The effects of parathyroid hormone (PTH), calcitonin (CT), 1,25(OH)2D3, and 24,25(OH) 2D3 on calcium transport through the secretory stage enamel organ were studied on developing rat molars in vitro. 24,25(OH)2D 3 increased 45Ca uptake by the explants. 24,25(OH)2 D3 plus PTH further enhanced 45Ca uptake and resulted in an increase in net calcium uptake by the developing enamel.
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References
1.
Hammett, F.S. : Studies of the Thyroid Apparatus. VII. A Differential Effect of Thyro-parathyroidectomy and Parathyroidectomy on the Incisor Teeth of the Albino Rat, Am J Physiol62:197-201, 1922.
2.
Albright, F. and Strock, M.S.: The Association of Acalcification of Dentine with Hypoparathyroidism in Rats and the Cure of Same with Parathormone, with Some Correlated Observations in Man, J Clin Invest12:974, 1933.
3.
Gavin, J.B.: A Comparison of the Incisor Teeth of Intact, Hypophysectomized and Thyroparathyroidectomized Rats, Am J Anat117:159-170, 1965.
4.
Roberts, W.E. : The Effects of Chronic Administration of Parathyroid Extract on the Degree of Calcification of Rat Incisors and Adjacent Alveolar Bone, Chron Omaha Dent Soc29:213-214, 1966.
5.
Gozariu, L.; Barabas, B.; Cristea, M.; and Abra-Ham, A.: Hormonal Reactivity in Tooth Germs in vitro: Effects of Parathyroid Hormone and Calcitonin, Calcif Tissue Res22(Suppl):422-424, 1977.
6.
Kiguel, E.: Effect of Vitamin D Deficiency on Tooth Development, J Dent Res39:672, 1960.
7.
Kiguel, E.: Histochemical Changes in Developing Teeth of Vitamin-deficient Rats, J Dent Res40:685, 1961.
8.
Ferguson, H.W. and Hartles, R.L.: The Effect of Vitamin D on the Dentine of the Incisor Teeth and on the Alveolar Bone of Young Rats Maintained on Diets Deficient in Calcium or Phosphorus, Arch Oral Biol9:447-460, 1964.
9.
Ferguson, H.W. and Hartles, R.L.: The Effect of Diets Deficient in Calcium or Phosphorus in the Presence and Absence of Supplements on Vitamin D on the Incisor Teeth and Bones of Adult Rats,Arch Oral Biol11:1345-1364, 1966.
10.
Harrand, R.B. ; Green, R.M.; and Hartles, R.L.: A Study in the Rat of the Interaction Between the Effects of Calcium and Phosphorus Content of the Diet at Two Different Levels and the Presence or Absence of Vitamin D, Arch Oral Biol20:55-60, 1966.
11.
Hartles, R.L. and Shapiro, I.M.: Changes in the Rat Incisor Teeth in Response to Dietary Deficiencies of Calcium or Phosphorus in the Presence or Absence of Added Vitamin D, Arch Oral Biol11:21-29, 1966.
12.
Kiguel, E.: Alkaline-phosphatase Activity in Developing Rat Molars of Vitamin D-deficient Rats. I. High Calcium-Phosphorus Ratio Diet, J Dent Res43 :71-77, 1964.
13.
Hirsch, P.F. ; Voelkel, E.F.; and Munson, P.L.: Thyrocalcitonin: Hypocalcemic Hypophosphatemic Principle of the Thyroid Gland, Science146:412-413, 1964.
14.
Hicks, E.P. ; Cooper, C.W.; and Waddell, W.J.: Whole-body Autoradiography of the Distribution of 125I-thyrocalcitonin in Mice, J Dent Res50:1307-1313, 1971.
15.
Mallek, H.M. ; Nakamato, T.; Nuchtern, E.; and Miller, S.A.: The Effect of Calcitonin in vitro on Tooth Germs in Protein-energy Malnourished Rats, J Dent Res58: 1921-1926, 1979.
16.
Kline, L.W. : The Effect of Calcitonin on Calcium Uptake in Mouse Molars in vitro, Experimentia37:480-481, 1981.
17.
Bawden, J.W. and Wennberg, A.: In vitro Study of Cellular Influence on 45Ca Uptake in Developing Rat Enamel, J Dent Res56:313-319, 1977.
18.
Bawden, J.W. ; Merritt, D.H.; and Deaton, T.G.: In vitro Study of 45Ca and 32P Uptake in Developing Rat Molar Enamel Using Quantitative Methods, Arch Oral Biol26:477-482, 1981.
19.
Takano, Y. and Crenshaw, M.A.: The Penetration of Intravascularly Perfused Lanthanum into the Ameloblastic Layer of Developing Rat Molar Teeth, Arch Oral Biol25: 505-511, 1980.
20.
Takano, Y. and Crenshaw, M.A.: Ca-ATPase in the Rat Molar Enamel Organ, IADR Progr & Abst60:No. 635, 1981.
21.
Morgan, J.F. ; Morton, H.J.; and Raymon, C.P.: Nutrition of Animal Cells in Tissue Culture. I. Initial Studies on Svnthetic Medium, Proc Soc Exp Biol Med73:1-8, 1950.
22.
Crowell, J.A. , Jr.; Cooper, C.W.; Toverud, S.U.; and Boass, A.: Influence of Vitamin D on Parathyroid Hormone-induced Adenosine 3',5'-monophosphate Production by Bone Cells Isolated from Rat Calvariae , Endocrinology109 :1715-1722,1981.
23.
Boass, A.; Ramp, W.K.; and Toverud, S.U.: Hypocalcemic, Hypophosphatemic Rickets in Rat Pups Suckling Vitamin D-deprived Mothers, Endocrinology109:505-512, 1981.
24.
Kim, Y.S. ; Stumpf, W.E.; Clark, S.A.; Sar, M.; and Deluca, H.F.: Target Cells for 1,25-dihydroxyvitamin D3 in Developing Rat Incisor Teeth, J Dent Res62:58-59, 1983.
25.
Takano, Y.; Crenshaw, M.A.; and Reith, E.J.: Correlation of 45Ca Incorporation with Maturation Ameloblast Morphology in the Rat Incisor, Calcif Tissue Int34:211-213, 1982.
26.
Vicars, T.M. ; Stanfield, C.N.; Crenshaw, M.A.; and Bawden, J.W.: The Effects of ATP Depletion and Ionophore A12387 on Calcium Transport in the Secretory Rat Enamel Organ, Arch Oral Biol, In press.
27.
CRENSHAW, M.A.: Unpublished data.
28.
Crenshaw, M.A. and Takano, Y.: Mechanisms by Which the Enamel Organ Controls Calcium Entry into Developing Enamel, J Dent Res61(Sp. Iss.): 1574-1579, 1982.
29.
Norman, A.W. and Edelstein, S.: Overview of Vitamin D Sessions. In: Current Advances in Skeletogenesis , M. Silberman and H. C. Slavkin, Eds., Amsterdam: Excerpta Medica, 1982, pp. 230-232.
30.
Tanaka, Y.; Deluca, H.F.; Kobayashi, Y.; Taguchi, T.; Ikekawa, N.; and Morisaki, M.: Biological Activity of 24,24-difluoro-25-hydroxyvitamin D3; Effect of Blocking of 24-hydroxylation on the Function of Vitamin D, J Biol Chem254:7163-7167,1979.
31.
Weintroub, S. and Reddi, A.H.: Vitamin D Metabolites and Endochondral Bone Development. In: Current Advances in Skeletogenesis, M. Silberman and H.C. Slavkin , Eds., Amsterdam : Excerpta Medica, 1982, pp. 211-217.
32.
Dziak, R.: Effects of Vitamin D3 Metabolites on Bone Cell Calcium Transport, Calcif Tissue Res26:65-70, 1978.
33.
Eilam, Y.; Szydel, N.; and Harell, A.: Effects of Vitamin D Metabolites on Cellular 45Ca and on Ca Transport in Primary Cultures of Bone Cells, Mol Cell Endocrinol19 :263-273,1980.
34.
Zanetti, M. ; Camerotto, R.; Romeo, D.; and Deber-Nard , D.: Active Extrusion of Ca2+ from Epiphyseal Chondrocytes of Normal and Rachitic Chickens, Biochem J202:303-307, 1982.
