Studies on Chondrocytes from Mandibular Condylar Cartilage,Nasal Septal Cartilage,and Spheno-occipital Synchondrosis in Culture. I. Morphology,Growth,Glycosaminoglycan Synthesis,and Responsiveness to Bovine Parathyroid Hormone (1-34)
Restricted accessResearch articleFirst published online January, 1984
Studies on Chondrocytes from Mandibular Condylar Cartilage,Nasal Septal Cartilage,and Spheno-occipital Synchondrosis in Culture. I. Morphology,Growth,Glycosaminoglycan Synthesis,and Responsiveness to Bovine Parathyroid Hormone (1-34)
Methods for isolating chondrocytes from the craniofacial complex and culturing them in vitro were established. Chondrocytes which were isolated by collagenase digestion from mandibular condylar cartilage, nasal septal cartilage, and spheno-occipital synchondrosis grew well in vitro. All three types of chondrocytes actively synthesized glycosaminoglycans, a differentiated phenotype of chondrocytes, and responded well to parathyroid hormone. However, some different characteristics were noted among the three types of chondrocytes in culture.
Get full access to this article
View all access options for this article.
References
1.
Baume, L.J.: Principles of Cephalofacial Development Revealed by Experimental Biology , Am J Orthod47:881-901, 1961.
2.
Durkin, J.F. ; Heeley, J.D.; and Irving, J.T.: The Cartilage of the Mandibular Condyle , Oral Sci Rev2:29-99, 1973.
3.
Koski, K.: Cartilage in the Face. In: Morphogenesis and Malformation of Face and Brain, Bergsma, D., Ed., New York, NY: Alan R. Liss, Inc., 1975, pp. 231-254.
4.
Sarnat, B.G. : Growth of Bones as Revealed by Implant Markers in Animals, Am J Phys Anthropol29:255-286, 1968.
Rönning, 0.: Observations on the Intracerebral Transplantation of the Mandibular Condyle , Acta Odontol Scand24 :443-457, 1966.
7.
Okada, M.: An Experimental Study on the Rat Mandibular Condyle by Means of Subcutaneous Transplantation, J Osaka Univ Dent Soc22:21-45, 1977.
8.
Meikle, M.C. : In vivo Transplantation of the Mandibular Joint of the Rat: An Autoradiographic Investigation into Cellular Changes at the Condyle, Arch Oral Biol18:1011-1020, 1973 .
9.
Kvinnsland, S.: Growth in Height of Autotransplanted Nasal Septum in the Rat, Acta Odontol Scand31:317-322, 1973.
10.
Kvinnsland, S. and Kvinnsland , S.: Transplantation Studies of Nasal Septal Cartilage in Rats, Craniofacial Growth Series, No. 6, Ann Arbor: The University of Michigan, 1976, pp. 239-249.
11.
Shimomura, Y.; Yoneda, T.; and Suzuki, F.: Osteogenesis by Chondrocytes from Growth Cartilage of Rat Rib, Calcif Tissue Res19:179-187, 1975.
12.
Suzuki, F.; Yoneda, T.; and Shimomura, Y.: Calcitonin and Parathyroid-hormone Stimulation of Acid Mucopolysaccharide Synthesis in Cultured Chondrocytes Isolated from Growth Cartilage, FEBS Lett70:155-158, 1976.
13.
Suzuki, F.; Takase, T. ; Takigawa, M.; Uchida, A.; and Shimomura, Y.: Simulation of the Initial Stage of Endochondral Ossification: In vitro Sequential Culture of Growth Cartilage Cells and Bone Marrow Cells, Proc Natl Acad Sci USA78:2368-2372, 1981 .
14.
Takigawa, M. ; Watanabe, R.; Ishida , H.; Asada, A.; and Suzuki, F.: Induction by Parathyroid Hormone of Ornithine Decarboxylase in Rabbit Costal Chondrocytes in Culture, J Biochem85:311-314, 1979.
15.
Takigawa, M. ; Ishida, H.; Takano , T.; and Suzuki, F.: Polyamine and Differentiation: Induction of Ornithine Decarboxylase by Parathyroid Hormone is a Good Marker of Differentiated Chondrocytes, Proc Natl Acad Sci USA77 :1481-1485, 1980.
16.
Takigawa, M. ; Takano, T.; and Suzuki, F.: Effects of Parathyroid Hormone and Cyclic AMP Analogues on the Activity of Ornithine Decarboxylase and Expression of the Differentiated Phenotype of Chondrocytes in Culture , J Cell Physiol106:259-268, 1981.
17.
Takigawa, M. ; Takano, T.; and Suzuki, F.: Restoration by Cyclic AMP of the Differentiated Phenotype of Chondrocytes from De-differentiated Cells Pretreated with Retinoids , Mol Cell Biochem42:145-153, 1982.
18.
Kato, Y.; Nasu, N.; Takase, T.; Daikuhara, Y.; and Suzuki, F.: A Serum-free Medium Supplemented with Multiplication-stimulating Activity (MSA) Supports Both Proliferation and Differentiation of Chondrocytes in Primary Culture, Exp Cell Res125:167-174, 1980.
19.
Lowry, O.H. ; Rosebrough, N.J.; Farr , A.L.; and Ran-Dall , R.J.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem193:265-275, 1951.
20.
Uchida, A.; Watanabe, R.; Takase, T.; Kobayashi, S.; Shimomura, Y.; and Suzuki, F.: In vitro Culture of Chondrocytes Isolated from Growth and Resting Cartilage, III, Kotsu Taishya (Bone Metab)11:261-268; 1978.
21.
Corvol, M.T. ; Dumontier, M.F.; Rappaport , R.; Guyda, H.; and Posner, B.I.: The Effect of a Slightly Acidic Somatomedin Peptide (ILAs) on the Sulfation of Proteoglycans from Articular and Growth Plate Chondrocytes in Culture, Acta Endocrinol89:263-275, 1978.
22.
Sicher, H.: Growth of the Mandible, Am J Orthod33:30-35, 1947.
23.
Moss, M.L.: Functional Analysis of Human Mandibular Growth, J Prosthet Dent10:1149-1159, 1960.
