Various sucrose derivatives and a sucrose analogue were enzymatically synthesized on the assumption that structural similarities of the sugars to sucrose would endow them with an ability to inhibit the cariogenicity of sucrose. The effect of these sugars on sucrose-dependent synthesis and adherence of insoluble glucan and on acid fermentation of sucrose by Streptococcus mutans was examined in vitro. Although none of them inhibited the acid fermentation, several sugars not only inhibited the synthesis and adherence of insoluble glucan, but were themselves only slightly fermented as well.
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References
1.
Avigad, G. (1957): Enzymatic Synthesis and Characterization of a New Trisaccharide, α-Lactosyl-β-fructofuranoside, J Biol Chem229:121-129.
2.
Avigad, G.; Feingold, D.S.; and Hestrin, S. (1956): An Enzymic Synthesis of a Sucrose Analogue: a-D-Xylopyranosyl-β-D-fructofuranoside , Biochim Biophys Acta20:129-134.
3.
Barker, S.A.; Bourne, E.J.; and Theander, O. (1957): Studies of Aspergillus niger. Part V. The Enzymic Synthesis of a New Trisaccharide, J Chem Soc11:2064-2067.
4.
Dubois, M.; Gilles, K.A.; Hamilton, J.K.; Rebers, P.A.; and Smith, F. (1956): Colorimetric Method for Determination of Sugars and Related Substances, Analyt Chem28:350-356.
5.
Fitzgerald, R.J.: The Microbial Ecology of Plaque in Relation to Dental Caries. In: Microbial Aspects of Dental Caries, H.M. Stiles, W.J. Loesche, and T.C. O'Brien, Eds., Washington D.C .: Information Retrieval, Inc., 1976, pp. 840-858.
6.
French, D.; Levine, M.L.; Norberg, E.; Nordin, P.; Pazur, J.H.; and Wild, G.M. (1954): Studies on the Schardinger Dextrins. VII. Co-substrate Specificity in Coupling Reactions of Macerans Amylase, J Am Chem Soc76:2387-2390.
7.
Frostell, G.; Blomlof, L.; Blomqvist, T.; Dahl, G.M.; Edward, S.; Fjellstrom, A.; Henriksson, C.O.; Nord, C.E.; and Nordenwall, K.J. (1974): Substitution of Sucrose by Lycasin® in Candy: "The Roslagen Study", Acta Odontol Scand32:235-254.
8.
Frostell, G.; Keyes, P.H.; and Larson, R.H. (1967): The Effect of Various Sugars and Sugar Substitutes on Dental Caries in Hamsters and Rats, J Nutrit93:65-76.
9.
Gibbons, R.J. and Van Houte, J. (1975): Dental Caries, Ann Rev Med26:121-136.
10.
Green, R.M. and Hartles, R.L. (1969): The Effect of Diets Containing Different Mono- and Disaccharides on the Incidence of Dental Caries in the Albino Rat, Arch Oral Biol14:235-241.
11.
Grenby, T.H. (1963): The Effect of Some Carbohydrates on Experimental Dental Caries in the Rat, Arch Oral Biol8:27-30.
12.
Guggenheim, B., Ed.: Health and Sugar Substitutes: Proceedings of the ERGOB Conference on Sugar Substitutes, Basel: S. Karger AG, 1979.
13.
Guggenheim, B.; Konig, K.G.; Herzog, E.; and Muhle-Mann, H.R. (1966): The Cariogenicity of Different Dietary Carbohydrates Tested on Rats in Relative Gnotobiosis with a Streptococcus Producing Extracellular Polysaccharide, Helv Odont Acta10:101-113.
14.
Huxley, H.G. (1974): The Effect of the Dietary Carbohydrate upon the Colonization of Plaque by Streptococcus mutans in Rats, Arch Oral Biol19:941-946.
15.
Igarashi, K.; Kamiyama, K.; and Yamada, T. (1981): Measurement of pH in Human Dental Plaque in vivo with an Ion-sensitive Transistor Electrode, Arch Oral Biol26 :203-207.
16.
Ikeda, T.; Shiota, T.; McGhee, J.R.; Otake, S.; Michalek, S.M.; Ochiai, K.; Hirasawa, S.; and Sugimoto, K. (1978): Virulence of Streptococcus mutans. Comparison of the Effects of a Coupling Sugar and Sucrose on Certain Metabolic Activities and Cariogenicity, Infect Immun19:477-480.
17.
Kitahata, S.; Tsuyama, N.; and Okada, S. (1974): Purification and Some Properties of Cyclodextrin Glycosyltransferase from a Strain of Bacillus Species, Agric Biol Chem38:387-393.
18.
Koulourides, T.; Bodden, R.; Keller, S.; Manson-Hing, L.; Lastra, J.; and Housch, T. (1976): Cariogenicity of Nine Sugars Tested with an Intraoral Device in Man, Caries Res10: 427-441.
19.
Krasse, B. (1965): The Effect of Caries-inducing Streptococci in Hamsters Fed Diets with Sucrose or Glucose, Arch Oral Biol10: 223-226.
20.
Mukasa, H. and Slade, H.D. (1973): Mechanism of Adherence of Streptococcus mutans to Smooth Surfaces. I. Role of Insoluble Dextran-levan Synthetase Enzymes and Cell Wall Polysaccharide Antigen in Plaque Formation, Infect Immun8:555-562.
21.
Newbrun, E. (1973): Sugar, Sugar Substitutes and Noncaloric Sweetening Agents, Int Dent J23: 328-345.
22.
Nisizawa, T.; Imai, S.; Akada, H; Hinoide, M.; and Araya, S. (1976): Extracellular Glucans Produced by Oral Streptococci, Arch Oral Biol21:207-213.
23.
Pazur, J.H. (1952): Transfructosidation Reactions of an Enzyme of Aspergillus oryzae, J Biol Chem199:217-225.
24.
Scheinin, A. and Makinen, K.K., Eds. (1975): Turku Sugar Studies I-XXI , Acta Odontol Scand33 (Suppl 70):1-351. Shaw, J.H.; Krumins , I.; and Gibbons, R.J. (1967): Comparison of Sucrose, Lactose, Maltose and Glucose in the Causation of Experimental Oral Disease , Arch Oral Biol12:755-768.
25.
Sherp, H.W. (1971): Dental Caries: Prospects for Prevention, Science173:1199-1205.
26.
Stephan, R.M. and Hemmens, E.S. (1947): Studies of Changes in pH Produced by Pure Culture of Oral Microorganisms, J Dent Res26:15-41.
27.
Tanaka, T.; 01, S.; Iizuka, M.; and Yamamoto, T. (1978): Levansucrase of Bacillus subtilis, Agric Biol Chem42:323-326.
28.
Yamada, T.; Igarashi, K.; and Mitsutomi, M. (1980): Evaluation of Cariogenicity of Glucosylsucrose by a New Method to Measure pH under Human Dental Plaque in situ, J Dent Res59 (DII):2157-2162.
