Protease activity was measured in dental plaque collected from patients with or without supragingival calculus. Plaque specimens from the calculus group showed significantly greater protease activity in the presence of 0.05% sodium thioglycollate than did those from the non-calculus group. No significant difference between the two groups was observed in the protease activity without sodium thioglycollate. There was no significant correlation between the age of the patients and the protease activity.
Get full access to this article
View all access options for this article.
References
1.
Bennick, A.; Cannon, M.; and Madapallimattam, G. (1979): The Nature of the Hydroxyapatite-binding Site in Salivary Acidic Proline-rich Proteins, Biochem J183:115-126.
2.
Bohlen, P.; Stein, S.; Dairman, W.; and Udenfriend, S. (1973): Fluorometric Assay of Proteins in the Nanogram Range, Arch Biochem Biophys155:213-220.
3.
Driessens, F.C.M.; Borggreven, J.M.P.M.; Verbeeck, R.M.H.; Van Dijk, J.W.E., and Feagin, F.F. (1985): On the Physicochemistry of Plaque Calcification and the Phase Composition of Dental Calculus, J Periodont Res20:329-336.
4.
Esumi, H.; Sato, S.; Sugimura, T.; and Okasaki, N. (1978): Purification and Properties of an Acid Protease from Human Ascitic Fluid, Biochim Biophys Acta523:191-197.
5.
Frostell, G. and Söder, P.-6. (1970): The Proteolytic Activity of Plaque and its Relation to Soft Tissue Pathology, Int Dent J20:436-450.
6.
Grøn, P. and Hay, D.I. (1976): Inhibition of Calcium Phosphate Precipitation by Human Salivary Secretions, Arch Oral Biol21: 201-205.
7.
Hay, D.I.; Smith, D.J.; Schluckebier, S.K.; and Moreno, E.C. (1984): Relationship between Concentration of Human Salivary Statherin and Inhibition of Calcium Phosphate Precipitation in Stimulated Human Parotid Saliva, J Dent Res63:857-863.
8.
Ishikawa, I.; Cimasoni, G.; and Ahmad-Sadeh, C. (1972): Possible Role of Lysosomal Enzymes in the Pathogenesis of Periodontitis. A Study on Cathepsin D in Human Gingival Fluid, Arch Oral Biol17:111-117.
9.
Kunimitsu, D.K. and Yasunobu, K.T. (1970): Chymopapain B. In: Methods in Enzymology, Vol. XIX, Proteolytic Enzymes, G.E. Perlmann and L. Lorand, Eds., New York: Academic Press, pp. 244-252.
10.
Mandel, I.D. (1974): Biochemical Aspects of Calculus Formation. II. Comparative Studies of Saliva in Heavy and Light Calculus Formers, J Periodont Res9:211-221.
11.
Morishita, M.; Watanabe, T.; and Iwamoto, Y. (1981): Some Properties of the Protease in Salivary Sediment , J Hiroshima Univ Dent Soc13:230-234.
12.
Tokumoto, K.; Ohata, N.; Watanabe, T.; and Iwamoto, Y. (1982): The Origin of the Salivary Protease Correlated with Calculus Index, J Hiroshima Univ Dent Soc14:11-14.
13.
Watanabe, T.; Shinmoto, M.; Toda, K.; Morishita, M.; and Iwamoto, Y. (1980a): The Relationship Between Calculus Index and Protease Activity in Saliva, J Dent Res59:138.
14.
Watanabe, T.; Ohata, N.; Morishita, M.; and Iwamoto, Y. (1980b): The Correlationship Between the Protease Activity and the Epithelial Cells in the Saliva from the Patients with Gingivitis or Marginal Periodontitis, Jpn J Periodontol22:246-251.
15.
Watanabe, T.; Ohata, N.; Morishita, M.; and Iwamoto, Y. (1981): Correlation Between the Protease Activities and the Number of Epithelial Cells in Human Saliva, J Dent Res60 :1039-1044.
16.
Watanabe, T.; Toda, K.; Morishita, M.; and Iwamoto, Y. (1982a): Correlations Between Salivary Protease and Supragingival or Subgingival Calculus Index, J Dent Res61:1048-1051.
17.
Watanabe, T.; Toda, K.; Morishita, M.; Shinmoto, M.; and Iwamoto, Y. (1982b): Partial Correlations among the Indices Related to Periodontal Disease, Jpn J Periodontol24 :564-570.
