Three lactate-dehydrogenase-deficient mutants of serotype c S. mutans were made by using, as parents, two serotype c strains that produced unusually large amounts of ethanol, acetic acid, and acetoin, and very little lactic acid, when grown in broth containing a limiting amount of glucose. The mutants, obtained with N-methyl-N'nitro-N-nitrosoguanidine, were stable during 12 weeks of daily subculture in broth. Crude cell-free extracts of the mutants had less than 1% of the LDH-specific activity of their parent strains. The serotype c mutants resembled serotype g mutants in having molar growth yields at least as high as those of their parents. However, in contrast to the g mutants, the c mutants produced cell crops (cell mass per ml medium) that were as high as those of their parent strains.
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References
1.
Bochner, E.R. and Savageau, M.A. (1977): Generalized Indicator Plate for Genetic, Metabolic, and Taxonomic Studies with Microorganisms, Appl Environ Microbiol33:434-444.
2.
Brown, A.T. and Wittenberger, C.L. (1972): Fructose-1,6-Diphosphate-Dependent Lactate Dehydrogenase from a Cariogenic Streptococcus: Purification and Regulatory Properties, J Bacteriol110:604-615.
3.
Cohen, L.H. and Noel, W.K. (1960): Glucose Catabolism of Rabbit Retina Before and After Development of Visual Function, J Neurochem5:253-276.
4.
Geddes, D.A. and Gilmour, M.N. (1970): The Control of Ghosting, a Major Source of Error in Gas-Liquid Chromatographic Determinations of C2 - Cs Acids, J Chrom Sci8:394-397.
5.
Gold, O.G.; Jordan, H.V.; and Van Houte, J. (1973): A Selective Medium for Streptococcus mutans, Arch Oral Biol20 :473-477.
6.
Hamada, S.; Masuda, N.; and Kotani, S. (1980): Isolation and Serotyping of Streptococcus mutans from Teeth and Feces in Children, J Clin Microbiol11:314-318.
7.
Hillman, J.D. (1978): Lactate Dehydrogenase-Deficient Mutants of Streptococcus mutans: Isolation and Preliminary Characterization, Infect Immun21:206-212.
8.
Ikeda, T.; Mukasa, T.; Yagi, S.; Ochiai, K.; Doi, Y.; Shiota, T.; and Sandham, H.J. (1975): The Characteristics of Streptococcus mutans PS-14 , Nihon Univ J Oral Sci1:202-207.
9.
Johnson, C.P.; Gross, S.M.; and Hillman, J.D. (1980): Cariogenic Potential in vitro in Man and in vivo in the Rat of Lactate Dehydrogenase Mutants of Streptococcus mutans, Arch Oral Biol25:707-713.
10.
Johnson, K.P. and Hillman, J.D. (1982): Competitive Properties of Lactate Dehydrogenase Mutants of the Oral Bacterium Streptococcus mutans in the Rat, Arch Oral Biol27:513-516.
11.
Lederberg, J. (1948): Detection of Fermentative Variants with Tetrazolium , J Bacteriol56:695.
12.
Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; and Ran-Dall, R.J. (1951): Protein Measurement with the Folin Phenol Reagent , J Biol Chem193:265-275.
13.
McCabe, R.; Keyes, P.; and Howell, A., Jr. (1967): An in vitro Method for Assessing the Plaque-Forming Abilities of Oral Bacteria, Arch Oral Biol12:1653-1656.
14.
Morris, D.L. (1948): Quantitative Determination of Carbohydrates with Dreywood's Anthrone Reagent, Science107:254-255.
15.
Salem, H.H.; Sandham, H.J.; and Chan, K.H. (1985): Lactate Dehydrogenase-deficient Mutants of Serotype g Streptococcus mutans, J Dent Res64:1191-1194.
16.
Shklair, I.L. and Keene, H.J. (1974): A Biochemical Scheme for the Separation of the Five Varieties of Streptococcus mutans, Arch Oral Biol19:1079-1081.
17.
Takehashi, S.; Abbe, K.; and Yamada, T. (1982): Purification of Pyruvate Formate-Lyase from Streptococcus mutans and Its Regulatory Properties, J Bacteriol149:1034-1040.
18.
Thomson, L.A.; Little, W.A.; Bowen, W.H.; and Ha-Geage, G.J. (1980): Prevalence of Streptococcus mutans Serotypes, Actinomyces and Other Bacteria in the Plaque of Children, J Dent Res59:1581-1589.
19.
Van Der Hoeven, J.S. (1976): Carbohydrate Metabolism of Streptococcus mutans in Dental Plaque in Gnotobiotic Rats, Arch Oral Biol21 :431-433.
20.
Yamada, T. and Carlsson, J. (1976): The Role of Pyruvate Formate-Lyase in Glucose Metabolism of Streptococcus mutans. In: Microbial Aspects of Dental Caries, Vol. 3, H.M. Stiles, W.J. Loesche, and T.C. O'Brien, Eds., Washington, D.C.: Information Retrieval, Inc., pp. 809-819.
21.
Yamada, T.; Endo, K.; and Araya, S. (1976): A Fructose-1,6-Diphosphate-Dependent L-Lactate Dehydrogenase in a Strain of Streptococcus mutans, Arch Oral Biol21:233-236.
22.
Yamada, T.; Takahashi-Abbe, S.; and Abbe, K. (1985): Effects of Oxygen on Pyruvate Formate-Lyase in situ and Sugar Metabolism of Streptococcus mutans and Streptococcus sanguis , Infect Immun47:129-134.
23.
Yaphe, B.; Johnson, K.; Shuh, V.; and Hillman, J. (1982): Replacement Therapy of Dental Caries in Irus Monkeys , J Dent Res61:252.
