We undertook a microbiological study, in children, of dental plaque from sound surfaces or associated with rampant caries, both black-colored and unstained. Improved selective media allowed for the enumeration of bacteria belonging to specific genera or species present in plaque samples. A nearly similar bacterial distribution was found in both types of rampant caries. Aciduric flora, Streptococcus mutans, Veillonella, and Lactobacillus predominated in plaque over the lesions, whereas extracellular polysaccharide-producing streptococci other than S. mutans, as well as Actinomyces, were more abundant in plaque from sound surfaces. However, more lactobacilli and Actinomyces were recovered from pigmented lesions than from the unstained ones.
These findings suggest that the microbial flora associated with black-pigmented lesions did not strongly differ from that observed over unstained caries lesions.
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References
1.
Baird-Parker, A.C. (1957): Isolation of Leptotrichia buccalis and Fusobacterium Species from Oral Material, Nature (London ) 180:1056-1058.
2.
Beighton, D. and Colman, G. (1976): A Medium for Isolation and Enumeration of Oral Actinomycetaceae from Dental Plaque, J Dent Res55:875-878.
3.
Bowden, G.H.; Hardie, J.M.; McKee, A.S.; Marsh, P.D.; Fillery, E.D.; and Slack. G.L. (1976): The Microflora Associated with Developing Carious Lesions of the Distal Surfaces on the Upper First Premolars in 13-14 Year-old Children. In: Microbial Aspects of Dental Caries, H.M. Stiles, W.J. Loesche , and T.C. O'Brien, Eds., Vol. I, Washington, D.C.: Information Retrieval, Inc., pp. 223-241.
4.
Boyar, R.M. and Bowden, G.H. (1985): The Microflora Associated with the Progression of Incipient Carious Lesions in Teeth of Children Living in a Water-Fluoridated Area, Caries Res17 :532-542.
5.
Burt, B.A.; Loesche, W.J.; Eklund, S.A.; and Earnest, R.W. (1983): Stability of Streptococcus mutans and its Relationship to Caries in a Child Population over 2 Years, Caries Res17:532-542.
6.
Dreizen, S. and Spies, T. (1951): The Decalcification and Discoloration of Intact Noncarious Human Tooth Crowns by an Oral Strain of Lactobacillus acidophilus , Oral Surg4:388-392.
7.
Duchin, S. and van Houte, J. (1978): Relationship of Streptococcus mutans and Lactobacilli to Incipient Smooth Surface Dental Caries in Man, Arch Oral Biol23:779-786.
8.
Ellen, R.P. and Balcerzak-Raczkowski, I.B. (1975): Differential Medium for Detecting Dental Plaque Bacteria Resembling Actinomyces viscosus and Actinomyces naeslundii, J Clin Microbiol2:305-310.
9.
Englander, H.R. and Jordan, H.V. (1972): Relation between Streptococcus mutans and Smooth Surface Caries in the Deciduous Dentition, J Dent Res51:1505-1506.
10.
Fitzgerald, R.J. and Adams, B.O. (1975): Increased Selectivity of Mitis Salivarius Agar Containing Polymyxin, J Clin Microbiol1:239-240.
11.
Frank, R.M. and Champy, M. (1962): A Propos de la Melanodontie Infantile, Rev Stomatol63:709-727.
12.
Gibbons, R.J. and McDonald, J.B. (1960): Haemin and Vitamin K Compounds as Required Factors for the Cultivation of Certain Strains of Bacteroides melaninogenicus, J Bacteriol80:164-170.
13.
Gilmour, M.N. and Poole, A.E. (1970): Growth Stimulation of the Mixed Microbial Flora of Human Dental Plaques by Haemin, Arch Oral Biol15:1343-1353.
14.
Gold, O.G.; Jordan, H.V.; and van Houte, J. (1973): A Selective Medium for Streptococcus mutans, Arch Oral Biol18 :1357-1364.
15.
Holbrook, W.P.; Ogston, S.A.; and Ross, F.W. (1978): A Method for the Isolation of Bacteroides melaninogenicus from the Human Mouth, J Med Microbiol11:203-207.
16.
Klein, J.P.; Guinard, M.; and Frank, R.M. (1973): Etude de la Flore Buccale Aérobie chez des Enfants Polycaries et Indemnes de Caries, Rev Odont StomXX: 17-21.
17.
Loesche, W.J.; Rowan, J.; Stratton, L.H.; and Loos, P.J. (1975): Association of Streptococcus mutans with Human Dental Decay, Infect Immun11:1252-1260.
18.
Loesche, W.J. and Syed, S.A. (1973): The Predominant Cultivable Flora of Carious Plaque and Carious Dentin, Caries Res7 :201-216.
19.
Meiers, J.C.; Wirthlin, M.R.; and Shklair, I.L. (1982): A Microbiological Analysis of Human Early Carious and Non-carious Fissures, J Dent Res61 :460-464.
20.
Mikx, F.H.; van der Hoeven, J.S.; Plasschaert, A.J.M.; and Maltha, J.C. (1976): Establishment of Defined Microbial Ecosystems in Germ-free Rats. II/Disassociation of Osborne-Mendel Rats with Veillonella alcalescens and Several Oral Micro-organisms, Caries Res10:49-58.
21.
Milnes, A.R. and Bowden, G.H.W. (1985): The Microflora Associated with Developing Lesions of Nursing Caries. Caries Res19:289-297.
22.
Minah, G.E. and Loesche, W.J. (1977): Sucrose Metabolism in Resting-Cell Suspensions of Caries Associated and Non-Caries Associated Dental Plaque, Infect Immun17:43-54.
23.
Nakamura, T.; Suginaka, Y.; Obata, N.; Yamazaki, N.; and Takazoe, I. (1978): Growth Inhibition of Streptococcus mutans by the Black Pigment (Haematin) of Bacteroides melaninogenicus, Arch Oral Biol23:593-595.
24.
McNamara, T.F.; Friedman, B.K.; and Kleinberg, I. (1979): The Microbial Composition of Human Incisor Tooth Plaque, Arch Oral Biol24:91-95.
25.
Palmer, J.D. (1971): Dietary Habits at Bedtime in Relation to Dental Caries in Children, Br Dent J130:288-293.
26.
Ripa, L.W. (1978): Nursing Habits and Dental Decay in Infants: Nursing Bottle Caries, J Dent Child45:274-275.
27.
Rogers, A.H.; van der Hoeven, J.S.; and Mikx, F.H.M. (1978): Inhibition of Actinomyces viscosus by Bacteriocin-producing Strains of Streptococcus mutans in the Dental Plaque of Gnotobiotic Rats, Arch Oral Biol23:477-483.
28.
Rogosa, M.; Fitzgerald, R.J.; McIntosh, M.E.; and Beaman, A.J. (1958): Improved Medium for Selective Isolation of Veillonella , J Bacteriol76:455-456.
Siegel, S. (1956): Non-parametric Statistics for the Behavioral Sciences , New York: McGraw-Hill Book Co., Inc., pp. 116-127; pp. 184-194.
31.
Syed, S.A. and Loesche, W.J. (1972): Survival of Human Dental Plaque Flora in Various Transport Media, Appl Microbiol24:638-644.
32.
Thomson, L.A.; Little, W.A.; Bowen, W.H.; Sierra, L.I.; Aguirre, M.; and Gillespie, G. (1980): Prevalence of Streptococcus mutans Serotypes, Actinomyces and Other Bacteria in the Plaque of Children, J Dent Res59:1581-1589.
33.
van Houte, J.; Gibbs, G.; and Butera, C. (1982): Oral Flora of Children with Nursing Bottle Caries , J Dent Res61:382-385.
34.
White, G.E. (1976): Nutrition in the Practice of Pediatric Dentistry , Dent Clin North Am20:507-517.
35.
Winter, G.B.; Crule, D.; Mailer, G.P.; James, C.; and Gordon, P.H. (1971): The Prevalence of Dental Caries in PreSchool Children Aged 1 to 4 Years, Br Dent J130:434-436.
