.4 periodontal organ culture system capable of receiving orthodontic type forces was developed. Histalogical, radioautographical, collagen, and prostaglandin synthetic data demonstrated the vitality of the organ over a 24-hour period of culture. Significant increases in the proportion of type III collagen synthesized during periods of active stress were found, but no alterations in relative levels of prostaglandins synthesized during periods of force application were discernible.
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References
1.
Reitan, K.: Biomechanical Principles and Reactions. In: Current Orthodontic Concepts and Techniques, Graber, T.M. and Swain, B.F., Eds., Philadelphia: W.B. Saunders Co., 1975, pp. 111-229.
2.
Sandstedt, C. : Einige Beitrage zur Theorie der Zahnregulierung . Nord Tandl Tidsskr4, 1904. As cited by A.M. Schwartz (1932): Tissue Changes Incidental to Orthodontic Tooth Movement, Int J Orthod18:331-352.
3.
Sandstedt, C. : Einige Beitrage zur Theorie der Zahnregulierung . Nord Tandl Tidsskr1, 1905a. As cited by A.M. Schwartz (1932): Tissue Changes Incidental to Orthodontic Tooth Movement, Int J Orthod18:331-352.
4.
Sandstedt, C. : Einige Beitrage zur Theorie der Zahnregulierung . Nord Tandl Tidsskr2, 1905b. As cited by A.M. Schwartz (1932): Tissue Changes Incidental to Orthodontic Tooth Movement, Int J Orthod18:331-352.
Zengo, A.N.; Pawluk, R.J.; and Bassett, C.A.L.: Stress-induced Bioelectric Potentials in the Dentoalveolar Complex , Am J Orthod64 :17-27, 1973.
7.
Zengo, A.N.; Bassett, C.A.L.; Pawluk, R.J.; and Prountzos, G.: In vivo Bioelectric Potentials in the Dentoalveolar Complex, Am J Orthod66 :130-139, 1974.
8.
Davidovitch, Z. and Shanfeld, J.L.: Cyclic AMP Levels in Alveolar Bone of Orthodontically Treated Cats, Arch Oral Biol20:567-574, 1975.
9.
Yamasaki, K. ; Miura, F.; and Suda, T.: Prostaglandin as a Mediator of Bone Resorption Induced by Experimental Tooth Movement in Rats, J Dent Res59:1635-1642, 1980 .
10.
Bornstein, P. and Traub, W.: The Chemistry and Biology of Collagen . In: The Proteins, Neurath, H. and Hill, R., Eds., 3rd ed., Vol. 4, New York: Plenum Press, 1979, pp. 412-632.
11.
Tashjian, A.M. , Jr.; Voelkel, E.F.; Levine , L.; and Goldhaber, P.: Evidence that the Bone Resorption Stimulating Factor Produced by Mouse Fibrosarcoma Cells is Prostaglandin E2. A New Model for the Hypercalcaemia of Cancer, J Exp Med136:1329-1343, 1972 .
12.
Goodson, J.M. ; Dewhirst, F.E.; and Brunetti , A.: Prostaglandin E2 Levels and Human Periodontal Disease, Prostaglandins6:81-85, 1974.
13.
Goldhaber, P.; Rabadjija, L.; Beyer , W.R.; and Kornhauser , A.: Bone Resorption in Tissue Culture and Its Relevance to Periodontal Disease, JADA87:1027-1033, 1974.
14.
Raisz, L.G. and Koolemans-Beynen , A.R.: Inhibition of Bone Collagen Synthesis by Prostaglandin E2 in Organ Culture, Prostaglandins8:377-385, 1974.
15.
Harell, H.; Dekel, S.; and Binderman, I.: Biochemical Effect of Mechanical Stress on Cultured Bone Cells, Calcif Tissue Res22:202-207, 1977.
16.
Binderman, I. and Cox, J.C.: Effect of Mechanical Stress on Cultured Periosteum Cells: Stimulation of DNA Synthesis, J Dent Res56:B73, 1977.
17.
Somjen, D.; Bindermann, I.; Berger, E.; and Harell, A.: Bone Remodelling Induced by Physical Stress is Prosta
Yen, E.H.K. and Melcher, A.H.: A Continuous Flow Culture System for Organ Culture of Large Explants of Adult Tissue. Effect of Oxygen Tension on Mouse Molar Periodontium, In Vitro14:811-818, 1978.
20.
Laemmli, U.K. : Cleavage of Structural Proteins During the Assembly of the Head of the Bacteriophage, Nature227 :680-685,1970.
21.
Sykes, B.; Puddle, B.; Francis, M.; and Smith, R.: The Estimation of Two Collagens from Human Dermis by Interrupted Gel Electrophoresis , Biochem Biophys Res Commun72:1472-1480, 1976.
22.
Bonner, W.M. and Laskey, R.A.: A Film Detection Method for Tritium Labelled Proteins and Nucleic Acids in Polyacrylamide Gels, Eur J Biochem46:83-88, 1974.
23.
Melcher, A.H. ; Turnbull, R.S.; and Hiddleston , W.J.: In vitro Culture of Periodontium of Young Adult Mouse, Arch Oral Biol18:933-939, 1973.
24.
Turnbull, R.S. and Melcher, A.H.: A Method for Maintaining Large Explants of Mature Mixed Tissue in Organ Culture: Molar Tooth and Supporting Structures from the Mouse, In Vitro10:6-11, 1974.
25.
Melcher, A.H. and Turnbull, R.S.: Organ Culture in Studies on the Periodontium . In: Organ Culture in Biomedical Research , Balls, M. and Monnickendam, M.A., Eds., Cambridge, U.K .: Cambridge University Press, 1976, pp. 149-163.
26.
Sodek, J.: A New Approach to Assessing Collagen Turnover by Using a Microassay. A Highly Efficient and Rapid Turnover of Collagen in Rat Periodontal Tissues , Biochem J160: 243-246, 1976.
27.
Trowell, O.A. : The Culture of Mature Organs in a Synthetic Medium, Exp Cell Res16:118-147, 1959.
28.
Macdougall, J.D.B. and Coupland , R.E.: Organ Culture Under Hyperbaric Oxygen, Exp Cell Res45:385-398, 1967.
29.
Chung, E. and Miller, E.J.: Collagen Polymorphism: Characterization of Molecules with the Chain Composition (α1 (III))3 in Human Tissues, Science183:1200-1201, 1974.
30.
Gay, S. ; Viljanto, J.; Raekallio , J.; and Penttinen, R.: Collagen Types in Early Phases of Wound Healing in Children, Acta Chir Scand144:205-211, 1978.
31.
Eyre, D.R. and Muir, H.: Type III Collagen: A Major Constituent of Rheumatoid and Normal Human Synovial Membrane, Conn Tissue Res4:11-16, 1975.
32.
Weiss, J.B. ; Shuttleworth, C.A.; Brown , R.; Se-Dowfia, K.; Baildam, A.; and Hunter, J.A.: Occurrence of Type III Collagen in Inflamed Synovial Membranes: A Comparison Between Non-rheumatoid, Rheumatoid and Normal Synovial Collagens, Biochem Biophys Res Commun65:907-912,1975.
33.
Butler, W.T. ; Birkedal-Hansen , H.; Beegle, W.F.; Taylor, R.E.; and Chung, E.: Proteins of the Periodontium : Identification of Collagens with the [α1 (I)] 2, α2 and [α1 (III)] 3 Structures in Bovine Periodontal Ligament, J Biol Chem250:8907-8912,1975.
34.
Raisz, L.G. ; Vanderhoek, J.Y.; Simmons , H.A.; Kream, B.E.; and Nicolaou , K.C.: Prostaglandin Synthesis by Foetal Rat Bone in vitro: Evidence for a Role of Prostacyclin , Prostaglandins17:905-914, 1979.
35.
Sandberg, A.L. ; Raisz, L.G.; Goodson , J.M.; Simmons, H.A.; and Mergenhagen , S.E.: Initiation of Bone Resorption by the Classical and Alternative C Pathways and Its Mediation by Prostaglandins, J Immunol119:1378-1381, 1977.
36.
Dowsett, M. ; Eastman, A.R.; Easty , D.M.; Easty, G.C.; Powles, T.J.; and Neville, A.M.: Prostaglandin Mediation of Collagenase-induced Bone Resorption, Nature263:72-74, 1976.
37.
Tashjian, A.H. ; Ivey, J.L.; Delclos , B.; and Levine, L.: Stimulation of Prostaglandin Production in Bone by Phorbol Diesters and Melittin, Prostaglandins16:221-232, 1978.
38.
Heersche, J.N.M. and Jez, D.H.: The Effect of Imizadole-Analogues on Bone Resorption in vitro: A Suggested Role for Thromboxane A2, Prostaglandins21:401-411, 1981.
