Sixty-two facultative Actinomyces strains isolated from dental plaque were tested for the production of bacteriocin-like inhibitory effects by a deferred antagonism method. When incubated anaerobically, all isolates produced identical inhibitory patterns against 15 indicator organisms, but under microaerophilic conditions, little inhibitory activity was observed. Activity was not evident after anaerobic incubation on a medium buffered by 0.5% (wlv) calcium carbonate. Gas-liquid chromatographic analyses of agar blocks removed from the inhibitory zones indicated that, compared with microaerophilic conditions, anaerobic incubation encouraged production of high concentrations of lactic and succinic acids, and the concomitant fall in the pH was probably responsible for the inhibitory effects.
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References
1.
Bowden, G.H.W.; Ellwood, D.C.; and Hamilton, I.R. (1979): Microbial Ecology of the Oral Cavity. In: Advances in Microbial Ecology, Vol. 3, M. Alexander, Ed., New York: Plenum Press, pp. 135-217.
2.
Buchanan, B.B. and Pine, L. (1967): Path of Glucose Breakdown and Cell Yields of a Facultative Anaerobe, Actinomyces naeslundii, J Gen Microbiol46:225-236.
3.
Dajani, A.S.; Tom, M.C.; and Law, D.J. (1976): Viridins, Bacteriocins of Alpha-hemolytic Streptococci: Isolation, Characterization, and Partial Purification, Antimicrob Agents Chemother9 :81-88.
4.
Donoghue, H.D. and Tyler, J.E. (1975): Antagonisms amongst Streptococci Isolated from the Human Oral Cavity, Arch Oral Biol20:381-387.
5.
Ellen, R.P. (1976): Establishment and Distribution of Actinomyces viscosus and Actinomyces naeslundii in the Human Oral Cavity, Infect Immun14:1119-1124.
6.
Franker, C.K.; Herbert, C.A.; and Ueda, S. (1977): Bacteriocin from Actinomyces odontolyticus with Temperature-dependent Killing Properties, Antimicrob Agents Chemother12 :410-417.
7.
Harper, D.S. and Loesche, W.J. (1984): Growth and Acid Tolerance of Human Dental Plaque Bacteria, Arch Oral Biol29 :843-848.
8.
Holdeman, L.V.; Cato, E.P.; and Moore, W.E.C. (Eds.) (1977): Anaerobe Laboratory Manual, 4th ed. Blacksburg, VA: Virginia Polytechnic Institute and State University Anaerobe Laboratory.
9.
Holmberg, K. and Hallander, H.O. (1972): Interference between Gram-positive Microorganisms in Dental Plaque, J Dent Res51:588-595.
10.
Jordan, H.V. and Hammond, B.F. (1972): Filamentous Bacteria Isolated from Human Root Surface Caries, Arch Oral Biol17:1333-1342.
11.
Kleinberg, I. (1982): In: Oral Microbiology, 4th ed., W.A. Nolte, Ed., St. Louis, MO: C.V. Mosby Co., pp. 605-624.
12.
Kornman, K.S. and Loesche, W.J. (1978): New Medium for Isolation of Actinomyces viscosus and Actinomyces naeslundii from Dental Plaque, J Clin Microbiol7:514-518.
13.
Rogers, A.H. (1975): Bacteriocin Types of Streptococcus mutans in Human Mouths, Arch Oral Biol20:853-858.
14.
Sandham, H.J. and Phillips, H.I. (1976): Relation between Acidogenic and Aciduric Properties of Microorganisms from Dental Plaque. In: Proceedings, Microbial Aspects of Dental Caries, H.M. Stiles , W.J. Loesche, and T.C. O'Brien, Eds., Sp. Supp. Microbiology Abstracts, Vol. II, New York: Information Retrieval, Inc., pp. 557-562.
15.
Slots, J.; Moenbo, D.; Langebaek, J.; and Frandsen, A. (1978): Microbiota of Gingivitis in Man, Scand J Dent Res86 :174-181.
16.
Socransky, S.S.; Manganiello, A.D.; Propas, D.; Oram, V.; and Van Houte, J. (1977): Bacteriological Studies of Developing Supragingival Dental Plaque, J Periodont Res12 :90-106.
17.
Syed, S.A. and Loesche, W.J. (1978): Bacteriology of Human Experimental Gingivitis: Effect of Plaque Age, Infect Immun21 :821-829.
18.
Tagg, J.R. and Bannister, L.V. (1979): "Fingerprinting" Betahaemolytic Streptococci by their Production of and Sensitivity to Bacteriocine-like Inhibitors, J Med Microbiol12:397-411.
19.
Tagg, J.R.; Dajani, A.S.; and Wannamaker, L.W. (1976): Bacteriocins of Gram-positive Bacteria, Bact Rev40:722-756.
20.
Turner, J.W. and Jordan, H.V. (1981): Bacteriocin-like Activity within the Genus Actinomyces , J Dent Res60:1000-1007.
21.
Van Houte, J. (1980): Bacterial Specificity in the Etiology of Dental Caries, Int Dent J30:305-326.
22.
Weerkamp, A.; Vogels, G.D.; and Skotnicki, M. (1977): Antagonistic Substances Produced by Streptococci from Human Dental Plaque and their Significance in Plaque Ecology, Caries Res11:245-256.
