The present communication provides descriptions of the developmental, biochemical, and immunological properties of the human enamel extracellular matrix proteins. We report the isolation and partial characterization of the major human enamel proteins, the production of polyclonal antibodies directed against the human enamelins, and a comparison between the immunogenicity of enamelins and amelogenins from human and mouse enamel extracellular matrices. Our results indicate that although enamelins and amelogenins share some epitopes, each one of these proteins appears to invoke a different degree of immunogenicity.
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References
1.
Belcourt, A.B.; Fincham, A.G.; and J.D. (1983): Bovine High Molecular Weight Amelogenin Proteins, Calcif Tissue Int35:111-114.
2.
Bessem, C.; Bringas, P.; and Slavkin, H.C. (1985): Differential Gene Expression of Enamelins and Amelogenins During Mouse Molar Tooth Organogenesis, Cell Diff16 (Sp Iss): No. 309.
3.
Christner, P.J.; Lally, E.T.; Eds, A.H.; and Herold, R.C. (1983): Production of a Monoclonal Antibody to Bovine Tooth Enamel Proteins, Arch Oral Biol28:773-779.
4.
Deutsch, D. and Gedalia, I. (1980): Chemically Distinct Stages in Developing Human Fetal Enamel, Arch Oral Biol25:635-639.
5.
Deutsch, D.; Shapira, L.; Alayoff, A.; Leviel, D.; Yoeli, Z.; and Arad, A. (1984): Protein and Mineral Changes during Prenatal and Postnatal Development and Mineralization of Human Deciduous Enamel. In: Tooth Enamel IV, R.W. Feamhead and S. Suga, Eds., Amsterdam: Elsevier Science Publishers, pp. 234-239.
6.
Deutsch, D.; Vasserstein, A.; Pe'er, E.; and Asalem, A. (1983): Chemical Stages and Associated Mineral and Protein Changes in the Developing Enamel of the Human Deciduous Maxillary Central Incisor, Isr J Dent Sci1:22-31.
7.
Fincham, A.G. (1980): Changing Amino Acid Profiles of Developing Dental Enamel in Individual Human Teeth and the Comparison of the Protein Matrix of Developing Human and Bovine Enamel, Arch Oral Biol25:669-674.
8.
Fincham, A.G.; Belcourt, A.B.; Lyaruu, D.M.; and Termine, J.D. (1982): Comparative Protein Biochemistry of Developing Dental Enamel Matrix From Five Mammalian Species, Calcif Tissue Int34:182-189.
9.
Fincham, A.G.; Belcourt, A.B.; Termine, J.D.; Butler, W.T.; and Cothran, W.C. (1983a): Amelogenins: Sequence Homologies in Enamel-matrix Proteins From Three Mammalian Species, Biochem J211:149-154.
10.
Fincham, A.G.; Belcourt, A.B.; and Termine, J.D. (1983b): Molecular Composition of the Protein Matrix of Developing Human Dental Enamel, J Dent Res62:11-15.
11.
Fukae, M. and Shimizu, M. (1985): Amino Acid Sequence of a Protein From Developing Porcine Enamel, Jpn J Oral Biol25 (supplement): 29.
12.
Glimcher, M.J.; Brickley-Parsons, D.; and Levine, P.T. (1977): Studies of Enamel Proteins During Maturation, Calcif Tissue Res24:259.
13.
Gomer, R.H. and Lazarides, E.R. (1981): The Synthesis and Deployment of Filamin in Chicken Skeletal Muscle, Cell23 :524-532.
14.
Hawkes, R.; Niday, E.; and Gordon, J. (1982): A Dot-immunobinding Assay for Monoclonal and Other Antibodies, Anal Biochem119:142-147.
15.
Herold, R.; Graver, H.; and Christner, P. (1980): Immunohistochemical Localization of Amelogenins in Enameloid of Lower Vertebrate Teeth, Science207:1357-1358.
16.
Kinoshita, Y. (1979): Incorporation of Serum Albumin into the Developing Dentine and Enamel Matrix in the Rabbit Incisor, Calcif Tissue Int29:41-46.
17.
Lyaruu, D.M.; Belcourt, A.B.; Fincham, A.G.; and Termine, J.D. (1982): Neonatal Hamster Molar Tooth Development: Extraction and Characterization of Amelogenins, Enamelins and Soluble Dentin Proteins , Calcif Tissue Int34:86-96.
18.
O'Farrell, P.H. (1975): High Resolution Two-dimensional Electrophoresis of Proteins, J Biol Chem250:4007-4021.
19.
O'Farrell, P.Z.; Goodman, H.M.; and O'Farrell, P.H. (1977): High Resolution Two-dimensional Electrophoresis of Basic as Well as Acidic Proteins, Cell12:1133-1142.
20.
Robinson, C.; Lowe, N.R.; and Weatherell, J.A. (1977): Changes in Amino Acid Composition of Developing Rat Incisor Enamel, Calcif Tissue Res23:19-31.
21.
Robinson, C.; Briggs, H.D.; and Atkinson, P.J. (1981a): Histology of Enamel Organ and Chemical Composition of Adjacent Enamel in Rat Incisors, Calcif Tissue Int33:513.
22.
Robinson, C.; Briggs, H.D.; Atkinson, P.J.; and Weatherell, J.A. (1981b): Chemical Changes During Formation and Maturation of Human Deciduous Enamel, Arch Oral Biol26 :1027-1033.
23.
Robinson, C. and Kirkham, J. (1984): Enamel Matrix Components, Alterations During Development and Possible Interaction with the Mineral Phase. In: Tooth Enamel IV, R.W. Feamhead and S. Suga, Eds., Amsterdam: Elsevier Science Publishers B.V., pp. 261-265.
24.
Slavkin, H.C.; Graham, E.; Zeichner-David, M.; and Hildemann, W. (1983): Enamel-like Antigens in Hagfish: Possible Evolutionary Significance, Evolution37:404-412.
25.
Slavkin, H.C.; Zeichner-David, M.; and Siddiqui, M.A.Q. (1981): Molecular Aspects of Tooth Morphogenesis and Differentiation, Molec Aspects Med4:127.
26.
Slavkin, H.C.; Zeichner-David, M.; Macdougall, M.; Bringas, P.; Bessem, C.; and Hoing, L.S. (1982a): Antibodies to Murine Amelogenins: Localization of Enamel Proteins During Tooth Organ Development in vitro, Differentiation23 :73-82.
27.
Slavkin, H.C.; Zeichner-David, M.; and Siddiqui, M.A.Q. (1982b): Enamel Extracellular Matrix: Differentiation of Specific Gene Products and the Control of Their Synthesis and Accumulation During Development. In: Current Advances in Skeletogenesis, M. Silberman and H.C. Slavkin, Eds., Amsterdam: Elsevier Science Publishing Co., pp. 24-33.
28.
Snead, M.L.; Zeichner-David, M.; Chandra, T.; Robson, K.J.H.; Woo, S.L.C.; and Slavkin, H.C. (1983): Construction and Identification of Mouse Amelogenin cDNA Clones, Proc Natl Acad Sci USA80:7254-7258.
29.
Takagi, T.; Suzuki, M.; Baba, T.; Minegishi, K.; and Sasaki, S. (1984): Complete Amino Acid Sequence in Developing Bovine Enamel, Biochem Biophys Res Commun121:592.
30.
Termine, J.D.; Belcourt, A.B.; Christner, P.J.; Conn, K.M.; and Nylen, M. (1980): Properties of Dissociatively Extracted Fetal Tooth Matrix Proteins. I. Principal Molecular Species in Developing Bovine Enamel, J Biol Chem255:9760-9768.
31.
Thomas, T. and Leaver, A.G. (1975): Identification and Estimation of Plasma Proteins in Human Dentine, Arch Oral Biol20:217-218.
32.
Towbin, H.; Staehelin, T.; and Gordon, J. (1979): Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications, Proc Natl Acad Sci USA69:1404-1412.
33.
Zeichner-David, M.; Slavkin, H.C.; Lyaruu, I.M.; and Termine, J.D. (1983a): Biosynthesis and Secretion of Enamel Proteins During Hamster Tooth Development, Calcif Tissue Int35:366-371.
34.
Zeichner-David, M.; Macdougall, M.; and Slavkin, H.C. (1983b): Enamelin Gene Expression During Fetal and Neonatal Rabbit Tooth Organogenesis, Differentiation25:148-155.
