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Abstract

The effects of three anti-oxidants — tertiary butylhydroquinone (TBHQ), butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT) - on both growth and lactic acid production by eight cariogenic strains of Streptococcus mutans were investigated. Synergistic inhibitory effects of potassium sorbate on lactic acid production were also determined. All three anti-oxidants are phenolic derivatives and are commonly used in food systems due to their excellent "carry-through" properties during processing. Growth inhibition was determined by turbidity measurements at 600 nm. Lactic acid was assayed by gas chromatography, and bacterial DNA was assayed by the diphenylamine reaction. There were reduced growth levels of S. mutans due to the anti-oxidants and potassium sorbate for at least 12 hr, with TBHQ and BHA still inhibiting growth at 24 hr. Nearly all concentrations of anti-oxidants and potassium sorbate reduced lactic acid production by S. mutans, but only TBHQ significantly inhibited lactic acid production when the amount of acid per μg DNA was calculated. A synergistic reduction of lactic acid production by S. mutans does occur in most combinations of potassium sorbate with anti-oxidants.

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References

Anaerobe Laboratory Manual, 4th ed., Staff of V.P.I. Anaerobe Laboratory. The Virginia Polytechnic Institute and State University Anaerobe Laboratory, P.O. Box 49, Blacksburg, VA 24060.

Davidson, P.M. ; Brekke, C.J. ; and Branen, A.L. (1981): Anti-microbial Activity of Butylated Hydroxyanisole, Tertiary Butylhydroquinone, and Potassium Sorbate in Combination, J Food Sci 46:314.

Dische, Z.I. (1957): Color Reactions of Nucleic Acids and their Constituents Based on Reactions of their Sugars. In: Enzymology III , G. Ashwell , Ed., New York: Academic Press.

Glass, R.L., Ed. (1982): The First International Conference on the Declining Prevalence of Dental Caries , J Dent Res 61(Sp Iss) :1301-1388.

Klindworth, K.J. ; Davidson, P.M. ; Brekke, C.J. ; and Branen, A.L. (1979): Inhibition of Clostridium perfringens by Butylated Hydroxyanisole, J Food Sci 44:564-567.

Lahellec, C. ; Fung, D.Y.C. ; and Cunningham, F.E. (1981): Growth Effect of Sorbate and Selected Antioxidants on Toxigenic Strains of Staphylococcus aureus, J Food Protect 44:531-534.

Osborne, M. ; Lamberts, B.L. ; Meyer, T.S. ; and Roush, A.H. (1976): Acrylamide Gel Electrophoretic Studies of Extracellular Sucrose-Metabolizing Enzymes of Streptococcus mutans, J Dent Res 55:77-83.

Raccach, M. and Henningsen, E.C. (1981): Antibacterial Effect of Tertiary Butylhydroquinone Against Two Genera of Gram Positive Cocci, J Food Sci 47:106-110.

Robach, M.C. and Stateler, C.L. (1980): Inhibition of Staphylococcus aureus by Potassium Sorbate in Combination with Sodium Chloride, Tertiary Butylhydroquinone, Butylated Hydroxyanisole or Ethylenediamine Tetraacetic Acid, J Food Protect 43:208-211.

10.

Winer, B.J. (1971): Statistical Principles in Experimental Design , 2nd ed. New York: McGraw-Hill Book Co., pp. 149-219.

Effect of Anti-oxidants on Growth and Lactic Acid Production by Streptococcus mutans

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