Fluoride (5 mg/l) consistently depressed the accumulation of intracellular iodophilic polysaccharides (IPS) in Streptococcus mutans strains BHT, FA-1, and GS-5 by over 90% and in Actinomyces viscosus strain RC-45 by over 50%. There was little further reduction in IPS content when fluoride was increased from 5 to 100 mg/l. Lithium (0 to 1 mg/l) neither enhanced nor inhibited IPS accumulation, nor did it modify the inhibitory effects of fluoride in three of the four strains tested. Strontium (0 to 100 mg/l) did not alter IPS accumulation in S. mutans GS-5 but decreased accumulation (<10%) in S. mutans FA-1 and BHT and significantly enhanced IPS accumulation in A. viscosus RC-45. Analysis of variance indicated no statistically significant interactions between fluoride and strontium, fluoride and lithium, or strontium and lithium.
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References
1.
Berman, K.S. and Gibbons, R.J.: Iodophilic Polysaccharide Synthesis by Human and Rodent Oral Bacteria , Arch Oral Biol11:533-542,1966.
2.
Birkhed, D. and Tanzer, J.M.: Glycogen Synthesis Pathway in Streptococcus mutans Strain NTCC10449S and Its Glycogen Synthesis-Defective Mutant 805, Arch Oral Biol24:67-73, 1979.
3.
Brown, W.E. and König, K.G.: Cariostatic Mechanisms of Fluorides, Caries Res11(Suppl 1):1-327, 1977.
4.
Curzon, M.E.J.: Combined Effect of Trace Elements and Fluorine on Caries: Changes Over 10 Years in NW Ohio (USA), J Dent Res62:96-99, 1983.
5.
Curzon, M.E.J. and Crocker, D.C.: Relationships of Trace Elements in Human Tooth Enamel to Dental Caries , Arch Oral Biol23:647-653, 1978.
6.
Curzon, M.E.J. and Losee, F.L.: Strontium Content of Enamel and Dental Caries, Caries Res11:321-326, 1977.
7.
Curzon, M.E.J. and Losee, F.L.: Trace Element Composition of Whole Human Enamel and Dental Caries. Part II . Western U.S.A., JADA96:819-822, 1978.
8.
DE Stoppelaar, J.D.; Van Houte, J.; and Backer-Dirks, O. : The Effect of Carbohydrate Restriction on the Presence of Streptococcus mutans, Streptococcus sanguis and Iodophilic Polysaccharide-Producing Bacteria in Human Dental Plaque, Caries Res4:114-123, 1970.
9.
Dipersio, J.R.; Mattingly, S.J.; Higgins, M.C.; and Shock-Man, G.D.: Measurement of Intracellular Iodophilic Polysaccharide in Two Cariogenic Strains of Streptococcus mutans by Cytochemical and Chemical Methods, Infect Immun10 :597-604,1974.
10.
Eisenberg, A.D.: Lithium. In: Trace Elements and Dental Disease. M.E.J. Curzon and T.W. Cutress, Eds., Boston, MA: John Wright-PSG, Inc., 1983, pp. 311-323.
Gallagher, I.H.C. and Cutress, T.W.: The Effect of Trace Elements on the Growth and Fermentation by Oral Streptococci and Actinomyces, Arch Oral Biol22:555-562, 1977.
13.
Gibbons, R.J. and Socransky, S.S.: Intracellular Polysaccharide Storage by Organisms in Dental Plaque , Arch Oral Biol7:73-80, 1962.
14.
Hamilton, I.R.: Studies with Fluoride-Sensitive and Fluoride-Resistant Strains of Streptococcus salivarius. I. Inhibition of Both Intracellular Polyglucose Synthesis and Degradation by Fluoride, Can J Microbiol15:1013-1019, 1969.
15.
Herbison, R.J. and Handelman, S.L.: Effect of Trace Elements on Dissolution of Hydroxyapatite by Cariogenic Streptococci , J Dent Res54:1107-1114, 1975 .
16.
Kanapka, J.A. and Hamilton, I.R.: Fluoride Inhibition of Enolase Activity in vivo and Its Relationship to the Inhibition of Glucose 6-P Formation in Streptococcus salivarius, Arch Biochem Biophys146:167-174, 1971.
17.
Kanapka, J.A.; Khandelwal, R.L.; and Hamilton, I.R.: Fluoride Inhibition of Glucose-6-P Formation in Streptococcus salivarius. Relation to Glycogen Synthesis and Degradation, Arch Oral Biol144:596-602, 1971.
18.
Kilian, M.; Thylstrup, A.; and Fejerskov, O.: Predominant Plaque Flora of Tanzanian Children Exposed to High and Low Water Fluoride Concentrations, Caries Res13:330-343, 1979.
19.
Loesche, W.J. and Henry, C.A.: Intracellular Microbial Polysaccharide Production and Dental Caries in a Guatemalan Indian Village, Arch Oral Biol12:189-194, 1967.
20.
Losee, F.L. and Bibby, B.G.: Caries Inhibition by Trace Elements Other Than Fluorine, NY Dent J36:15-19, 1970.
21.
Mitchell, P.: Keilin's Respiratory Chain Concept and Its Chemiosmotic Consequences , Science206:1148-1159, 1979 .
22.
Niiya, S.; Moriyama, Y.; and Futal, M.: Cation Coupling to Melibiose Transport in Salmonella, J Bacteriol144:192-199, 1980.
23.
Olson, B.; McDonald, J.; and Stookey, G.: The Effect of Strontium and Fluoride Upon in vitro Plaque and Rat Caries, J Dent Res57:903, 1978.
24.
Olson, B.; McDonald, J.; and Stookey, G.: The Effect of Lithium and Fluoride Upon in vitro Plaque and Bacterial Growth , J Dent Res58:1428, 1979.
25.
Preiss, J. and Greenberg, E.: Biosynthesis of Bacterial Glycogen. III. The Adenosine Diphosphate Glucose: a-4-Glucosyl Transferase of Escherichia coli B, Biochemistry4:2328-2334, 1965.
26.
Preiss, J. and Greenberg, E.: Biosynthesis of Bacterial Glycogen : Activator Specificity of the Adenosine Diphosphate Glucose Pyrophosphorylase from the Genus Rhodospirillum , J Bacteriol147:711-719, 1981.
27.
Rodriguez-Navarro, A.; Sancho, E.D.; and Perez-Loveres, C. : Energy Source for Lithium Efflux in Yeast , Biochem Biophys Acta640:352-358, 1981.
28.
Schamschula, R.G.; Adkins, B.L.; Barmes, D.E.; Charlton, G.; and Davey, B.G.: Caries Experience and the Mineral Content of Plaque in a Primitive Population in New Guinea, J Dent Res56(Spec Issue C):62, 1977.
29.
Schamschula, R.G.; Bunzel, M.; Agus, H.M.; Adkins, B.L.; Barmes, D.E.; and Charlton, G.: Plaque Minerals and Caries Experience: Associations and Interrelationships , J Dent Res57:427-432, 1978.
30.
Tanzer, J.M.; Freedman, M.L.; Woodiel, F.N.; Eifert, R.L.; and Rinehimer, L.A.: Association of S. mutans Virulence With Synthesis of Intracellular Polysaccharide . In: Microbial Aspects of Dental Caries, Vol. 3, H.M. Stiles, W.J. Loesche , and T.C. O'Brien, Eds., Washington, DC: Information Retrieval, Inc., 1977, pp. 597-616.
31.
Treasure, P.: Effects of Fluoride, Lithium, and Strontium on Extracellular Polysaccharide Production by Streptococcus mutans and Actinomyces viscosus, J Dent Res60:1601-1610, 1981.
32.
Van Houte, J.: Relationship Between Carbohydrate Intake and Polysaccharide Storing Microorganisms in Dental Plaque, Arch Oral Biol9:91-93, 1964.
33.
Van Houte, J.; De Moor, C.E.; and Jansen, H.M.: Synthesis of lodophilic Polysaccharide by Human Oral Streptococci , Arch Oral Biol15:263-266, 1970.
34.
Van Houte, J.; Winkler, K.C.; and Jansen, H.M.: Iodophilic Polysaccharide Synthesis, Acid Production and Growth in Oral Streptococci , Arch Oral Biol14:45-61, 1969.
35.
Weiss, S.; King, W.J.; Kestenbaum, R.C.; and Donohue, J.J.: Influence of Various Factors on Polysaccharide Synthesis in S. mitis , Ann NYAcad Sci131:839-850, 1965.
36.
Yung, S.G. and Preiss, J.: Biosynthesis of Bacterial Glycogen: Purification and Structural and Immunological Properties of Rhodopseudomonas sphaeroides ADP Glucose Synthetase , J Bacteriol151:742-749, 1982.
