Morphogenesis (germ-tube formation) in Candida albicans was induced gratuitously by N-acetylhexosamine derivatives (N-acetylglucosamine covalently linked to agarose, N-acetylmannosamine, hyaluronic acid, colloidal chitin, and mucin). These compounds were not taken up by the yeast cells and did not support growth. 2-Deoxyglucose was a potent inhibitor of germ-tube formation (50 μM), but did not affect the yeast growth yield at a concentration of 2.5 mM. 2-Deoxyglucose covalently linked to agarose did not affect germ-tube formation, and the inhibition by free 2-deoxyglucose was overcome by the addition of glucose to the germ-tube-forming cells. Glucose competitively inhibited 2-deoxyglucose uptake (Ki = 0.14 mM), and these data indicate that 2-deoxyglucose acted intracellularly.
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