Gramicidin, an ionophoric antibiotic which enhances proton permeability of cell membranes, was found to increase the acid sensitivity of glycolysis by intact cells of Streptococcus mutans GS-5, to inhibit uptake of 2-deoxyglucose at pH values of 5.5 or less, and to cause a decrease in the intracellular levels of early intermediates of glycolysis. The inhibitory effects of the antibiotic on glycolysis at low pH values were related to inhibition of sugar uptake via the phosphotransferase system.
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