Sage Journals: Discover world-class research

Abstract

In this study, the effects of eugenol on human polymorphonuclear (PMN) cell migration and chemiluminescence were examined in vitro. Utilizing zymosan-activated serum or crude Bacteroides sonicate fractions as chemotractants, we found that eugenol inhibits PMN migration at 6.6 x 10 ^-2 to 6.6 x 10 ^-5 mol/L (P<0.05). Also, similar effects were observed in PMNs pre-incubated in eugenol. Regardless of concentration, eugenol was not found to induce chemotaxis of PMNs. An examination of PMN membrane activation through chemiluminescence gave results consistent with the chemotaxis data, demonstrating a decrease in light emission at concentrations as low as 6.6 x 10-6 mol/L (P<0.05). In view of these data, the potential effect of eugenol on in vivo (sulcular or periapical) PMN function deserves further study.

Get full access to this article

View all access options for this article.

References

Barkin, M.E. ; Boyd, J.P. ; and Cohen, S. (1984): Acute Allergic Reaction to Eugenol, Oral Surg 57:441-442.

Barnett, F. ; Duran, C. ; Hasselgren, G. ; and Tronstad, L. (1984): Tissue Response to Anodyne Medicaments, Oral Surg 8:605-609.

Grossman, L.I. and Lally, E.T. (1982): Assessment of Irritation Potential of Essential Oils for Root Canal Cement, J Endodont 8:208-212.

Horan, T.D. ; English, D. ; and McPherson, T.A. (1982): Association of Neutrophil Chemiluminescence with Microbicidal Activity, Immunol Immunopathol 22:259-269.

Hume, W.R. (1983): Effect of Eugenol on Constrictor Responses in Blood Vessels of the Rabbit Ear, J Dent Res 62:1013-1015.

Hume, W.R. (1984a): An Analysis of the Release and Diffusion Through Dentin of Eugenol from Zinc Oxide-Eugenol Mixtures, J Dent Res 63:881-884.

Hume, W.R. (1984b): Effect of Eugenol on Respiration and Division in Human Pulp, Mouse Fibroblasts, and Liver Cells in vitro, J Dent Res 63:1262-1265.

Issacs, G. (1983): Permanent Local Anaesthesia and Anhydrosis After Clove Oil Spillage, Lancet 16:882.

Kozam, G. (1977): The Effect of Eugenol on Nerve Transmission, Oral Surg 44:799-805.

10.

Larsen, W.G. (1985): Perfume Dermatitis, J Am Acad Dermatol 12:1-9.

11.

Linqvist, L. and Otteskog, P. (1981): Eugenol: Liberation from Dental Materials and Effect on Human Diploid Fibroblast Cells, Scand J Dent Res 89:552-556.

12.

Nelson, R.D. ; Quie, P.G. ; and Simmons, T.L. (1975): Chemotaxis Under Agarose: A New and Simple Method for Measuring Chemotaxis and Migration of Human Polymorphonuclear Leukocytes and Monocytes, J Immunol 115:1650.

13.

Rasheed, A. ; Laekman, G. ; Totte, J. ; and Vlietinck, A.J. (1984): Eugenol and Prostaglandin Biosynthesis, N Engl J Med 310:50-51.

14.

Trowbridge, H. ; Edwall, L. ; and Panopoulos, P. (1982): Effect of Zinc Oxide-Eugenol and Calcium Hydroxide on Intra-dental Nerve Activity, J Endodont 8:403-406.

15.

Woolverton, C.J. and Fotos, P.G. (1986): Mutagenic Effect of Eugenol Utilizing the Mouse Micronucleus Test, J Dent Res 65:575.

Effects of Eugenol on Polymorphonuclear Cell Migration and Chemiluminescence

Abstract

Get full access to this article

References