Twenty-eight fresh and seven type strains of Lactobacillus species were tested for their ability to grow and metabolize glucose in the presence of fluoride. While there was variation between the test organisms, freshly isolated strains were generally more fluoride-tolerant than were the type strains, and a group of seven strains would grow well at pH 4.5 with 5.3 mm NaF. Glucose metabolism by representative strains of this latter group required average fluoride concentrations of 204, 64, and 9.3 mM to inhibit glycolysis completely at pH 7.0, 6.0, and 5.0, respectively. These values are ten times higher than those required to inhibit glucose metabolism by Streptococcus mutans.
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References
1.
Borei, H. (1945): Inhibiting Cellular Oxidation by Fluoride, Ark Kemi Miner Geol20A:1-215.
2.
Bowden, G.H.; Hardie, J.M.; McKee, A.S.; Marsh, P.D.; Fillery, E.D.; and Slack, G.L. (1976): The Microflora Associated with Developing Carious Lesions on the Distal Surfaces on the Upper First Premolars in 13-14 Year Old Children. In: Microbial Aspects of Dental Caries, Vol. 1. Microbiol. Abst. Spec. Suppl., Stiles, H.M., Loesche, W.J., and O'Brien, T.C., Eds., Washington, D.C .: Information Retrieval, Inc., pp. 223-241.
3.
Bowden, G.H.; Odlum, 0.; Nolette, N.P.; and Hamilton, I.R. (1982): Microbial Populations Growing in the Presence of Fluoride at Low pH Isolated from Dental Plaque of Children Living in an Area with Fluoridated Water, Infect Immun36: 247-254.
4.
Boyar, R.M. and Bowden, G.H. (1983): The Association of Lactobacillus with Progressing Incipient Carious Lesions in Children from a Water Fluoridated Area, J Dent Res62:176.
5.
Boyar, R.M. and Bowden, G.H. (1985): The Microflora Associated with the Progression of Incipient Carious Lesions in Teeth of Children Living in a Water Fluoridated Area, Caries Res, In press.
6.
Buchanan, R.E. and Gibbons, N.E., Eds. (1974): Bergey's Manual of Determinative Bacteriology. Baltimore: Williams and Wilkins Co., pp. 576-592.
7.
De Man, J.C.; Rogosa, M.; and Sharpe, M.E. (1960): A Medium for the Cultivation of Lactobacilli, J Appl Bacteriol23: 130.
8.
Edwardsson, S. (1974): Bacteriological Studies on Deep Areas of Carious Dentine, Odontol Revy25 (Suppl. 32):1-43.
9.
Eisenberg, A.D.; Bender, G.R.; and Marquis, R.E. (1980): Reduction in the Aciduric Properties of the Oral Bacterium Streptococcus mutans GS-5 by Fluoride, Arch Oral Biol25 :133-135.
10.
Fitzgerald, R.J.; Adams, B.O.; Fitzgerald, D.B.; and Knox, K.W. (1981): Cariogenicity of Human Plaque Lactobacilli in Gnotobiotic Rats, J Dent Res60:919-926.
11.
Hamilton, I.R. (1977): Effects of Fluoride on Enzymatic Regulation of Bacterial Carbohydrate Metabolism, Caries Res11 (Suppl. 1):262-291.
12.
Hamilton, I.R. and Ellwood, D.C. (1978): Effects of Fluoride on Carbohydrate Metabolism by Washed Cells of Streptococcus mutans Grown at Various pH Values in a Chemostat , Infect Immun19:434-442.
13.
Hamilton, I.R. and Bowden, G.H. (1982): Response of Freshly Isolated Strains of Streptococcus mutans and Streptococcus mitior to Change in pH in the Presence and Absence of Fluoride during Growth in Continuous Culture, Infect Immun36 :255-262.
14.
Hardie, J.M.; Thompson, P.L.; South, R.J.; Marsh, P.D.; Bowden, G.H.; McKee, A.S.; Fillery, E.D.; and Slack, G.L. (1977): A Longitudinal Epidemiological Study on Dental Plaque and the Development of Dental Caries, J Dent Res56 (C):90-98.
15.
Ikeda, T.; Sandham, H.J.; and Bradley, E.L. (1973): Changes in Streptococcus mutans and Lactobacilli in Relation to the Initiation of Dental Caries in Negro Children, Arch Oral Biol18:555-566.
16.
Kanapka, J.A. and Hamilton, I.R. (1977): Fluoride Inhibition of Enolase Activity in vivo and its Relationship to the Inhibition of Glucose-6-Phosphate Formation in Streptococcus salivarius, Arch Biochem Biophys146:167-174.
Loesche, W.J. and Straffon, L.H. (1979): Longitudinal Investigation of the Role of Streptococcus mutans in Human Tissue Decay, Infect Immun26:498-507.
19.
Maltz, M. and Emilson, C.G. (1982): Susceptibility of Oral Bacteria to Various Fluoride Salts, J Dent Res61:786-790.
20.
Milnes, A.R. and Bowden, G.H. (1985): The Microflora Associated with Developing Lesions of Nursing Caries, Caries Res, In press.
21.
Milnes, A.R.; Lebtag, I.H.; and Bowden, G.H. (1984): The Sensitivity of Recent Isolates of Lactobacillus Species to Fluoride at Different pH Levels, J Dent Res63:229.
22.
Okuda, K. and Frostell, G. (1982): The Effect of Fluoride on the Acid Production of Streptococcus mutans and other Oral Streptococci, Swed Dent J6:29-36.
23.
Whitford, G.M.; Schuster, G.S.; Pashley, D.H.; and Venkateswarlu, P. (1977): Fluoride Uptake by Streptococcus mutans 6715, Infect Immun24:680-687.
