Oligosaccharide Structures of the Low-molecular-weight Salivary Mucin from a Normal Individual and one with Cystic Fibrosis

Abstract

Studies were begun to compare the oligosaccharide structures of the low-molecular-weight mucin purified from the submandibular-sublingual saliva of a normal individual with that from one with cystic fibrosis. Following alkaline/borotritide cleavage, neutral and sialic acid-containing chains were purified by a combination of gel filtration, paper chromatography, and high-voltage paper electrophoresis. Oligosaccharides ranged in size from a disaccharide to a heptasaccharide. Approximately 80% of the oligosaccharides were GalB1, 3 GalNAc; Fucα1,2GalB1,3GalNAc; and NeuAcα2,3GalB1, 3GalNAc. The other structures were Fucα1,2GalB1,4(Fucα1,3)GlcNAcB1,6 (GalB1,3)GalNAc or GalB1,4(Fucα1,3)GlcNAcB1,6(Fucα1,2GalB1, 3)GalNAc; GalB1,4(Fucα1,3)GlcNAcB1,6(NeuAcα2,3GalB1,3)GalNAc; and Fucα1,2GalB1,4(Fucα1,3)GlcNAcB1,6 (NeuAcα2,3Gal-B1,3)GalNAc. There were no apparent qualitative differences in the neutral and sialic acid-containing units recovered from the normal and cystic fibrosis samples.

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References

Boat, T.F. and Cheng, P.W. (1976): Mucous Glycoproteins. In: Cystic Fibrosis: Projections into the Future, Mangos, J.A. and Talins, R.C. , Eds. New York: Stratton Intercont. Med. Book Corp., pp. 165-177.

Boat, T.F. ; Cheng, P.W. ; Iyer, R.N. ; Carlson, D.M. ; and Polony, I. (1976): Mucous Glycoproteins of Non-purulent Tracheobronchial Secretions and Sputum of Patients with Bronchitis and Cystic Fibrosis, Arch Biochem Biophys 177: 95-104.

Davis, P.B. and Di-Sant'Agnese, P.A. (1984): Diagnosis and Treatment of Cystic Fibrosis. An Update, Chest 85:802-809.

Frates, R.C., Jr. ; Kaizu, T.T. ; and Last, J.A. (1983): Mucus Glycoproteins Secreted by Respiratory Epithelial Tissue from Cystic Fibrosis Patients, Pediatr Res 17:30-34.

Hakomori, S. (1964): A Rapid Permethylation of Glycolipid and Polysaccharide Catalyzed by Methylsulfinyl Carbanion in Dimethyl Sulfoxide, J Biochem (Tokyo) 55:205-208.

Kessler, M.J. ; Reddy, M.S. ; Shah, R.H. ; and Bahl, O.P. (1979): Structures of N-glycosidic Carbohydrate Units of Human Chorionic Gonadotropin, J Biol Chem 254:7901-7908.

Lamblin, G. ; Boersma, A. ; Klein, A. ; Roussel, P. ; Van Halbeek, H. ; and Vliegenthart, F.G. (1984): Primary Structure Determination of Five Sialylated Oligosaccharides Derived From Bronchial Mucus Glycoproteins of Patients Suffering from Cystic Fibrosis, J Biol Chem 259:9051-9058.

Prakobphol, A. ; Levine, M.J. ; Tabak, L.A. ; and Reddy, M.S. (1982): Purification of a Low Molecular Weight Mucin-Type Glycoprotein from Human Submandibular-Sublingual Saliva, Carbohydr Res 108:111-122.

Reddy, M.S. ; Levine, M.J. ; and Tabak, L.A. (1982): Structure of the Carbohydrate Chains of the Proline-Rich Glycoprotein from Human Parotid Saliva, Biochem Biophys Res Commun 104:882-888.

10.

Roussel, P. ; Lamblin, G. ; Degand, P. ; Walker-Nasir, E. ; and Jeanloz, R.M. (1976): Heterogeneity of the Carbohydrate Chains of Sulfated Bronchial Glycoproteins Isolated from a Patient Suffering from Cystic Fibrosis , J Biol Chem 250 :2114-2122.

11.

Spiro, R.G. and Bhoyroo, V.D. (1974): Structure of the O-glycosidically Linked Carbohydrate Units of Fetuin, J Biol Chem 249:5704-5717.

12.

Stellner, K. ; Saito, K. ; and Hakomori, S. (1973) :Determination of Amino Sugar Linkages in Glycolipids by Methylation, Arch Biochem Biophys 155:464-472.

13.

Tabak, L.A. ; Reddy, M.S. ; and Levine, M.J. (1982): Characterization of a Pentasaccharide in Salivary Mucin from the Stumptail Monkey Macaca arctoides, Arch Oral Biol 27:297-303.

14.

Trevelyan, W.E. ; Proctor, D.P. ; and Harrison, J.S. (1950): Detection of Sugars on Paper Chromatograms, Nature 166:444-445.

15.

Van Halbeek, H. ; Dorland, L. ; Vliegenthart, J.F.G. ; Hull, W.E. ; Lamblin, G. ; Lhermitte, M. ; Boersma, A. ; and Roussel, P. (1982): Primary Structure Determination of Fourteen Neutral Oligosaccharides Derived from Bronchial-Mucus Glycoproteins of Patients Suffering from Cystic Fibrosis, Employing 500-MHz 1H-NMR Spectroscopy, Eur J Biochem 127:7-20.

16.

Wesley, A.W. ; Forstner, J.F. ; Qureshi, R. ; Mantle, M. ; and Forstner, G.G. (1983): Human Intestinal Mucin in Cystic Fibrosis, Pediatr Res 17:65-69.

17.

Yurewicz, E.C. and Moghissi, K.S. (1981): Purification of Human Midcycle Cervical Mucin and Characterization of its Oligosaccharides with Respect to Size, Composition, and Micro-heterogeneity, J Biol Chem 256:11895-11904.