A Comparison of Some of the Permeability Characteristics of Intact and Tape-stripped Hamster Cheek Pouches in vitro

Abstract

Cheek pouches were removed from anesthetized hamsters and were either tape-stripped or left intact and then mounted in Ussing chambers, where they were bathed in Krebs-Ringer bicarbonate saline.

A potential difference could be measured across all pouches and was stable for over an hour across the unstripped pouches. The electrical resistance across the unstripped pouches was high (8960 Ω.cm²), whereas the resistance across the tape-stripped pouches was significantly lower (p<0.01), suggesting that these pouches are likely to be more permeable than the unstripped pouches.

Reflection coefficients of urea, ethanol, and glycerol were derived by means of streaming potentials. Values for intact pouches were all close to 1 and for tape-stripped pouches were 0.938, 0.822, and 0.509, respectively.

Permeability coefficients for urea and ethanol across intact and tape-stripped pouches were, respectively, 12.5 × 10^-9 cm/min; 27.6 x 10^-9 cm/min; 2325 × 10^-9 cmlmin ; and 28471 × 10-9 cmlmin.

The hamster cheek pouch has a high electrical resistance; it is an impermeable structure in which the keratin layer appears to provide a major barrier to the passage of water, ions, and certain solutes.

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References

Adams, D. (1974): Penetration of Water Through Human and Rabbit Oral Mucosa in vitro, Arch Oral Biol 19:865-872.

Addy, M. (1980): The Oral Mucosal Absorption and Tissue Distribution of Triamcinolone Acetonide in the Dog Studied by Autoradiography, Arch Oral Biol 25:809-817.

Bergman, S. ; Kane, D. ; Siegel, LA ; and Ciancio, S. (1969): In vitro and in situ Transfer of Local Anaesthetics Across the Oral Mucosa, Arch Oral Biol 14:35-43.

Claude, P. and Goodenough, D.A. (1973): Fracture Faces of Zonulae Occludentes from "Tight" and "Leaky" Epithelia, J Cell Biol 58:390-400.

Evans, T. ; Griffith, S.G. ; Kohn, P.G. ; McKeever, A. ; Sid-Dons, B.M. ; Simpson, J. ; Stobbs, M. ; Syme, G.B. ; and Wilson, E.L. (1979): Preliminary Study on Electrical Properties of Hamster Cheek Pouch, J Dent Res 58:1265, Abstr. No. 180.

Galey, W.R. ; Lonsdale, H.K. ; and Nachts, S. (1976): The in vitro Permeability of Skin and Buccal Mucosa to Selected Drugs and Tritiated Water, J Invest Dermatol 67:713-717.

Hingson, D.J. and Diamond, J.M. (1972): Comparison of Non-electrolyte Permeability Patterns in Several Epithelia, J Membrane Biol 10:93-135.

Kaaber, S. (1971a): Studies on the Permeability of Human Oral Mucosa. II. The Permeability of Dry Palatal Mucosa to Water, Sodium and Potassium, Acta Odontol Scand 29:663-681.

Kaaber, S. (1971b): Studies on the Permeability of Human Oral Mucosa. III. The Permeability of Dry Buccal Mucosa to Water, Sodium and Potassium, Acta Odontol Scand 29:683-698.

10.

Kaaber, S. (1973a): Studies on the Permeability of Human Oral Mucosa. IV. Regional Changes in Outflow of Water from Hydrated and Dehydrating Oral Mucosa, Acta Odontol Scand 31 :89-99.

11.

Kaaber, S. (1973b): Studies on the Permeability of Human Oral Mucosa. V. The Inflow of Water Through Dehydrating Oral Mucosa, Acta Odontol Scand 31:101-108.

12.

Pimlott, S.J. and Addy, M. (1983): Absorption of Prednisolone from Mouthwashes: The Effect of Concentration, Time and Oral Mucosal Site, J Dent Res 62:416, Abstr. No. 17.

13.

Scheuplein, R.J. and Blank, I.H. (1971): Permeability of the Skin, Physiol Rev 51:702-747.

14.

Siegel, I.A. ; Izutsu, K.T. ; and Burkhart, J. (1976): Transfer of Alcohols and Ureas Across the Oral Mucosa Measured using Streaming Potentials and Radioisotopes, J Pharmacol Sci 65 :129-131.

15.

Siegel, I.A. ; Izutsu, K.T. ; and Watson, E. (1981): Mechanisms of Non-electrolyte Penetration Across Dog and Rabbit Oral Mucosa in vitro, Arch Oral Biol 26:357-361.

16.

Shimono, M. and Clementi, F. (1976): Intercellular Junctions of Oral Epithelium. I. Studies with Freeze-Fracture and Tracing Methods of Normal Rat Keratinized Oral Epithelium, J Ultrastruct Res 56:121-136.

17.

Squier, C.A. and Rooney, L. (1976): The Permeability of Keratinized and Nonkeratinized Oral Epithelium to Lanthanum in vivo, J Ultrastruct Res 54:286-295.

18.

Wright, E.M. and Diamond, J.M. (1969): An Electrical Method of Measuring Non-electrolyte Permeability, Proc R Soc (B) 172 :203-225.