A technique for collecting various levels of plaque from incipient occlusal fissure caries was used to evaluate the antibacterial properties of a solution containing I2-KI, CuSO4, and ethanol. In comparison with control fissures (n = 6), the treated fissures (n = 9) showed a 98% difference in recoverable bacteria, with the killing of S. mutans, Actinomyces, and lactobacilli appearing to reflect the level of agent penetration.
Get full access to this article
View all access options for this article.
References
1.
Buonocore, M.G. : Adhesives for Pit and Fissure Caries Control , Dent Clin N Amer16:693-708, 1972.
2.
Ripa, L.W.: Studies in Pits and Fissures. In: The Use of Adhesives in Dentistry , Buonocore, M.G., Ed., Springfield, IL: Charles C. Thomas , 1975, pp. 120-153.
3.
Ripa, L.W.: Occlusal Sealants: Rationale and Review of Clinical Trials, Int Dent J30:127-139, 1980.
4.
Jordan, H.V. and De Paola, P.F.: Effects of Topically Applied 3% Vancomycin Gel on Streptococcus mutans on Different Tooth Surfaces, J Dent Res53:115-120, 1974.
5.
Jordan, H.V. and De Paola, P.F.: Effect of Prolonged Topical Application of Vancomycin on Human Oral Streptococcus mutans Populations, Arch Oral Biol22:193-199, 1977.
6.
Loesche, W.J. ; Hockett, R.N.; and Syed, S.A.: Reduction in Proportions of Dental Plaque Streptococci Following a 5 Day Topical Kanamycin Treatment, J Periodont Res12: 1-10, 1977.
7.
Loesche, W.J. ; Bradbury, D.R.; and Woolfolk , M.P.: Reduction of Dental Decay in Rampant Caries Individuals Following Short-Term Kanamycin Treatment , J Dent Res56:254-265,1977.
8.
Loesche, W.J. ; Syed, S.A.; Murray , R.J.; and Mell-Berg , J.R.: Effect of Topical Acidulated Phosphate Fluoride on Percentage of Streptococcus mutans and Streptococcus sanguis in Plaque II. Pooled Occlusal and Pooled Approximal Samples, Caries Res9:139-155, 1975.
9.
Caufield, P.W. and Gibbons, R.J.: Suppression of Streptococcus mutans in the Mouths of Humans by a Dental Prophylaxis and Topically-applied Iodine , J Dent Res58 :1317-1326, 1979 .
10.
Caufield, P.W. : Combined Effect of Iodine and Sodium Fluoride on Dental Caries in Rats and on the Viability of Streptococcus mutans in vitro , Caries Res15:484-491, 1981.
11.
Caufield, P.W. ; Navia, J.M.; Rogers , A.M.; and Al-Varez , C.: Effect of Topically-applied Solutions of Iodine, Sodium Fluoride or Chlorhexidine on Oral Bacteria and Dental Caries in Rats, J Dent Res60:927-932, 1981.
12.
Meiers, J.C. ; Wirthlin, M.R.; and Shklair, I.L.: A Microbiological Analysis of Human Early Carious and Non-carious Fissures, J Dent Res61:460-464, 1982.
13.
Radike, A.W.: Criteria for Diagnosis of Dental Caries, Proceedings of the Conference on the Clinical Testing of Cariostatic Agents , Chicago: American Dental Association , 1972.
14.
Rogosa, M.; Mitchell, J.A.; and Wiseman, R.F.: A Selective Medium for the Isolation and Enumeration of Oral and Fecal Lactobacilli , J Bacteriol62:132-133, 1951.
15.
Jordan, H.V. ; Krause, B.; and Moller, A.: A Method for Sampling Human Dental Plaque for Certain "Caries-inducing" Streptococci, Arch Oral Biol13:919-927, 1968.
16.
Gold, O.G.; Jordan, H.V.; and Van Houte, J.: A Selective Medium for Streptococcus mutans, Arch Oral Biol18: 1357-1364, 1973.
17.
Zylber, L.J. and Jordan, H.V.: Development of a Selective Medium for Detection and Enumeration of Actinomyces viscosus and Actinomyces naeslundii in Dental Plaque, J Clin Microbiol15:253-259, 1982.
18.
Caufield, P.W. and Wannemuehler , Y.M. : In vitro Susceptibility of Streptococcus mutans 6715 to Iodine and Sodium Fluoride, Singly and in Combination, at Various pH Values, Antimicrob Agents Chemother22:115-119, 1982.
19.
Hanke, M.T. : Studies on the Local Factors in Dental Caries. I. Destruction of Plaques and Retardation of Bacterial Growth in the Oral Cavity, JADA27:1379-1393, 1940 .
20.
Forbes, J.C. and Smith, J.D.: Studies on the Effect of Metallic Salts on Acid Production in Saliva. I., J Dent Res31:129-131, 1952.
21.
Oppermann, R.V. : The Effect of Organic and Inorganic Ions on the Acidogenicity of Dental Plaque. In: Tooth Surface Interactions and Preventive Dentistry, G. Rolla, T. Sonju, and G. Embery , Eds., London, England: Information Retrieval, Limited, 1981, pp. 3-16.
22.
Loë, H. ; Karring, T.; and Theilade, E.: An in vivo Method for the Study of the Microbiology of Occlusal Fissures, Caries Res7:120-129, 1973.
23.
Theilade, J. ; Fejerskov, 0. ; and Horsted, M.: A Transmission Electron Microscope Study of Seven-day-old Bacterial Plaque in Human Tooth Fissures, Arch Oral Biol21 :587-598, 1976.
24.
Theilade, E. ; Fejerskov, 0.; Karring, T.; and Theilade, J.: A Microbiological Study of Old Plaque in Occlusal Fissures Implanted in Human Teeth, Caries Res12:313-319, 1978.
25.
Thott, E.K. ; Folke, L.E.A.; and Sveen, O.B.: A Microbiological Study of Human Fissure Plaque, Scand J Dent Res82:428-436, 1974.
26.
Edwardsson, S.: Bacteriological Studies on Deep Areas of Carious Dentine, Odontol Revy25:1-143, 1974.
27.
Loesche, W.J. and Syed, S.A.: The Predominant Cultivable Flora of Carious Plaque and Carious Dentine, Caries Res7: 201-216, 1973.
