T6 antigen is a highly specific marker for human Langerhans cells (LC). Previous studies have demonstrated that Interleukin-1 (IL-1) and an IL-1 inhibitor (ILS) modulate LC T6 expression (T6E) in explant culture. The present study examined the in vitro modulation of T6E by two molecules: epidermal-cell-derived thymocyte-activating factor (ETAF), and a bone-derived protein (BP) implicated in the control of bone homeostasis. The effect of purified ILS was also examined. ETAF mimicked the stimulatory effect of IL-1 on LC T6E, while BP depressed T6E in a manner resembling that seen with ILS. No agents altered Class II (DR and DQ) expression by LC. BP was a specific IL-1 inhibitor, and did not inhibit thymocyte proliferation in the standard IL-1 bioassay in the absence of IL-1. These results demonstrate that molecules resembling IL-1 or ILS can modulate T6E, and implicate locally produced ETAF and ILS in the regulation of T6E in the oral mucosa in vivo.
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References
1.
Bergstresser, P.R.; Toews, G.B.; and Streilein, J.W. (1980): Natural and Perturbed Distributions of Langerhans Cells: Responses to Ultraviolet Light, Heterotopic Skin Grafting, and Dinitrofluorobenzene Sensitization, J Invest Dermatol75:73-77.
2.
Dewhirst, F.E.; Stashenko, P.P.; Mole, J.E.; and Tsu-Rumachi, T. (1985): Purification and Partial Sequence of Human Osteoclast Activating Factor: Identity with Interleukin 1, J Immunol135:2562-2568.
3.
Gowen, M.; Wood, D.D.; Thrie, E.J.; McGuire, M.K.B.; and Russell, R.G.G. (1983): An Interleukin I Like Factor Stimulates Bone Resorption in vitro, Nature306:378-380.
4.
Lander, P.E.; Seymour, G.J.; Masci, P.; and Powell, R.N. (1986): The Extraction and Purification of a Murine Bone Regulatory Protein, J Dent Res65:479, Abst. No. 69.
5.
Liao, Z.; Grimshaw, R.S.; and Rosenstreich, D.L. (1984): Identification of a Specific Interleukin-1 Inhibitor in the Urine of Febrile Patients, J Exp Med159:126-136.
Luger, T.A.; Charon, J.A.; Colot, M.; Mickshe, M.; and Oppenheim, J.J. (1983): Chemotactic Properties of Partially Purified Human Epidermal Cell-derived Thymocyte-activating Factor (ETAF) for Polymorphonuclear and Mononuclear Cells, J Immunol131:816-820.
8.
Luger, T.A.; Kock, A.; and Danner, M. (1985a): Characterization of Immunoregulatory Cytokines Produced by Epidermal Cells, Scand J Immunol21:455-462.
9.
Luger, T.A.; Wirth, U.; and Kock, A. (1985b): Epidermal Cells Synthesize a Cytokine with Interleukin 3-like Properties, J Immunol134 :915-919.
10.
Mannel, D.N.; Mizel, S.B.; Diamenstein, T.; and Falk, W. (1985): Induction of Interleukin 2 Responsiveness in Thymocytes by Synergistic Action of Interleukin-1 and Interleukin-2, J Immunol134:3108-3110.
11.
Mizel, S.B.; Oppenheim, J.J.; and Rosenstreich, D.L. (1978): Characterization of Lymphocyte-activating Factor (LAF) Produced by a Macrophage Cell Line, P388D1. II. Biochemical Characterization of LAF Induced by Activated T Cells and LPS, J Immunol120:1504-1508.
12.
Murphy, G.F.; Bhan, A.K.; Sato, S.; Harrist, T.J.; and Mihm, M.C. (1981): Characterization of Langerhans Cells by the Use of Monoclonal Antibodies, Lab Invest45:465-468.
Oppenheim, J.J.; Kovacs, E.J.; Matsushima, K.; and Durum, S.K. (1986): There is More Than One Interleukin 1, Immunol Today7:45-56.
15.
Sauder, D.N.; Dinarello, C.A.; and Morhenn, V.B. (1984): Langerhans Cell Production of Interleukin-1, J Invest Dermatol82:605-607.
16.
Scala, G.; Kuang, Y.D.; Hall, R.E.; Muchmore, A.V.; and Oppenheim, J.J. (1984): Accessory Cell Function of Human B Cells. I. Production of Both Interleukin 1-like Activity and an Interleukin 1 Inhibitory Factor by an EBV-Transformed Human B Cell Line, J Exp Med159:1637-1652.
17.
Smith, K.A.; Lachman, L.B.; Oppenheim, J.J.; and Favata, M.F. (1980): The Functional Relationship of the Interleukins , J Exp Med151:1551-1556.
18.
Stingl, G.; Katz, S.I.; Green, I.; and Shevach, E.M. (1980): The Functional Role of Langerhans Cells, J Invest Dermatol74:315-318.
19.
Walsh, L.H.; Powell, R.N.; Seymour, G.J.; and New-Comb, G.M. (1984): Loss of Langerhans Cells from Gingival Tissue Maintained in Organ Culture, J Oral Pathol13:604-613.
20.
Walsh, L.J.; Seymour, G.J.; and Powell, R.N. (1985): The in vitro Effect of Retinol on Human Gingival Epithelium. II. Modulation of Langerhans Cell Markers and Interleukin-1 Production , J Invest Dermatol85:501-506.
21.
Walsh, L.J.; Seymour, G.J.; and Powell, R.N. (1986a): Modulation of Gingival Langerhans Cell T6 Antigen Expression in vitro by Interleukin-1 and an IL-1 Inhibitor, Clin Exp Immunol64:334-341.
22.
Walsh, L.J.; Seymour, G.J.; and Powell, R.N. (1986b): Modulation of Langerhans Cell T6 Antigen Expression by Interleukin-1, J Dent Res65:473, Abst. No. 14.
23.
Walsh, L.J.; Seymour, G.J.; and Powell, R.N. (1986c): Modulation of Class II (DR and DQ) Antigen Expression on Gingival Langerhans Cells in vitro by Gamma Interferon and Prostaglandin E2, J Oral Pathol15:347-351.
24.
Walsh, L.K.; Seymour, G.J.; and Savage, N.W. (1986d): Oral Mucosal Langerhans Cells Express DR and DQ Antigens, J Dent Res65:390-393.
25.
Walsh, L.J.; Seymour, G.J.; and Powell, R.N. (1986e): Interleukin-1 Modulates T6 Expression on a Putative Intraepithelial Langerhans Cell Precursor Population, J Dent Res65:1424-1426.
26.
Wynne, S.E.; Walsh, L.J.; Seymour, G.J.; and Powell, R.N. (1986): In situ Identification of Natural Killer (NK) Cells in Human Gingival Tissues, J Periodontol (in press).
