Incisional Biopsy and Seeding in Hamster Cheek Pouch Carcinoma

Abstract

Surgical blades were contaminated by doing incisional biopsies on DMBA-induced cheek pouch carcinomas in hamsters. Contaminated blades were then used to: (1) make cytologic smears; (2) make incisions in the mucosal surface of the lower lip; (3) make incisions in the ventral surface of the tongue; (4) make incisions in the untreated left cheek pouch; and (5) make incisions in the pre-shaved back. One group of animals was killed three hr post-surgery. The second group was killed seven wk post-surgery. Tissues were excised and processed for microscopic study. Malignant cells were found in many of the incision sites of animals from the first group. In the animals of the second group, several tumors growing from the transplanted tumor cells were observed in the incision sites of the lower lips and the tongue. None was observed in the incision sites of the left cheek pouch or the back. Cervical lymph node metastases were found in approximately 50% of the animals of the second group.

Get full access to this article

View all access options for this article.

References

"Cancer Facts and Figures." Washington, DC: American Cancer Society, 1983.

Craig, G.T. : Metastasis from DMBA-Induced Carcinomas in Hamster Cheek Pouch. In: Metastasis Clinical and Experimental Aspects, Developments in Oncology, Vol. 4, K. Hillman , P. Hilgard , and S. Eccles , Eds., The Hague: Martinus Nijhoff, 1980 , pp. 50-54.

Eisenberg, E. : Neoplasia following Cessation of (DMBA) Application to Hamster Buccal Pouch , J Dent Res 56:1430, 1977.

Eveson, J. and MacDonald, D. : Hamster Tongue Carcinogenesis. I: Characteristics of the Experimental Model, J Oral Pathol 10:322-331, 1981.

Freedman, A. and Shklar, G. : Alcohol and Hamster Buccal Pouch Carcinogenesis, Oral Surg 46:794-805, 1978.

Knox, C.L. : Trauma and Tumors, Arch Pathol 7:274-309, 1929.

Levij, I. ; Polliack, A. ; and Thorgeirsson, T. : Correlation of Cytologic Smear and Histologic Findings During -9,10-Dimethyl-1,2-Benzanthracene Induced Carcinogenesis in the Hamster Cheek Pouch, Arch Oral Biol 12:859-863, 1967.

MacDonald, G.D. : Comparison of Epithelial Dysplasia in Hamster Cheek Pouch Carcinogenesis and Human Oral Mucosa, J Oral Pathol 10:185-191, 1981.

Morris, A. : Factors Influencing Experimental Carcinogenesis in the Hamster Cheek Pouch , J Dent Res 40:3-15, 1961.

10.

Renstrup, G. ; Smulow, J. ; and Glickman, I. : Effect of Chronic Methanical Irritation on Chemically Induced Carcinogenesis in the Hamster Cheek Pouch, JADA 64:770-777, 1962.

11.

Rosai, J. and Ackerman, L. : The Pathology of Tumors, Part IV: Behavior and Therapy of Tumors. American Cancer Society: Professional Education Publication, 1979, pp. 45-55.

12.

Rwomushana, J. ; Polliack, A. ; and Levij, I. : Cervical Lymph Node Metastasis of Hamster Cheek Pouch Carcinoma Induced with DMBA, J Dent Res 49:184, 1970.

13.

Salley, J.J. : Experimental Carcinogenesis in the Cheek Pouch of Syrian Hamster, J Dent Res 33:253-262, 1954.

14.

Santis, H. ; Shklar, G. ; and Chauncey, H.H. : Histochemistry of Experimental Induced Leukoplakia and Carcinoma of the Hamster Buccal Pouch, Oral Surg 17:207-218, 1964.

15.

Shklar, G. : Cortisone and Hamster Buccal Pouch Carcinogenesis, Cancer Res 26:2461-2463, 1966.

16.

Shklar, G. : The Effect of Manipulation and Incision on Experimental Carcinoma of Hamster Buccal Pouch, Cancer Res 28: 2180-2181, 1968 .

17.

Shklar, G. : Experimental Oral Pathology in the Syrian Hamster, Progr Exp Tumor Res 16:518-538, 1972.

18.

Stuteville, 0. : Personal communication with N.K. Wood , 1976. Tyzzer, E.E. : Factors in the Production and Growth of Tumor Metastases, J Med Res 28:309-333, 1913.

19.

Warpeha, L. : Personal communication with N.K. Wood , 1983. Wood, C.F. : The Experimental Pathology of Cancer, JAMA 84: 4-8, 1925.