An acetate buffer system, supersaturated with fluorapatite but not with hydroxyapatite, produced lesions in abraded bovine enamel which resembled human enamel caries lesions, both morphologically and in their F distribution. A gelatin-lactate system gave similar results when unpurified gelatin was used. Systems containing deionized gelatin, diphosphonate, or Ca and phosphate but no F, failed to produce caries-like lesions.
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References
1.
Applebaum, E. : The Radiopaque Surface Layer of Enamel and Caries, J Dent Res19:41-46, 1940.
2.
Darling, A.I. : Studies of the Early Lesion of Enamel Caries with Transmitted Light, Polarised Light and Radiography. Part II, Br Dent J101:329-341, 1956.
3.
Guzman, C.; Brudevold, F.; and Mermagen, H.: A Soft Roentgen-Ray Study of Early Carious Lesions, JADA55:509-515,1957.
4.
Bergman, G. and Lind, P.O.: A Quantitative Microradiographic Study of Incipient Enamel Caries , J Dent Res45:1477-1484, 1966 .
5.
Frank, R.M.; Capitant, M.; and Goni, J.: Electron Probe Studies of Human Enamel, J Dent Res45:672-682, 1966.
6.
Silverstone, L.M.: Structure of Carious Enamel, Including the Early Lesion. In: Oral Sciences Review, No. 2, Melcher, A.H. and Zarb, G.A., Eds., Copenhagen:Munksgaard, 1973, pp. 100-160.
7.
Hallsworth, A.S.; Weatherell, J.A.; and Robinson , C.: Fluoride Uptake and Distribution in Human Enamel during Carious Attack, Caries Res9:294. 1975.
8.
Hallsworth, A.S.; Weatherell, J.A.; and Deutsch, D.: Determination of Subnanogram Amounts of Fluoride with the Fluoride Electrode, Anal Chem48:1660-1664, 1976.
9.
Tohda, H.: Forming Mechanism of Subsurface Carious Lesions in Enamel, Shikwa Gakuho81:421-444, 1981.
10.
Silverstone, L.M. and Featherstone , M.J.: Crystal Diameters in the Four Histological Zones of Early Caries, IADR Progr & Abst61 :No. 279, 1982.
11.
Aoba, T.; Moriwaki, Y.; Doi, Y.; Okazaki, M. ; Takahashi, J.; and Yagi, T.: The Intact Surface Layer in Natural Enamel Caries and Acid-Dissolved Hydroxyapatite Pellets. An X-ray Diffraction Study, J Oral Pathol10:32-39, 1981.
12.
Pigman, W. and Sognnaes, R.F.: Histologic Studies of Caries-Like Lesions Produced in the Artificial Mouth , Oral Surg8:530-538, 1955.
13.
Coolidge, T.B. ; Besic, F.C.; and Jacobs, M.H.: A Microscopic Comparison of Clinically and Artificially Produced Changes in Enamel, Oral Surg8:1204-1210, 1955.
14.
Sperber, G.H. and Buonocore, M.G.: Effects of Different Acids on Character of Demineralization of Enamel Surfaces, J Dent Res42:707-723, 1963.
Groeneveld, A. and Arends, J.: Influence of pH and Demineralization Time on Mineral Content, Thickness of Surface Layer and Depth of Artificial Caries Lesions, Caries Res9:36-44, 1975.
17.
Glimcher, M.J. ; Friberg, U.A.; and Levine, P.T.: The Identification and Characterisation of a Calcified Layer of Coronal Cementum in Erupted Bovine Teeth. In: Tooth Enamel, Stack, M.V. and Fearnhead, R.W., Eds., Bristol: John Wright & Sons, Ltd., 1965, pp. 70-73.
18.
Larsen, M.J. : Chemically Induced in vitro Lesions in Dental Enamel, Scand J Dent Res82:496-509, 1974.
19.
Silverstone, L.M.: The Primary Translucent Zone of Enamel Caries and of Artificial Caries-Like Lesions, Br Dent J120:461-471, 1966.
20.
Zahradnik, R.T.; Propas, D.; and Moreno, E.C.: Effect of Fluoride Topical Solutions on Enamel Demineralization by Lactate Buffers and Streptococcus mutans in vitro , J Dent Res57:940-946, 1978.
21.
Sundstrom, B.: A Technique of Preparing Thin Ground Sections of Hard Tissues: Tooth and Bone, Acta Odontol Scand24:159-178, 1966.
22.
Chen, P.S.; Toribara, T.Y.; and Warner, H.: Micro-determination of Phosphorus, Anal Chem28:1756-1758, 1956.
23.
Travis, D.F. : The Comparative Ultrastructure and Organization of Five Calcified Tissues . In: Biological Calcification, Schraer, H., Ed., New York: Appleton-Century-Crofts , 1970, pp. 203-311.
24.
Brown, W.A.B. ; Christofferson, P.V.; Massler , M.; and Weiss, M.B.: Postnatal Tooth Development in Cattle, Am J Vet Res21:7-34, 1960.
25.
Koulourides, T.: Effect of 1 ppm Fluoride on the Microradiographic Profile of Experimental Enamel Lesions, IADR Progr & Abst56:No. 269, 1977.
26.
Larsen, M.J. : Demineralization of Human Enamel, Scand J Dent Res82:491-495, 1974.
27.
Kidd, E.A.M. and Joyston-Bechal , S.: Histopathological Appearance of Caries-Like Lesions of Enamel Created Artificially in vitro in Acidified Gels Containing Fluoride, Caries Res14:40-44, 1980.
28.
Von Bartheld, F.: Membrane Phenomena in Carious Dissolution of the Teeth, Arch Oral Biol6:284-303, 1961.
29.
Arends, J. and Schuthof, J.; Microhardness and Lesion Depth Studies of Artificial Caries Lesions: A Comparison of Gelatin and HEC Based Systems, J Biol Buccale8:175-181, 1980.
30.
Okazaki, M.; Aoba, T.; Doi, Y.; Takahashi, J.; and Moriwaki, Y.: Solubility and Crystallinity in Relation to Fluoride Content of Fluoridated Hydroxyapatites, J Dent Res60:845-849, 1981.
31.
Francis, M.D.; Briner, W.W.; and Gray, J.A.: Chemical Agents in the Control of Calcification Processes in Biological Systems . In: Hard Tissue Growth, Repair and Remineralization , Elliott, K. and Fitzsimons, D.W., Eds., Amsterdam: Elsevier , 1973, pp. 57-83.
32.
Francis, M.D. : The Inhibition of Calcium Hydroxyapatite Crystal Growth by Polyphosphonates and Polyphosphates, Calcif Tissue Res3:151-162, 1969.
33.
Francis, M.D. and Briner, W.W.: The Effect of Phosphonates on Dental Enamel in vitro and Calculus Formation in vivo, Calcif Tissue Res11:1-9, 1973.
34.
Muhlemann, H.R. and Aeschbacher , M.: Inhibition of Enamel Remineralization by Diphosphonate, Helv Odont Acta14:30, 1970.
