Effects of Photoinitiators for the Visible-light Resin System on Hemolysis of Dog Erythrocytes and Lipid Peroxidation of Their Components

Abstract

The visible-light (VL) polymerizing resin system with photoinitiators (photosensitizer and reducing agent) is widely used in modern dentistry. In this system, polymerization is initiated by photochemical reactions between the photosensitizers (aromatic or aliphatic ketones) and reducing agents (tertiary amines) during VL irradiation.

In order to simulate the VL resin-induced toxicity in dental pulps, the present investigation was conducted to determine whether hemolysis of dog erythrocytes and lipid peroxidation of their components occur on exposure to a VL resin system containing aromatic (9-fluorenone, benzil) or aliphatic ketones (camphoroquinone) plus tertiary amine [2-(dimethylamino) ethyl methacrylate].

It was demonstrated that the hemolysis and lipid peroxidation with aromatic ketones were markedly higher than with those of aliphatic ketones, even when the latter were used at higher concentrations. It was clear that peroxidation-induced hemolysis occurred and, further, that surface-active complexes between photosensitizers and reducing agents, which are formed by irradiation, promote hemolytic activity due to their hydrophobic interactions. VL resin-induced responses to dental pulps are probably promoted due to the double effects of unpolymerized monomers and of both radicals and surface-active complexes formed during irradiation.

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References

Allen, S.N. (1982): Developments in Polymer Photechemistry- 3. NJ Applied Science Publishers, pp. 167-186.

Dart, E.C. and Nemcek, J. (1979): Japan. Patent 54-10986.

Desai, I.D. ; Sawant, P.L. ; and Tappel, A.L. (1964): Peroxidative and Radiation Damage to Isolated Lysosomes, Biochim Biophys Acta 86:277-285.

Fee, J.A. ; Bergamini, R. ; and Briggs, R.G. (1975): Observation on the Mechanism of the Oxygen/Dialuric Acid-induced Hemolysis of Vitamin E-Deficient Rat Red Cells and the Protective Roles of Catalase and Superoxide Dismutase, Arch Biochim Biophys 169:160-167.

Fujisawa, S. ; Imai, Y. ; Kojima, K. ; and Masuhara, E. (1978): Studies on Hemolytic Activity of Bisphenol A Diglycidyl Methacrylate (BIS-GMA), J Dent Res 57:98-102.

Fujisawa, S. ; Kadoma, Y. ; and Masuhara, E. (1982): Action of Drugs, Detergents, and Monomers on Liposomes , J Dent Res 61:1206-1210.

Gutteridge, J.M.C. (1981): Thiobarbituric Acid-reactivity Following Iron-dependent Free-radical Damage to Amino Acids and Carbohydrates, FEBS Lett 128:343-346.

Hunter, F.E. ; Gebicki, P.E. ; Hoffsten, P.E. ; Weinstein, J. ; and Scott, A. (1963): Swelling and Lysis of Rat Liver and Mitochondria Induced by Ferrous Ions, J Biol Chem 238:828-835.

Kunimoto, M. ; Inoue, K. ; and Nojima, S. (1981): Effect of Ferrous Ion and Ascorbate-induced Lipid Peroxidation on Liposomal Membranes, Biochim Biophys Acta 646:169-178.

10.

Lampert, F. and Loew, R.K. (1974): Physikalische und Biologische Prufung des Nuva-Gerates , Zahnärztl Welt Rundschau 83 :696-699.

11.

Lawrence, H. ; Dillingham, E. ; Malik, M. ; and Autian, J. (1971): Development of Standard Toxicity Testing for Dental Materials Products and Exploratory Dental Toxicology, Publication Board Report 205740.

12.

Lee, H.L. ; Orlowski, J.A. ; and Rogers, B.J. (1976): A Comparison of Ultraviolet-curing and Self-curing Polymers in Preventive, Restorative, and Orthodontic Dentistry, Int Dent J 26 :134-151.

13.

Leibowitz, M.E. and Johnson, M.C. (1971): Relation of Lipid Peroxidation to Loss of Cations Trapped in Liposomes, J Lipid Res 12:662-670.

14.

Mandal, T.K. and Chatterjee, S.N. (1980): Ultraviolet- and Sunlight-induced Lipid Peroxidation in Liposomal Membrane, Radiat Res 83:290-302.

15.

Pryor, W.A. and Stanley, J.R. (1975): A Suggested Mechanism for the Production of Malonaldehyde during the Autoxidation of Polyunsaturated Fatty Acids. Nonenzymatic Production of Prostaglandin Endoperoxides during Autoxidation, J Org Chem 40 :3615-3617.

16.

Robinson, J.D. (1965): Structural Changes in Microsomal Suspensions. III. Formation of Lipid Peroxides, Arch Biochem Biophys 112:170-179.

17.

Smolen, J.E. and Shohet, S.B. (1974): Permeability Changes Induced by Peroxidation in Liposomes Prepared from Human Erythrocyte Lipids, J Lipid Res 15:273-279.

18.

Terakado, M. ; Yamazaki, Y. ; Tsujimoto, Y. ; Kawa-Shima, T. ; Nagashima, K. ; Ogawa, J. ; Fujita, Y. ; Sugita, H. ; Sakai, T. ; and Furuyama, S. (1984): Lipid Peroxidation as a Possible Cause of Benzoyl Peroxide Toxicity in Rabbit Dental Pulp - A Microsomal Lipid Peroxidation in vitro, J Dent Res 63:901-905.

19.

Wills, E.D. and Wilkinson, A.E. (1967): The Effect of Irradiation on Sub-Cellular Particles - Destruction of Sulfhydryl Groups, Int J Radiat Biol 13:45-55.