The acid production rate of dental plaque, formed by different streptococci in gnotobiotic rats fed a high sucrose diet or starved for seven or eight days, was determined in vitro. Starvation had no appreciable effect on acid production. This supports the idea that the small pH-lowering ability of plaque of caries-free or stomach tube-fed people in whom exposure of the teeth to fermentable carbohydrate is relatively low or negligible results from a change in the bacterial population rather than a change in the rate of acid production by an unaltered flora.
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