The attachment of Actinomyces naeslundii ATCC 12104 to human buccal epithelial cells pre-treated with neuraminidase (sialidase) was evaluated. Both commercial clostridial neuraminidase and neuraminidase preparations from the test strain of A. naeslundii enhanced attachment. The results suggest that the A. naeslundii β-galactoside-seeking ligand involved in hemagglutination and interbacterial coaggregation also mediates one type of binding to human buccal epithelial cells.
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References
1.
Socransky, S.S. ; Hubersak, C.; and Propas, D.: Induction of Periodontal Destruction in Gnotobiotic Rats by Human Oral Strain of Actinomyces naeslundii, Arch Oral Biol15 :993-995, 1970.
2.
Jordan, H.V. ; Keyes, P.H.; and Bellack, S.: Periodontal Lesions in Hamsters and Gnotobiotic Rats Infected with Actinomyces of Human Origin, J Periodont Res7 :21-28, 1972.
3.
Ellen, R.P.: Establishment and Distribution of Actinomyces viscosus and Actinomyces naeslundii in the Human Oral Cavity, Infect Immun14:1119-1124, 1976.
4.
Ellen, R.P.; Segal, D.N.; and Grove, D.A.: Relative Proportions of Actinomyces viscosus and Actinomyces naeslundii in Dental Plaques Collected from Single Sites, J Dent Res57:550, 1978.
5.
Costello, A.H. ; Cisar, J.O.; Kolenbrander , P.E.; and Gabriel, 0.: Neuraminidase-Dependent Hemagglutination of Human Erythrocytes by Human Strains of Actinomyces viscosus and Actinomyces naeslundii, Infect Immun26 :563-572, 1979.
6.
Ellen, R.P.; Fillery, E.D.; Chan, K.H.; and Grove, D.A.: Sialidase-Enhanced Lectin-Like Mechanism for Actinomyces viscosus and Actinomyces naeslundii Hemagglutination, Infect Immun27:335-343, 1980.
7.
Ellen, R.P.; Walker, D.L.; and Chan, K.H.: Association of Long Surface Appendages with Adherence-Related Functions of the Gram-Positive Species Actinomyces naeslundii, J Bacteriol134:1171-1175, 1978.
8.
Saunders, J.M. and Miller, C.H.: Attachment of Actinomyces naeslundii to Human Buccal Epithelial Cells, Infect Immun29:981-989, 1980.
9.
Miller, C.H. ; Palenik, C.J.; and Stamper, K.E.: Factors Affecting the Aggregation of Actinomyces naeslundii During Growth and in Washed Cell Suspensions, Infect Immun21: 1003-1009, 1978.
10.
Warren, L.: The Thiobarbituric Acid Assay of Sialic Acids, J Biol Chem234:1971-1975, 1956.
11.
Cassidy, J.T. ; Jourdian, G.W.; and Roseman, S.: Sialidase from Clostridium perfringens. In: Methods in Enzymology, Vol. 8 , Neufeld, E. and Ginsburg, V., Eds. New York, NY: Academic Press, 1966, pp. 680-685.
12.
Cisar, J.O. ; Kolenbrander, P.E.; and McIntire , F.C.: Specificity of Coaggregation Reactions Between Human Oral Streptococci and Strains of Actinomyces viscosus or Actinomyces naeslundii, Infect Immun24:742-752, 1979.
13.
Kolenbrander, P.E. and Williams, B.L.: Lactose-Reversible Coaggregation between Oral Actinomycetes and Streptococcus sanguis, Infect Immun33:95-102, 1981.
14.
Yamazaki, Y. ; Ebisu, S.; and Okada, H.: Eikenella corrodens Adherence to Human Buccal Epithelial Cells, Infect Immun31:21-27, 1981.
15.
Gibbons, R.J. and Qureshi, J.V.: Inhibition of Adsorption of Streptococcus mutans Strains to Saliva-Treated Hydroxyapatite by Galactose and Certain Amines , Infect Immun26: 1214-1217, 1979.
