

23.

Menaker, L. and Navia, J.M. (1975): Caries Incidence in Well-fed and Malnourished Rats Treated with Isoproterenol during Development, Caries Res 9:83-90.

24.

Miklich, D.R. (1974): Salivary Flow as Measures of Parasympathetic Nervous System Involvement in Children's Asthma, Psychosom Med 36:206-214.

25.

Quissell, D.O. ; Deisher, L.M. ; and Barzen, K.A. (1983): Role of Protein Phosphorylation in Regulating Rat Submandibular Mucin Secretion, Am J Physiol 245:G44-G53.

26.

Schneyer, C.A. (1978): Retardation of Secretory Capacity of Immature Rat Parotid Gland by Chronic Administration of Isoproterenol, Pediat Res 12:726-728.

27.

Schneyer, C.A. (1985): Calcium and Norepinephrine Levels of Rat Salivary Glands After Terbutaline, Dobutamine or Isoproterenol, J Auton Nerv Syst 14:191-200.

28.

Shannon, I.L. and Suddick, R.P. (1975): Current Research on Oral Fluids, J Dent Res 54 (Sp Issue B):B8-B18.

29.

Siegel, S. (1956): Nonparametric Statistics for the Behavioural Sciences , New York: McGraw-Hill Co., Inc .

30.

Sodicoff, M. ; Sinesi, M.S. ; and Pratt, N.E. (1980): Terbutaline-induced Enlargement of the Rat Parotid Gland, Arch Oral Biol 25:781-783.

31.

Spector, T. (1978): Refinement of the Coomassie Blue Method of Protein Quantitation, Anal Biochem 86:142-146.

32.

Szentivanyi, A. (1980): The Radioligand Binding Approach in the Study of Lymphocytic Adrenoceptors and the Constitutional Basis of Atopy, J Allergy Clin Immunol 65:5-11.

33.

Van Houte, J. and Green, D.B. (1974): Relationship Between the Concentration of Bacteria in Saliva and the Colonization of Teeth in Humans, Infect Immun 9:624-630.

34.

Venter, J.C. ; Fraser, C.M. ; and Harrison, L.C. (1980): Autoantibodies to β2-Adrenergic Receptors: A Possible Cause of Adrenergic Hyporesponsiveness in Allergic Rhinitis and Asthma, Science 207:1361-1363.

35.

Venter, J.C. ; Fraser, C.M. ; Harrison, L.C. ; and Kali-Ner, M. (1981): Autoantibodies to β2-Adrenergic Receptors Correlate with Autonomic Nervous System Abnormalities, Fed Proc 40:355 (abstr 697).